Báo cáo khoa học: Identification of amino acids in antiplasmin involved in its noncovalent ‘lysine-binding-site’-dependent interaction with plasmin pptx

... is involved in the lysine-binding-site-mediated interaction between plasmin and antiplasmin. Molecular modelling of the C-terminal portion of antiplasmin The amino- acid sequence of the C-terminal ... Identification of amino acids in antiplasmin involved in its noncovalent ‘lysine-binding-site’-dependent interaction with plasmin Haiyao Wang, Ann...

Ngày tải lên: 17/03/2014, 03:20

... DNA-binding motif in nucleolin [21– 27]. The RGG domain of FMRP, which is an RNA- binding protein involved in nerve cell differentiation, interacts with the G-quartet forming RNA [22–25]. In addition, ... rate of 2 °CÆmin )1 . Binding reactions were performed in a final volume of 20 lL using 100 fmol of the labeled oligonucleotide and a varying concentration (0– 2.5 lm) of...

Ngày tải lên: 15/02/2014, 01:20

... containing different domains of the protein, to determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. Six GST-fused recombinant proteins containing ... times with NaCl ⁄ P i , and specific binding of the antibody was detected by incubating the sections in a solution of 10 mm diaminobenzidine in 10 mm NaCl ⁄ P i . H. Tohse et al....

Ngày tải lên: 18/02/2014, 17:20

... sugar- binding pocket of At1g21360 very rich in arginines. Fourthly, the residue corresponding to Val209 in human GLTP corresponds to Ser202 in AtGLTP1, a methionine in At1g21360 and a proline in ... sulphu- raria GLTP are also included. Black boxes indicate that identical amino acids are pres- ent in at least four of the sequences, while shaded boxes indicate that amino...

Ngày tải lên: 23/03/2014, 07:20

... binding of the LAO to sialic acid-binding Ig superfamily lectins via its sialylated glycan moiety results in the produc- tion of locally high concentrations of H 2 O 2 in or near the binding interface. ... 5 min. After being run, the gel was stained with a PhastGel protein silver staining kit (GE Healthcare Bio-Science). Analysis of amino- acid sequence Amino- acid sequence...

Ngày tải lên: 30/03/2014, 10:20

... and 25% in pH 7.4 Dulbecco’s NaCl ⁄ P i containing 0.05% gelatin (DPBSG). Bacteria [10 lL containing 10 6 colony-forming units (CFU)] were inoculated into 190 lL reaction wells containing the ... fragment contained a single ORF of 924 or 978 bp with a predicted gene product of 307 or 325 amino acids (Fig. 1). Upstream sequence analysis of lpxX revealed the presence of a gene e...

Ngày tải lên: 18/02/2014, 14:20

... number of genes encoding structural components of the cytoskeleton and proteins involved in contractile functions (including actin, tubulin myosin and profilin) were differentially expressed in C. ... known to involve major adjustments in the expression of ribosomal genes and genes coding for proteins involved in RNA metabolism and protein synthesis. In fact, protein synthe...

Ngày tải lên: 18/02/2014, 16:20

... biosynthesis of triterpene saponins is required, including the enzymes catalyzing the sequence of reactions and the genes encoding these enzymes. The biosynthesis of triterpene saponins involves the initial ... Kirakosyan A, Inoue K, Hiraoka N, Ikeshiro Y, Kushiro T, Shibuya M & Ebizuka Y (2001) Cloning and characterization of a cDNA encod- ing b-amyrin synthase involved in gl...

Ngày tải lên: 19/02/2014, 07:20

... Characterization of the recombinant extracellular domains of human interleukin-20 receptors and their complexes with inter- leukin-19 and interleukin-20. Biochemistry 42, 12617– 12624. 43 Sheikh F, Baurin ... DNA binding, and compared the cDNAs of IFN-treated cells over- expressing intact STAT2 to those of IFN-treated cells overexpressing mutated STAT2 lacking the DNA binding domain...

Ngày tải lên: 19/02/2014, 07:20

... follows: aprotinin, leu- peptin, pepstatin A, AEBSF and proteinase inhibitor mixture that contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A from Wako Pure Chemical Industries (Osaka, ... 2006 FEBS 627 Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin...

Ngày tải lên: 19/02/2014, 07:20