Báo cáo khoa học: Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase a-primase ppt

... Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase a-primase Richard W. P. Smith 1, * and Heinz-Peter Nasheuer 1,2 1 Abteilung Biochemie, Institut fu ¨ r ... polyomavirus JC virus (JCV) can be supported in vitro by DNA polymerase a-primase of either human or murine origin indicating that the mechanism of its...

Ngày tải lên: 17/03/2014, 03:20

... protein–lipid and protein–protein interactions there. Indeed, reconstitution systems using bilayer vesicles yielded higher rates of activity w ith rabbit liver CYP3A6 and CYP2B4 [11,12] and with human ... stoichiometry of 0.9, a nd molar lipid/protein ratio of 1200. The intra- and interday degree of variation in relative P450, reductase, and lipid content did not exceed...

Ngày tải lên: 08/03/2014, 10:20

... activation was measured using a DNA binding assay and a luciferase reporter gene assay. For determina- tion of DNA binding, CRLPs (0.3 lmol triacyl- glycerolÆmL )1 ) were incubated with macrophages ... mL) and incu- bations continued in fetal bovine serum-free culture medium for 24 h in the presence of ApoA-I ⁄ phosphatidylcholine (100 lgÆmL )1 ). At the times indicated in t...

Ngày tải lên: 23/03/2014, 04:20

... role of AP-2a and AP-2e in chondrogenesis. Overview of the differen- tiation stages during chondrogenesis and the involvement of transcription factors (henatoxylin and eosin-stained section of ... for humans and mice. Orthologs of some AP-2s have also been found in frogs and fish, and homologs occur in invertebrates. All AP-2s have a highly conserved basic helix–spa...

Ngày tải lên: 16/02/2014, 09:20

... cathepsins B and D, with the major cleavages generated in vitro occurring mainly within domain III of ETA-A. Cell-free endosomes preloaded in vivo with ETA intraluminally processed and extraluminally ... acidic cathep- sins B and D in increasing the in vitro transfer of the ADP-ribosyl moiety of NAD + to EF-2 by ETA, but not in the release of cytochrome c from ce...

Ngày tải lên: 18/02/2014, 04:20

... expression in Escherichia coli, recombin- ant baculoviruses, recombinant poxviruses, chimeric viruses derived from infectious cDNA clones of DENv, and naked DNA vaccines. In preclinical evaluation using ... DENv infection In the absence of effective antiviral drugs, vaccination offers a good chance for decreasing the incidence of these diseases, live virus vaccines being the...

Ngày tải lên: 19/02/2014, 00:20

... (1994) Binding and modification of proteins by methylglyoxal under physiological conditions. A kinetic and mechanistic study with Nalpha-acetylarginine, Nalpha-acetylcysteine, and Nalpha-acetyllysine, ... Ng R (2001) Suppression of the accumulation of triosephosphates and increased for- mation of methylglyoxal in human red blood cells dur- ing hyperglycaemia by thiamine...

Ngày tải lên: 19/02/2014, 02:20

... adaptors, and cloned into the correspond- ing sites of pEFneo, using the procedure described [20]. Direct DNA sequencing of the construct obtained, per- formed by the dideoxynucleotide chain-termination ... obtained by Bio- Gel P-4 purification of the included volume of the column in (A), large O-glycans (j), obtained as the included volume of the column in (C), and sma...

Ngày tải lên: 19/02/2014, 12:20

... lotting. G-Actin/F-actin in vitro assay Determination o f the amount of filamentous (F-actin) content compared with free globular actin (G-actin) content was performed using the F-actin/G-actin in ... fixed, permeabilized and stained for F-actin with rhodamine-conju- gated phalloidin. Th e effects of toxin B and latrunculin B on the actin filaments was examined by preincubating th...

Ngày tải lên: 19/02/2014, 16:20

... University and distributed by the Linguistic Data Consortium (Grishman, Macleod and Wolf (1993)). Comlex contains detailed syntactic information for about 45,000 English words, including part of ... Conversion of the WordNet KB was also straightforward. WordNet files in Prolog are part of the standard WordNet distribution. Therefore, the bulk of the task involved routin...

Ngày tải lên: 20/02/2014, 18:20