Báo cáo khoa học: "The Human Language Project: Building a Universal Corpus of the World’s Languages" pptx

Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

... ON357, 5¢-TGAAACATCACCAACTAAATCTCCAA-3¢; ON358, 5¢-GACGACTCCATTGTTATAGGAAAAGAGT-3¢; ON359, 5 ¢-GCCGCTGGAGAAACAGCAT-3¢; ON360, 5¢-GCCACCCATTCGTCACAATC-3¢;and ON361, 5¢-ATGCAGAAGGGGATCCGG-3¢.Direct ... in the supernatants were quanti-ﬁed by the BC assay quantiﬁcation kit (Uptima) using BSAas the standard. Equal amounts of protein were preparedfor separation by SDS ⁄ PAGE.ImmunoprecipitationHEK293 ... quantiﬁcation kit was from Uptima(Monticon, France). BAPTA-AM was from Calbiochem (LaJolla, CA, USA).Plasmids The pcDNA3.1(–) vector (Invitrogen), encoding the human 5-HT7 (a) receptor, was...

Tài liệu Báo cáo khoa học: "Exploiting Readymades in Linguistic Creativity: A System Demonstration of the Jigsaw Bard" docx

... combinations than the average human. The necessary barrage of linguistic stimulican be provided by the Google 1T database of Webngrams (Brants and Franz, 2006). Trawling thesengrams, a modestly ... orrestaurant names, or the names of racehorses, andso on, and on. The 1996 movie The Usual Suspectstakes its name from a memorable scene in 1942’sCasablanca, as does the Woody Allen play andmovie ... shall showthat the notion of a linguistic readymade has sig-nificant practical merit in the realms of text gen-eration and computational creativity.2 Linguistic ReadymadesReadymades are the...

Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-speciﬁc alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt

... 5¢-GGACTCAAGAGAGGAACCTG-3¢; F1b, 5¢-CTGCCTTGCTCCACACCTG-3¢; F1c, 5¢-CTGCCGTCTCCGCCGCCACT-3¢; FU, 5¢-TCAAGCCCCGCTACATAGTT-3¢; and R3, 5¢-AGGAACCAAACACCAAGTGG-3¢(Fig. 2A) .Luciferase promoter assay Human ... 5¢-TCATGCTGGTTGAGACTGGA-3¢ and 5¢-CCAGGTGTGGAGCAAGGCAG-3¢) and a 938 bp fragment of promoter 3 (primers, 5¢-CTCACAGCAGCCAGTAAGTG-3¢ and 5¢-ACTCACAGGCTCTGGGGCTG-3¢) were ampliﬁed using a Taq polymerase ... raised against KLK11 was the same sizeas that of the translational product by isoform 2mRNA (Fig. 4B). The product by isoform 1 mRNAwas smaller than the isoform 2 product. The multiplebands may...

Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc

... local Src activation triggered the long-range propa-gation of active Src wave into the distal cell areas awayfrom the site of mechanical stimulation [50]. The mecha-nism of this wave propagation ... À!kcat a1 S a1 þ S a1 S a2 þS Ðkf a2 kr a2 S a2 Á S À!kcat a2 S a2 þ S a1 ð1Þ The autophosphorylation rate (v3) is the sum of the rates catalyzed by each form. Applying quasi steady-state (QSS) approximation ... Cellular Systems ModellingDatabase and can be accessed at http://jjj.biochem.sun.ac.za/database/kaimachnikov/index.html free of charge.Calculation of the QSS curves and steady states The QSS...

Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc

... actin-binding domainThirumaran Thanabalu1,2, Rajamuthiah Rajmohan2, Lei Meng2, Gang Ren4,5, Parimala R. Vajjhala4and Alan L. Munn1,3,4,6*1 Institute of Molecular and Cell Biology, A* STAR Biomedical ... importance of WASP–WIP interaction in mammalian cells is sup-ported by the observation that the vast majority of the disease-causing missense mutations in human WASPmap to the N-terminal WH1 ... and growth atelevated temperature, on the one hand, and in corticalactin-patch polarization, on the other hand, are at leastpartially distinct [23]. However, there may still be a functional...

