... ON357, 5¢-TGAAACATCACCAACTAAATC TCCAA-3¢; ON358, 5¢-GACGACTCCATTGTTATAGG AAAAGAGT-3¢; ON359, 5 ¢-GCCGCTGGAGAAACAG CAT-3¢; ON360, 5¢-GCCACCCATTCGTCACAATC-3¢; and ON361, 5¢-ATGCAGAAGGGGATCCGG-3¢. Direct ... in the supernatants were quanti- fied by the BC assay quantification kit (Uptima) using BSA as the standard. Equal amounts of protein were prepared for separation by SDS ⁄ PAGE. Immuno...
Ngày tải lên: 19/02/2014, 18:20
... combinations than the average human. The necessary barrage of linguistic stimuli can be provided by the Google 1T database of Web ngrams (Brants and Franz, 2006). Trawling these ngrams, a modestly ... or restaurant names, or the names of racehorses, and so on, and on. The 1996 movie The Usual Suspects takes its name from a memorable scene in 1942’s Casablanca, as does...
Ngày tải lên: 20/02/2014, 05:20
Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt
... 5¢-GGACTCAAGAGAGGAACCTG-3¢; F1b, 5¢-CT GCCTTGCTCCACACCTG-3¢; F1c, 5¢-CTGCCGTCTCC GCCGCCACT-3¢; FU, 5¢-TCAAGCCCCGCTACATAG TT-3¢; and R3, 5¢-AGGAACCAAACACCAAGTGG-3¢ (Fig. 2A) . Luciferase promoter assay Human ... 5¢-TCATGCTGGTTGAGACTG GA-3¢ and 5¢-CCAGGTGTGGAGCAAGGCAG-3¢) and a 938 bp fragment of promoter 3 (primers, 5¢-CTCACAG CAGCCAGTAAGTG-3¢ and 5¢-ACTCACAGGCTCTGG GGCTG-3¢) were amplifi...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc
... local Src activation triggered the long-range propa- gation of active Src wave into the distal cell areas away from the site of mechanical stimulation [50]. The mecha- nism of this wave propagation ... À! k cat a1 S a1 þ S a1 S a2 þS Ð k f a2 k r a2 S a2 Á S À! k cat a2 S a2 þ S a1 ð1Þ The autophosphorylation rate (v 3 ) is the sum of the rates cataly...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc
... actin-binding domain Thirumaran Thanabalu 1,2 , Rajamuthiah Rajmohan 2 , Lei Meng 2 , Gang Ren 4,5 , Parimala R. Vajjhala 4 and Alan L. Munn 1,3,4,6 * 1 Institute of Molecular and Cell Biology, A* STAR Biomedical ... importance of WASP–WIP interaction in mammalian cells is sup- ported by the observation that the vast majority of the disease-causing missense mutations in human W...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khóa học: Active-site residues and amino acid specificity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx
... 4¢-phosphopantothenate is not commercially available, it was synthesized enzymatically in situ by adding pantothenate kinase, His-CoaA, D -pantothenate and ATP to the His-CoaB assay mixtures [5]. Therefore, 0.9 ... with 4¢-phosphopantothenoyl-CMP. Copurification of the 4¢-phosphopantothenoyl-CMP intermediate Taking into account that residues Arg206, Asn210 and Ala276 are crucial for the...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: What makes biochemical networks tick? A graphical tool for the identification of oscillophores ppt
... removal. It is obtained for all substrates of any elementary reaction. The main point of the present section is that any Jacobian matrix element equals the sum of a number of direct parallel influence ... influence paths) in the kinetic scheme, i.e. the sum of paths through reaction-centered graphs of the type o f Graph 1 (these paths may contain parallel and antiparal...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx
... CTCTTTGATGACAGAAGTGCCT-3¢ Syndecan-4 5¢-85 GAGTCGATTCGAGAGACTGA-3¢ 365 5¢-450 AAAAATGTTGCTGCCCTG-3¢ Glypican-1 5¢-566 GAATGACTCGGAGCGTACACTG-3¢ 488 5¢-1054 CCTTTGAGCACATTTCGGCAA-3¢ P450 aromatase 5¢-1555GCTTCTCATCGCAGAGTATCCGG-3¢ ... 5¢-1555GCTTCTCATCGCAGAGTATCCGG-3¢ 289 5¢-1821CAAGGGTAAATTCATTGGGCTTGG-3¢ b-actin 5¢-2350 ACAGACTACCTCATGAAGAT-3¢ 665 5¢-3222 AGCCATGCCAAATGTCTCAT-3¢ Fig. 1. Dose...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Structural and functional evidence for a singular repertoire of BMP receptor signal transducing proteins in the lophotrochozoan Crassostrea gigas suggests a shared ancestral BMP/activin pathway docx
... to the amino terminal end and C2 defines the domain closest to the cell membrane. A comparison of the inferred amino acid sequence of the C1 and C2 domains reveals that only the approximate spacing ... tract, gills, heart and labial palp) including female gonads (oocytes), and from various stages of embryonic and larval devel- opment (blastula, gastrula, trochophore larvae,...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Relation between domain evolution, specificity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot
... Slovak Academy of Sciences, Bratislava, Slovakia; 2 Department of Chemistry, Carlsberg Laboratory, Copenhagen Valby, Denmark; 3 Department of Chemistry, University of Manitoba, Winnipeg, Canada The ... in the amylase group of the SBD tree that could involve also the cluster of acarviose transferase (Actsp), archaeal CGTase (Thcsp) and the maltogenic a- amylase (Bacst) d...
Ngày tải lên: 08/03/2014, 08:20