Báo cáo khoa học: Secretion of the mammalian Sec14p-like phosphoinositide-binding p45 protein pptx

... vesicles and therefore cannot colocalize with p45. Fig. 3. Secretion of the SEC14 domain of p45. (A) The hydrophilicity plot of p45 protein and location of the SEC14 and GOLD domains. Note that the strongly ... Merkulova et al. Secretion of p45 Sec14p-like protein FEBS Journal 272 (2005) 5595–5605 ª 2005 FEBS 5605 Secretion of the mammalian Sec14p-l...

Ngày tải lên: 16/03/2014, 23:20

... colonizes the surface area of the gastric mucosa in the human stomach and is one of the most adapted microbial pathogens.  50% of the world’s population carries this bacterium, causing chronic, often ... formation of VirB2-VirB5 complexes followed by T-pilus assembly. The assem- bled T4SS then triggers the secretion of substrates from the bacterial cytoplasm direc...

Ngày tải lên: 14/02/2014, 19:20

... post-translational modifications HSF1 is the prototype of all HSFs and the mammalian counterpart of the single HSF of yeasts, nematodes and fruit flies [3,27–29]. Deletion experiments of the Drosophila Hsf demonstrated ... induce the synthesis of HSPs that act as molecular chaperones through binding to the hydrophobic surfaces of unfolded proteins, thereby facilitating...

Ngày tải lên: 18/02/2014, 04:20

... between the levels of expres- sion in the different experiments (Table 2). The results show a clear relationship between the thermal stability of each variant and the level of protein secreted to the supernatant ... both variants suggesting that the overall structure of the folded proteins is unlikely to differ significantly from that of the WT protein. The for...

Ngày tải lên: 19/02/2014, 08:20

... proteins. Other components are essential for the formation of the T4SS complex: VirB1 allows for the insertion of the system in the periplasm and VirB3, the function of which is unknown, is often associated ... helices from each of the monomers pointing into the centre of the ring to form the claws of the grapple [28]. VirB11 proteins are dynamic assemblies,...

Ngày tải lên: 06/03/2014, 00:21

... binding of anesthetic to the four-a-helix bundle scaffolds is associ- ated with a stabilization of the folded conformation of the protein. Halothane has been shown to increase the stability of the ... (http://www.microcal.com/). This software provides the best-fit values for the heat of binding (DH°), the stoichiometry of binding (n), and the association constant...

Ngày tải lên: 07/03/2014, 16:20

... suffered at least loss of one rel- ative, for example, a partner or child, and two mothers of our cohort lost both of their children. The majority of patients com- plained of nightmares, emotional ... aftermath With respect to the tsunami's psychoemotional aftermath, the full impact of the wave on the mental health of the survivors is still unknown [2]. In Febru...

Ngày tải lên: 25/10/2012, 10:41

... as the ATP-unbound and Ca 2+ -bound E1Ca 2+ form. In the histogram (Fig. 2A) of the dis- tribution of the height of the projection of the embed- ded molecule above the flat membrane surface, the average ... Histograms of the height differences between the top of SERCA and the surface of the membrane. Statistical section analy- ses of SERCA were performed...

Ngày tải lên: 14/02/2014, 14:20

... for a therapeutic approach in AD [13–15]. Protein structure and gene organization of ADAM10 The enzyme ADAM10 belongs to the subgroup of metzincins within the zinc proteinases family. The typi- cal ... that the prodomain of ADAM10 acts not only as a transient inhibitor, but also as an internal chaperone in the mat- uration of the enzyme. Accordingly, the viral delive...

Ngày tải lên: 16/02/2014, 09:20

... preferentially the phosphorylation of the p46 isoform of JNK, whereas sorbitol treatment induced phosphorylation of both the p46 and p55 isoforms. To confirm functional activation of the JNK signaling ... phosphorylation of the transcription factor c-JUN was attenuated. Upon expression of wild-type JSP1, a subpopula- tion of cells, with the highest levels of the p...

Ngày tải lên: 16/02/2014, 14:20