Báo cáo khoa học: Differential expression pattern of the novel serine⁄threonine kinase, STK33, in mice and men doc

... expression pattern A. O. Mujica et al. 4898 FEBS Journal 272 (2005) 4884–4898 ª 2005 FEBS Differential expression pattern of the novel serine⁄ threonine kinase, STK33, in mice and men Alejandro O. ... for the kinase domain including the peptide used for immunization and is in frame with the start and stop codons of the PinPointä Xa)1T (Promega, Madi...

Ngày tải lên: 16/03/2014, 23:20

... located in the sub- unit contact area of the tetramer, whereas the remaining two mismatches sur- round the contact area; this is expected to manifest in the kinetic and thermodynamic properties of the ... different kinetic and thermodynamic properties of the tetrameric enzyme may result in fine-tuning of the properties of skeletal LDH-A, which is in line w...

Ngày tải lên: 19/02/2014, 02:20

... molecular mass of 47 kDa in both monocytes and macrophages (Fig. 4B), with an increase in intensity of staining of this protein in macrophages (Fig. 4B). Thus, these results suggest that the absolute ... discrepancy between amount of RNA and enzyme activity was likely not due to changes in the expression of cathepsin A, as the specific activity of cathepsin A in...

Ngày tải lên: 16/03/2014, 19:20

... overexpression of enolase 1 and malic enzyme 1 supports the induction of fatty acid metabolism. The reduction in d-amino acid oxi- dase 1 expression in the kidney proteome is interesting, in view ... detected in knock- out mice. In 14 of the 18 spots, MALDI peptide mass fingerprints allowed the identification of the correspond- ing proteins in the database...

Ngày tải lên: 23/03/2014, 03:20

... multiple endo- crine neoplasia (MEN) syndromes (MEN2 a and MEN2 b), as well as in agangliosis of the colon, one of the most common developmental defects in young chil- dren [1]. In the developing nervous ... results in a loss of both Frs2- and Shc-induced increases of Ret in the DRM fraction. Analysis of the DRM and SUP fractions suggested that both Shc a...

Ngày tải lên: 07/03/2014, 05:20

... as indicated, together with a luciferase reporter containing two copies of the Hox ARE r3 element, which binds Hox and Pbx proteins. (E) The effect of expressing increasing amounts of either the ... pro- moting cooperative binding of Meis–Pbx dimers to a composite DNA element [34,35]. The interaction of the Meis and Pbx partners also facilitates binding of the Pbx...

Ngày tải lên: 16/02/2014, 15:20

... potential to interfere with the biological activity of IFN-c. In the SW480 cell line, IFN-c treatment resulted in the inhibition of the STAT3-dependent cyclin D1 promoter, and activation of the STAT1- 2000 2500 IRF1 Luciferase ... (particularly in whole ani- mals) by impairing the action of STAT1-dependent interferon. The results obtained in the present study...

Ngày tải lên: 18/02/2014, 08:20

... found in the 23S rRNA variant containing the double mutation U1911C ⁄ U1915C (Fig. 3). Thus, isomerization of uri- dines 1911 and 1917 in H69 occurs autonomously of each other and is independent of ... in the presence of 10 mm arabinose and made competent by con- centrating ten-fold and washing five times with ice-cold 10% glycerol. Selection for the recombination...

Ngày tải lên: 18/02/2014, 16:20

... report the binding potentials between the phenol-hydro- xyl group of bisphenol A and estrogen-related receptor c residues Glu275 and Arg316 in the ligand-binding domain. By inducing mutations in other amino ... activity of the latter and to reverse the deactivating inverse agonist activity of 4-hydroxytamoxifen. However, the intrinsic binding mode of bisphenol A...

Ngày tải lên: 18/02/2014, 16:20

... ‘remote’ attachment of residues 35–81 from its own C-terminal segment, cause only minor perturbation of the inhibi- tory potency intrinsic to the N-terminal segment. The effect of extending the N-terminal ... by determination of inhibition constants for the interaction of the N-terminally exten- ded inhibitors with saccharopepsin. The potencies of inhibitors 9 (conta...

Ngày tải lên: 19/02/2014, 00:20