Báo cáo khoa học: Tetranectin binds hepatocyte growth factor and tissue-type plasminogen activator potx

... Tetranectin binds hepatocyte growth factor and tissue-type plasminogen activator Uffe B. Westergaard, Mikkel H. Andersen, Christian W. Heegaard, Sergey N. Fedosov and Torben E. ... hepatocyte growth factor (HGF) and tissue-type plasminogen activator (tPA), whereas threerelated proteins: macrophage-stimulating protein (MSP),urokinase-type plasminogen activator (uPA), and ... found by ligand blotanalysis and ELISA that tetranectin speciﬁcally bound to the plasminogen- like hepatocyte growth factor and tissue-type plasminogen activator. The dissociation constants of thesecomplexes...

Báo cáo khoa học: Control of transforming growth factor b signal transduction by small GTPases pot

... Colicelli J (2008) Integration oftransforming growth factor beta and RAS signalingsilences a RAB5 guanine nucleotide exchange factor and enhances growth factor- directed cell migration.Mol Cell Biol ... PRK2 and p38 MAPK and the transcriptionalupregulation of smooth muscle-speciﬁc genes such asa-SMA, SM-MHC and SM-22a via the cooperation ofserum response factor, GATA and myosin enhancer factor ... kinase 1 and ROCK1 phosphorylate and activate LIM-kinases 1 and 2, respectively [128–132]. Eventually, LIM-kinases1 and 2 phosphorylate actin-depolymerizing proteinssuch as coﬁlin, destrin and actin-depolymerizing...

Báo cáo khoa học: Binase cleaves cellular noncoding RNAs and affects coding mRNAs potx

... intracellular RNA and the expression of proapoptotic and antiapoptotic mRNAs. For fourcell lines, we visualized cellular RNA by ﬂuorescence microscopy, and determined RNA levels, viability and apoptosis ... catalyzes RNA cleavage without the need formetal ions and cofactors. Cloning and sequencing ofthe binase gene have been reported [22], and a three-dimensional structure at 1.65 A˚resolution ... RNA fragmentsobserved after the digestion of a single-stranded and adouble-stranded hSK4 mRNA sample with binase, thenontoxic RNase Sa, and its highly cytotoxic cationicmutant 5K RNase Sa [20],...

Báo cáo khoa học: "A Comparison of Document, Sentence, and Term Event Spaces" potx

... ranked list (Luhn 1958). Figures 2D, E and F show the standard deviation be-tween the document and sentence space, the document and term space and the sentence and term space respectively. These ... sentence, and term, spaces in our corpora of more than one-hundred thousand full-text docu-ments; Section 4 discusses the results; and Sec-tion 5 draws conclusions from this study. 2 Background and ... for different sized corpora and vocabulary. Understanding the relationship among docu-ment, sentence and term spaces also has practical importance. The size and nature of text corpora has...

Báo cáo khoa học: Haptoglobin binds the antiatherogenic protein apolipoprotein E – impairment of apolipoprotein E stimulation of both lecithin:cholesterol acyltransferase activity and cholesterol uptake by hepatocytes pdf

... gel in denaturing and reducing condi-tions, and by western blotting. (A) Coomassie-stained bands ofisolated Hpt (lane 1), standard ApoE (lane 2), standard ApoA-I (lane3), and partially puriﬁed ... HRP-conjugated avidin and ECL. Coomassie-stained bands of VLDL and LDL proteins are shown in lanes 1 and 2, respectively. VLDL and LDL proteins, blotted onto the PVDFmembrane and incubated with ... IgG and rabbit anti-goat HRP-conjugated IgG. Two bands,reacting with the antibodies and with the molecularmasses of b and a2(41 and 21 kDa, respectively), weredetected (Fig. 4, lanes 5 and...

Báo cáo khoa học: The guanine nucleotide exchange factor RasGRF1 directly binds microtubules via DHPH2-mediated interaction docx

... recep-tors [20,21] and requires both calcium and calmodulinto exert its activity on Ras [18,21]. The protein is phos-phorylated on serine, threonine and tyrosine residuesin vivo and is a substrate ... 6). Supernatants and pellets were isolated by centrifugation, and equal volumes were used for SDS ⁄ PAGE and analyzed by immuno-blotting using monoclonal antibodies to a-tubulin and RasGRF1.Lane ... MT). Microtubules and associatedproteins were isolated and Input, supernatant and microtubulepellet (P) fractions were analyzed by western blotting using anti-bodies to RasGRF1 and a-tubulin.ABFig....

Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

... loss-of-functionof BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYKAlamdar Hussain1,2,*, Liang Yu1,3,*, Rani Faryal1,2, Dara K. Mohammad1, Abdalla J. Mohamed1,4 and C. I. Edvard ... COS7 and 293Tcells and did not vary like BTK–SYK; therefore, thedifferences in the PH–TH domains remain the decisive factor for this variation.The B-cell adapter protein BLNK (SLP-65) and itsT-cell ... our under-standing of signaling mechanisms in general.Mutation spectrum in XLA and genotype–phenotype correlationsFigure 1 depicts the linear organization of the domainsin BTK and Fig. 2 shows...

Tài liệu Báo cáo khoa học: a-enolase: a promising therapeutic and diagnostic tumor target ppt

... PAI-1, plasminogen activator inhibitor-1; PTM, post-translational modiﬁcation; TAA, tumor-associated antigen; tPA, tissue-type plasminogen activator; uPA, urokinase-type plasminogen activator; uPAR, ... levels of plasminogen activation and malignancy [46,52]. Higher expressionlevels of uPA and ⁄ or plasminogen activator inhibitor-1(PAI-1) in tumor tissues correlate with aggressiveness and poor ... cell-associated plasminogen by tissue-type plasminogen activator. J Biol Chem 271, 25684–25691.41 Borza DB & Morgan WT (1997) Acceleration of plas-minogen activation by tissue plasminogen activator...

Tài liệu Báo cáo khoa học: Mitochondrial chaperone tumour necrosis factor receptor-associated protein 1 protects cardiomyocytes from hypoxic injury by regulating mitochondrial permeability transition pore opening docx

... apoptosis and maintaining mitochon-drial homeostasis and function. Silencing TRAP1enhances cytochrome c release from the mitochondria and apoptosis induced by b-hydroxyisovalerylshikonin and VP16 ... understanding of the protectiverole of TRAP1 in cardiomyocytes and offer new consid-erations for myocardial protection after burn shock.Materials and methodsCardiomyocyte culture and hypoxia ... cell deaththat occurs during hypoxia and other conditions [9].Tumour necrosis factor receptor-associated protein 1(TRAP1) localizes to the mitochondria and its targetingsequence, which is...

Tài liệu Báo cáo khoa học: Paradoxical interactions between modiﬁers and elastase-2 Patricia Schenker and Antonio Baici docx

... poly-disperse, and the chondroitin sulfates exist as co-poly-mers of the 4- and 6-sulfate isomers (Ch4S, Ch6S) withvarious compositions and mean molecular masses thatdepend on animal species and tissue. ... described by Schechter and Berger [26],the four amino acids of H-TNVV-OMe bind at posi-tions S4-S3-S2-S1in the same order as written, i.e. T binds to S4 and so on, and eglin c is also ... modiﬁers and elastase-2 P. Schenker and A. Baici2492 FEBS Journal 277 (2010) 2486–2495 ª 2010 The Authors Journal compilation ª 2010 FEBSresidual activity using MeOSuc-AAPV-pNA. Inactivatorand...

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