Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx
Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx
... oyster CaM and CaLP. In the presence of calcium, oyster CaLP and CaM appeared as a single band with an apparent molecular weight of approximately 18 kDa and 14 kDa, respectively, whereas in the absence ... oyster calcium metabolism and calcium signaling pathways. Experimental procedures RNA preparation and cDNA synthesis Adult specimens of P. fucata were purchased...
Ngày tải lên: 16/03/2014, 23:20
Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot
... been identified and can be divided into CHH -A and CHH-B groups [19]. In the crabs Can- cer pagurus, Carcinus maenas and Libinia emarginata and in the crayfishes Procambarus clarkii and Orconec- tes ... sequence, and 2.5 units of Taq DNA polymerase (Heber Biotec S .A. , Havana, Cuba). The amplification of CHH cDNA was carried out in 30 cycles as follows: denatur- ation 30 s at...
Ngày tải lên: 23/03/2014, 10:20
Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf
... (TaKaRa Bio Inc.). Forward primers 5¢-CG TTTGAAGGGTGAGGAGGAAAA[FAM]G-3¢ and 5¢-CA CAAGAGAGTTCTGCGATAACCTTG[FAM]G-3¢ (Invi- trogen Corporation) and reverse primers 5¢-AAGTAGGCA ACAAAACAACG-3¢ and ... 77–99. 16 Kamauchi S, Wadahama H, Iwasaki K, Nakamoto Y, Nishizawa K, Ishimoto M, Kawada T & Urade R (2008) Molecular cloning and characterization of two soybean protein disulfi...
Ngày tải lên: 18/02/2014, 11:20
Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulfide isomerases as molecular chaperones for seed storage proteins doc
... GmPDIL-1 and GmPDIL-2 by PCR using the oligonucleotide primers 5¢-GACGACGACAAGATGGAG GAATCATCGGAGAAAGAGTTC-3¢ and 5¢-GAGGAGA AGCCCGGTTCAAAGCTCATCTTTTCCTTTTTC-3¢ for GmPDIL-1, and 5¢-GACGACGACAAGATGCTCACCGA CGACGAGGACC-3¢ ... 5¢-GTCTGTGGTACCTCCTTCAAAA CCCCCTCCT-3¢ and 5¢-CGTGATGGTACCGGGTGTG CAACCCACATGTA-3¢ for GmPDIL-1, and 5¢-CAGCCC GCAGTTGAAAGTCAACCAAGTC-3¢ and Oligo dT- Adaptor...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica doc
... 5¢-RACE, 5¢-GSP1 (5¢-GTTATGGTGTAAGGCATAAAGATTA ACCATAACC-3¢) and 5¢-GSP1 nested (5¢-TGTAAGGCA TAAAGATTAACCATAACCCTAGTACC-3¢) and 3¢- RACE, 3¢-GSP1 (5¢-AATAAGGGTACTAGGGTTATGG TTAATCTTTATGC-3¢) and ... forward primer (5¢-TTTCC CAAAAGAA ACTAGTAATGG-3¢) and reverse primer (5¢-ATACTAGTGCACGGTTAAGTGATTAATTACG-3¢) for CYP71 9A2 , and the forward primer (5¢-ATACTAGTA TGGAGGAGATGAAGTTCTTGATA...
Ngày tải lên: 07/03/2014, 10:20
Báo cáo khoa học: ˚ cDNA cloning and 1.75 A crystal structure determination of PPL2, an endochitinase and N-acetylglucosaminebinding hemagglutinin from Parkia platycephala seeds potx
... Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil 8 Faculdade de Medicina, Universidade Federal do Ceara ´ , Sobral, Brazil 9 Laboratorio de Bioquı ´ mica Marinha, Departamento ... Mimosoideae that have been functionally and structurally characterized are those from seeds of species of the genus Parkia, including Parkia speciosa [14], Parkia javanica [15], Pa...
Ngày tải lên: 16/03/2014, 13:20
Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii Maghil Denis, P. D. Mercy Palatty, N. Renuka Bai and S. Jeya Suriya Department ... sialidasetypeX,protease enzymes and molecular mass standards were purchased from Sigma. Preparation of crab sera Freshwater field crabs, Paratelphusa jacquemont...
Ngày tải lên: 21/02/2014, 00:20
Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf
... h as an a 2 b 2 structure and contains siroheme, nonheme iron, and acid-labile sulfur [36]. An additional protein with an apparent molecular mass of 11 k Da is associated with sulfite reductase ... including AF499, have an N-terminal Ôtwin-arginineÕ signal sequence that is characteristic of cofactor-containing proteins translocated into the periplasm via the Tat translocase [27]. A...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
... bp was produced by PCR amplification with TOPO 2.1-SmNR1 as a template (forward primer: 5¢-ATTTCAGAAGTTGAAC AAACACAC-3¢, reverse primer: 5¢-AAGATGGTATT GAAGATGATGGTTGA-3¢), purified from agarose ... 5¢-CCGTAAGGTCACAGGTCACTCG-3¢, DR2: 5¢-CCGTAAGGTCACAAGGTCACTCG-3¢,DR3:5¢-CCG TAAGGTCACAGAGGTCACTCG-3¢, DR4: 5¢-CCGTAA GGTCACAGGAGGTCACTCG-3¢, DR5: 5¢-CCGTAAGG TCACCAGGAGGTCACTCG-3¢. PAL0: 5¢-CGCA...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Identification and characterization of four novel peptide motifs that recognize distinct regions of the transcription factor CP2 doc
... (5¢-GAAACCATTTTGCAGCGAAAGTATACAC-3¢) for Pro ⁄ Arg to Ala ⁄ Ala mutations in bases 803–830. The two overlapping PCR fragments were mixed and added as a template in the second PCR that used 1–19 and ... Wilanowski T, Cerruti L, Zhao LL, Cunning- ham JM & Jane SM (2003) The identification and char- acterization of human Sister -of- Mammalian Grainyhead (SOM) expands the grainyhead-...
Ngày tải lên: 16/03/2014, 18:20