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Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx

Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx

Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx

... oyster CaM and CaLP. In the presence of calcium, oyster CaLP and CaM appeared as a single band with an apparentmolecular weight of approximately 18 kDa and 14 kDa, respectively, whereas in the absence ... oyster calcium metabolism and calcium signaling pathways.Experimental proceduresRNA preparation and cDNA synthesisAdult specimens of P. fucata were purchased from Guofa Pearl Farm, Beihai, Guangxi ... architectures and outstandingmechanical performance, have received a great deal of attention from many biology and materials scientistsin the past few decades [1]. Shells and pearls are allproducts of...
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Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot

Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot

... been identified and can be divided intoCHH -A and CHH-B groups [19]. In the crabs Can-cer pagurus, Carcinus maenas and Libinia emarginata and in the crayfishes Procambarus clarkii and Orconec-tes ... sequence, and 2.5 units of Taq DNA polymerase(Heber Biotec S .A. , Havana, Cuba). The amplification of CHH cDNA was carried out in 30 cycles as follows: denatur-ation 30 s at 95 °C, 1 min annealing at ... analysis comparison. (A) Sequence analysis comparison by CLUSTALW analysis among CHH cDNA reported forMarsupenaeus japonicus (AB035724), Metapenaeus ensis (AF109775), Litopeneaus vannamei (AY434016),...
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Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf

Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf

... (TaKaRa Bio Inc.). Forward primers 5¢-CGTTTGAAGGGTGAGGAGGAAAA[FAM]G-3¢ and 5¢-CACAAGAGAGTTCTGCGATAACCTTG[FAM]G-3¢ (Invi-trogen Corporation) and reverse primers 5¢-AAGTAGGCAACAAAACAACG-3¢ and ... 77–99.16 Kamauchi S, Wadahama H, Iwasaki K, Nakamoto Y,Nishizawa K, Ishimoto M, Kawada T & Urade R (2008)Molecular cloning and characterization of two soybean protein disulfide isomerases as molecular ... 4141Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassicactive center motifsKensuke Iwasaki1, Shinya Kamauchi1,*, Hiroyuki Wadahama1, Masao...
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Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulfide isomerases as molecular chaperones for seed storage proteins doc

Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulfide isomerases as molecular chaperones for seed storage proteins doc

... GmPDIL-1 and GmPDIL-2 by PCR using theoligonucleotide primers 5¢-GACGACGACAAGATGGAGGAATCATCGGAGAAAGAGTTC-3¢ and 5¢-GAGGAGAAGCCCGGTTCAAAGCTCATCTTTTCCTTTTTC-3¢ forGmPDIL-1, and 5¢-GACGACGACAAGATGCTCACCGACGACGAGGACC-3¢ ... 5¢-GTCTGTGGTACCTCCTTCAAAACCCCCTCCT-3¢ and 5¢-CGTGATGGTACCGGGTGTGCAACCCACATGTA-3¢ for GmPDIL-1, and 5¢-CAGCCCGCAGTTGAAAGTCAACCAAGTC-3¢ and Oligo dT-Adaptor primer FB (TaKaRa Bio Inc.) for GmPDIL-2.Amplicons ... oligonucleotideprimers 5¢-CCCAATTTGGAAGCTGATCACAT-3¢ and 5¢-CTTCCTTGGTCCTACCCCCTTCGT-3¢ for GmPDIL-1, and 5¢-AGCCCGAGGTGGACGAGAAGG-3¢ and 5¢-TTTGCCACATCAGGATCCACAGTTT-3¢ for GmPDIL-2, and sequencing.His-tagged expression...
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Báo cáo khoa học: Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica doc

Báo cáo khoa học: Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica doc

... 5¢-RACE,5¢-GSP1 (5¢-GTTATGGTGTAAGGCATAAAGATTAACCATAACC-3¢) and 5¢-GSP1 nested (5¢-TGTAAGGCATAAAGATTAACCATAACCCTAGTACC-3¢) and 3¢-RACE, 3¢-GSP1 (5¢-AATAAGGGTACTAGGGTTATGGTTAATCTTTATGC-3¢) and ... forward primer (5¢-TTTCCCAAAAGAAACTAGTAATGG-3¢) and reverse primer(5¢-ATACTAGTGCACGGTTAAGTGATTAATTACG-3¢)for CYP71 9A2 , and the forward primer (5¢-ATACTAGTATGGAGGAGATGAAGTTCTTGATAATG-3¢)andreverseprimer ... reverse GAAAGAAACAGAGCAAATCTTATCCTTTTACCCYP71 9A3 forward CCTCGTAACTAATATACCAGTGTGGTGCYP71 9A3 reverse GACAACCAAGCAAACTCTTATTCTTGTACInternal control geneb-actin forwardGGTATTGTGCTGGATTCTGGTGb-actin...
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Báo cáo khoa học: ˚ cDNA cloning and 1.75 A crystal structure determination of PPL2, an endochitinase and N-acetylglucosaminebinding hemagglutinin from Parkia platycephala seeds potx