Tài liệu Báo cáo khóa học: Active-site residues and amino acid speciﬁcity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx

... 4¢-phosphopantothenate is not commercially available,it was synthesized enzymatically in situ by addingpantothenate kinase, His-CoaA,D-pantothenate andATP to the His-CoaB assay mixtures [5]. Therefore,0.9 ... with4¢-phosphopantothenoyl-CMP.Copuriﬁcation of the 4¢-phosphopantothenoyl-CMPintermediateTaking into account that residues Arg206, Asn210 andAla276 are crucial for the second-half reaction of the bacterial PPC synthetase activity, ... synthesis of b-alanine. Therefore it looks like, that an excess of D-pantothenate results in higher concentrations of 4¢-phos-phopantothenate and that the partial blocking of the Dfp(CoaBC)...

Tài liệu Báo cáo khoa học: What makes biochemical networks tick? A graphical tool for the identiﬁcation of oscillophores ppt

... removal. It is obtained for allsubstrates of any elementary reaction. The main point of the present section is that any Jacobianmatrix element equals the sum of a number of direct parallelinﬂuence ... inﬂuence paths) in the kineticscheme, i.e. the sum of paths through reaction-centeredgraphs of the type o f Graph 1 (these paths may containparallel and antiparallel arrows). The sign of that elementtherefore ... deﬁne the value of a subgraph that contains p reactions and p concentrations, asminus the sum of all its combined autoinﬂuence paths of order p. Note therefore that negativity of a subgraph andpositivity...

Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx

... CTCTTTGATGACAGAAGTGCCT-3¢Syndecan-4 5¢-85 GAGTCGATTCGAGAGACTGA-3¢ 3655¢-450 AAAAATGTTGCTGCCCTG-3¢Glypican-1 5¢-566 GAATGACTCGGAGCGTACACTG-3¢ 4885¢-1054 CCTTTGAGCACATTTCGGCAA-3¢P450 aromatase 5¢-1555GCTTCTCATCGCAGAGTATCCGG-3¢ ... 5¢-1555GCTTCTCATCGCAGAGTATCCGG-3¢ 2895¢-1821CAAGGGTAAATTCATTGGGCTTGG-3¢b-actin 5¢-2350 ACAGACTACCTCATGAAGAT-3¢ 6655¢-3222 AGCCATGCCAAATGTCTCAT-3¢Fig. 1. Dose-related eect of bFGF on FSH-stimulated ... USA).DNA quanti®cation The DNA content of the cell layer at the end of incubationwas quanti®ed by the method of West et al .[31].Aftersolubilization of the cell layer in 1MNaOH and s ubsequentneutralization...

Báo cáo khoa học: Structural and functional evidence for a singular repertoire of BMP receptor signal transducing proteins in the lophotrochozoan Crassostrea gigas suggests a shared ancestral BMP/activin pathway docx

... to the amino terminal end and C2deﬁnes the domain closest to the cell membrane. A comparison of the inferred amino acid sequence of the C1 and C2 domains reveals that only the approximatespacing ... tract, gills, heartand labial palp) including female gonads (oocytes),and from various stages of embryonic and larval devel-opment (blastula, gastrula, trochophore larvae, D lar-vae, 7 and ... using the minimum evolution method of the PAUP package. The percentage recovery of the branch in 1000 bootstrap replications is indicated. ActR2b Carassius auratus(ABB58749)Daf-1 Caenohabditis...

Báo cáo khoa học: Relation between domain evolution, speciﬁcity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot

... Slovak Academy of Sciences, Bratislava, Slovakia;2Department of Chemistry,Carlsberg Laboratory, Copenhagen Valby, Denmark;3Department of Chemistry, University of Manitoba, Winnipeg, Canada The ... in the amylase group of the SBD tree that couldinvolve also the cluster of acarviose transferase (Actsp),archaeal CGTase (Thcsp) and the maltogenic a- amylase(Bacst) due to its longer branch ... (SBD): a- amylase,maltotetraohydrolase, maltopentaohydrolase, maltogenic a- amylase, acarviose transferase, and cyclodextrin glu-canotransferase (CGTase). Such enzymes are multidomainproteins and...

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