Báo cáo khoa học: "A Novel Word Segmentation Approach for Written Languages with Word Boundary Markers" pptx
... 29–32, Suntec, Singapore, 4 August 2009. c 2009 ACL and AFNLP A Novel Word Segmentation Approach for Written Languages with Word Boundary Markers Han-Cheol Cho † , Do-Gil Lee § , Jung-Tae Lee § , ... work under the as- sumption that a user input is error-free; thus, word segmentation (WS) for written languages that use word boundary mark- ers (WBMs), such as...
Ngày tải lên: 17/03/2014, 02:20
... volume, 200 lL). The reactions were performed at 65 °C for 5 min without (solid line) or with (dotted line) 1 m M NADPH, and the products were then extracted with ice-cold acetonitrile (1.0 mL). ... were transformed with pET–FNR. The transformant was grown in 2 · YT medium containing chloramphenicol and ampicillin at 37 °CuptoanD 600 of 1.0, and FNR expression was induced by treatment...
Ngày tải lên: 18/02/2014, 08:20
... can provide stronger support for Chinese relation extraction. Meanwhile, it can be captured with less effort than applying deep natural language processing. But unfortunately, entity co-reference ... basic unit features within each feature subspace can already achieve state-of-art performance, while over-inclusion of complex features might hurt the performance. Previous approaches...
Ngày tải lên: 20/02/2014, 09:20
Báo cáo khoa học: A novel 2D-based approach to the discovery of candidate substrates for the metalloendopeptidase meprin pot
... ⁄ v) SDS, 50 mm Tris–HCl, pH 8.8, with 1% (w ⁄ v) dithioerythritol and 4.8% (w ⁄ v) iodoacetamide, respec- tively, with each step being performed for 15 min. For the second dimension, strips were ... Biosciences) with running conditions: 15 mA per gel for 1 h 30 min and 50 mA per gel for 5–6 h at 15 °C. For preparative gels, 1 mg of protein was solubilized in 2 mL of buffer f...
Ngày tải lên: 07/03/2014, 06:20
Tài liệu Báo cáo khoa học: A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and invasion docx
... fixed in 4% formaldehyde for 25 min at 4 °C and per- meabilized with 0.2% Triton X-100 for 5 min at room tem- perature. After being washed with NaCl ⁄ P i , coverslips were incubated with fluorescently ... MSP analysis for the occludin promoter and PCR with primers for exon 1 and exon 1a were performed using demethylating agent-treated cells. (C) Occ WT expression in Chang cells...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khoa học: A novel tachykinin-related peptide receptor of Octopus vulgaris – evolutionary aspects of invertebrate tachykinin and tachykinin-related peptide ppt
... the manufacturer’s instruction. A digoxigenin-labeled probe, DIG-TKRPR-5¢-2R, was used for Southern blotting. The method for detection was the same as those previously des- cribed [24]. As a negative ... as templates for PCR. Thus, we confirmed that there was no amplification of traces of the genomic DNA (data not shown). Expression of the cloned receptor in Xenopus oocytes The ORF region...
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khoa học: A novel dicyclodextrinyl diselenide compound with glutathione peroxidase activity ppt
... and the GPX activity for 6-CySeCD was higher than for 6-SeCD. Kinetics of 6-CySeCD Steady-state kinetics was observed for substrates H 2 O 2 and GSH. The initial velocities for reduction of H 2 O 2 by ... (b-CD) cavity provided a hydrophobic environ- ment for substrate binding [19–23]. For example, 6,6’-diseleno-bis-cyclodextrin (6-SeCD) activity for the reduction of hydrogen pe...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: A novel type of highly negatively charged lipooligosaccharide from Pseudomonas stutzeri OX1 possessing two 4,6-O-(1-carboxy)-ethylidene residues in the outer core region ppt
... supplemented with 10 m M malic acid as the sole carbon source, at 27 °C. For growth in liquid medium, 1 mL was inoculated with a single colony from a fresh plate, and grown for 18 h at 27 °C with constant ... alkaline treatment. As for the outer core structure of the LOS from P. stutzeri OX1, the structure we have determined was found to be of special interest, both for its nove...
Ngày tải lên: 19/02/2014, 13:20
Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
... was concentrated to 10 mL with a rotary evaporator. The pH of the concentrated solution was ad justed t o p H 3.0 with 5 M metaphosphoric acid, and th e s olution was extracted with ethyl acetate. ... were mixed with an equimolar concentration of pentafluorophenylhydrazine at 24 °C for 30 min. The reaction mixture was then evapor- ated to dryness. The hydrazone derivative was then mi...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: A novel c-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1 ppt
... into pH6EX3 digested with the same restriction enzymes. E. coli XL1-Blue, made transformation competent with the Roti- Transform kit (Roth, Karlsruhe, Germany), were trans- formed with the ligated ... revealed effective formation of the flavin-sulfite adduct (K D ¼ 150 l M ). Anaerobic titration with c-N-methylaminobuty- rate and sarcosine resulted in full reduction of the enzyme withou...
Ngày tải lên: 19/02/2014, 16:20