Báo cáo khoa học: ˚ cDNA cloning and 1.75 A crystal structure determination of PPL2, an endochitinase and N-acetylglucosaminebinding hemagglutinin from Parkia platycephala seeds potx

... Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil8 Faculdade de Medicina, Universidade Federal do Ceara´, Sobral, Brazil9 Laboratorio de Bioquı´mica Marinha, Departamento ... Mimosoideae thathave been functionally and structurally characterizedare those from seeds of species of the genus Parkia,including Parkia speciosa [14], Parkia javanica [15],Parkia discolor [16] and ... comprising about 30 species found inthe neotropics from Honduras to south-eastern Brazil,West Africa, the northern part of Malaysia and thesouth of Thailand. Parkia platycephala is an importantforage...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crabParatelphusa jacquemontiiMaghil Denis, P. D. Mercy Palatty, N. Renuka Bai and S. Jeya SuriyaDepartment ... sialidasetypeX,proteaseenzymes and molecular mass standards were purchased from Sigma.Preparation of crab seraFreshwater field crabs, Paratelphusa jacquemontii werecollected from the local ... Denmark.47. Kamiya, H. & Ogata, K. (1982) Hemagglutinins in the acornbarnacle Balanus (Megabalanus roseus): purification and char-acterization. Nippon Suisan Gokkaishi 48, 1427.48. Kamiya,...
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Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf

Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf

... h as an a 2b2structure and containssiroheme, nonheme iron, and acid-labile sulfur [36]. Anadditional protein with an apparent molecular mass of 11 k Da is associated with sulfite reductase ... including AF499,have an N-terminal Ôtwin-arginineÕ signal sequence that ischaracteristic of cofactor-containing proteins translocatedinto the periplasm via the Tat translocase [27]. As deduced from ... of AF499 was identified asan archaeal promoter element by seque nce analysis. The sequ ence AAAGGTTAATATA shows a high le vel of identity with th e consensus se quence()35 to )23, AAANNNTTATATA)...
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Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

... bp wasproduced by PCR amplification with TOPO 2.1-SmNR1 as a template (forward primer: 5¢-ATTTCAGAAGTTGAACAAACACAC-3¢, reverse primer: 5¢-AAGATGGTATTGAAGATGATGGTTGA-3¢), purified from agarose ... 5¢-CCGTAAGGTCACAGGTCACTCG-3¢, DR2:5¢-CCGTAAGGTCACAAGGTCACTCG-3¢,DR3:5¢-CCGTAAGGTCACAGAGGTCACTCG-3¢, DR4: 5¢-CCGTAAGGTCACAGGAGGTCACTCG-3¢, DR5: 5¢-CCGTAAGGTCACCAGGAGGTCACTCG-3¢. PAL0: 5¢-CGCAAGGTCATGACCTCG-3¢. ... (ACGCTCACTGGAACACTGGAATGCCCAGTTCTCGTCGCTCACTGGAACACTGGAATGCCCAGTTCTCGTTCGCTCACTGGAACACTGGAATGCCTCTAG) upstream of the thymidine-kinasepromoter of reporter plasmid pUTK-Luc vector, whichcontains...
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Báo cáo khoa học: Identification and characterization of four novel peptide motifs that recognize distinct regions of the transcription factor CP2 doc

Báo cáo khoa học: Identification and characterization of four novel peptide motifs that recognize distinct regions of the transcription factor CP2 doc

... (5¢-GAAACCATTTTGCAGCGAAAGTATACAC-3¢) forPro ⁄ Arg to Ala ⁄ Ala mutations in bases 803–830. The twooverlapping PCR fragments were mixed and added as a template in the second PCR that used 1–19 and ... Wilanowski T, Cerruti L, Zhao LL, Cunning-ham JM & Jane SM (2003) The identification and char-acterization of human Sister -of- Mammalian Grainyhead(SOM) expands the grainyhead-like family of ... Tothese extracts (200 lg of protein) , appropriate antibodies and 10 lL of protein A Sepharose beads (Sigma-Aldrich)were added. After incubation for 1 h at room temperature,the beads were washed three...
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