Báo cáo khoa học: The first cytochrome P450 in ferns Evidence for its involvement in phytoecdysteroid biosynthesis in Polypodium vulgare doc

... of the res- idues involved in phosphate and calcium binding are conserved in the PhyH-DII sequence, whereas only one phosphate-binding residue is conserved in the PhyH-DI sequence (Fig. S1). There ... contained 0–5.0 mm CaCl 2 . The optimal pH for enzyme activity was determined in the presence of the opti- mal Ca 2+ concentration at 37 °C for 30 min with the fol- low...

Ngày tải lên: 14/02/2014, 15:20

... reflection in the positive mode. Determination of the logP values for ginkgotoxin and PL The determination of the logP values was performed according to OECD guidelines for the testing of chemicals, partition ... comparison of the vitamin B 6 concentration in human blood and the gin- kgotoxin concentration in human blood after ingestion of Ginkgo remedies. The maximum...

Ngày tải lên: 07/03/2014, 10:20

... highlighted the role of Glu in the quinone/quinol site constitution. As the selected NrfH segment provides several amino acids fulfilling the above description, we propose that the putative quinone-binding ... proteins containing six c-type heme prosthetic groups, as judged by pyridine hemochrome assays and iron content determinations using the mature protein. However, in 1993,...

Ngày tải lên: 21/02/2014, 00:20

... reflection of the more basic f-amino group of the coordinating K100, with the H-bonded water molecule found in the X-ray structure increasing the electron donating power of the coordinating amino group ... different. In the wt structure the side chain slithers along the side of the protein surface, whereas in the M100K structure it points out into the solvent (Fig...

Ngày tải lên: 07/03/2014, 17:20

... bovine P450scc after introduction of a 25-hydroxyl group into the cholesterol side chain [20,24]. Since 25-hydroxylation of vitamin D3 is a limiting step in its activation, the latter finding ... (Fig. 5). The significant accumulation of 20(S)-hydroxyvitamin D3 (Fig. 1A) indicates easy release from the active site of the enzyme with only a minor portion remaining (or rebinding) f...

Ngày tải lên: 07/03/2014, 21:20

... decision making in the ICU. In that study, a questionnaire was com- pleted for each radiograph, addressing the indication for the radiograph and whether it changed the patient's management. Of the ... 'old expected finding' was any old finding predicted by the attending physician; a 'new unex- pected finding' was any new finding not predicted by t...

Ngày tải lên: 25/10/2012, 10:39

... alone. The kinase-deficient mutant, OXI1 K45R , in which the lysine residue of the ATP bind- ing domain is mutated to arginine, still interacted with PTI1-4. These data indicate that the kinase ... of the input was loaded on to the gel and represents the amount of HIS-tagged proteins used for the assay. The in vitro binding assays were repeated twice using recombinant p...

Ngày tải lên: 14/02/2014, 19:20

... confirmed by Lin-54 bind- ing to the CHR, cooperating with E2F4 binding to the CDE in EMSAs in vitro [86]. ChIP experiments on the Cdc2 gene also provided insights into the association of other cell ... proteins with the promoter. E2F4 binding during the cell cycle coincides with the binding of p107 or p130 [61,85]. Another E2F site distal to the E2F ⁄ CDE acts as an activa...

Ngày tải lên: 16/02/2014, 09:20

... [12]. Maintaining the balance between cell survival and apoptosis is critical in the maintenance of a healthy organism, and tipping the equilibrium in one or another direction results in either Keywords apoptosis; ... [16,17]. The BH3 domain-only proteins are a subfamily of the Bcl-2 family involved in the initiation of apoptosis through the mitochondrial pathway. The...

Ngày tải lên: 16/02/2014, 09:20

... were 231 min for the b-globin PAI)2 wild-type transcript, 204 min f or the b-globin ARE I-MUT , 193 min for the b-globin ARE III-MUT and 224 min for the b-globin ARE I+III-MUT . These experiments ... investigate the means by which these ARE elements cooperate in modulating PAI-2 mRNA stability, REMSA analyses were performed to determine the role of the ARE II and ARE...

Ngày tải lên: 16/02/2014, 09:20