Báo cáo khoa học: Coenzyme A affects ﬁreﬂy luciferase luminescence because it acts as a substrate and not as an allosteric effector pot

... A affects ﬁreﬂy luciferase luminescence because it acts as a substrate and not as an allosteric effector Hugo Fraga1,2, Diogo Fernandes1,2, Rui Fontes2 and Joaquim C. G. Esteves da Silva11 ... structural similarity between ﬁreﬂy luciferase and acyl-CoA synthetases, our achievement is not as strange as it seems to be and supports the theory thatnowadays ﬁreﬂy luciferase evolved from an ancestralacyl-CoA ... dinucleosidepolyphosphates catalyzed by ﬁreﬂy luciferase and several ligases. Pharmacol Ther 87, 91–102.16 Nakamura M, Maki S, Amano Y, Ohkita Y, NiwaK, Hirano T, Ohmiya Y & Niwa H (2005) Fireﬂyluciferase...

Báo cáo khoa học: New application of ﬁreﬂy luciferase ) it can catalyze the enantioselective thioester formation of 2-arylpropanoic acid docx

... basicregion.Thioesteriﬁcation acitivity of L. cruciata and P. pyralis luciferasesFrom the above experiments (Table 1, entries 1–3), it was known that L. cruciata and P. pyralis luciferasesalso have an ability to catalyze ... also has the ability to formthioesters of long-chain fatty acids. This report indica-ted that ﬁreﬂy luciferase also has an acyl-CoA synthe-tase activity toward an unnatural substrate. Inaddition, ... pyralis and recombinant luciferase from L. cruciata were purchasedfrom Sigma (St Louis, MO, USA) and Wako (Osaka,Japan), respectively. Thermostable luciferase fromL. lateralis (LUC-H) was thankfully...

Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx

... TnrA and disable its activity. In cells grown with an energetically unfavorable nitrogen source such as nitrate, TnrA is fullymembrane-bound via a complex of AmtB and GlnK, which are the trans-membrane ... 1781was incubated with various effector molecules, and themixture was used as an analyte in SPR analysis. ATP and 2-oxoglutarate are known to be the primary effec-tors involved in PII signaling, ... [3], and some other target promoters[1,4,5]. TnrA is a negative regulator of glnA and gltAB ,encoding the ammonium assimilatory enzymes gluta-mine synthetase (GS) and glutamate synthase, respec-tively...

Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot

... displayed a luciferase activity of about500-fold lower, albeit with a slight increase at 2-HBPconcentrations of 1, 2, 10 and 20 lm (Fig. 5C). As wecan assume that the expression level of CBP–DA-HbpR ... positions. That the rigidity of thebinding region was not inﬂuenced by the change fromfour AT to four or six GC, was shown by mutants 8 and 9 (each with 3· GC and ﬁve mutations). Bothmutants 8 and ... HbpR-regulatable promoters are indicatedschematically with a small triangle and a ‘plus’; the location of thedifferent upstream activation sites (UASs) (C-4 ⁄ C-3, C-2 ⁄ C-1 and D-2 ⁄ D-1) is indicated...

Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR deﬁnes the domain boundaries and identiﬁes an unstructured insertion in the SH2 domain pdf

... 77–78.28 Sasaki A, Inagaki-Ohara K, Yoshida T, Yamanaka A, Sasaki M, Yasukawa H, Koromilas AE & Yoshimura A (2003) The N-terminal truncated isoform of SOCS3translated from an alternative initiation ... D. Scrofani2, Manuel Baca1,† and Raymond S. Norton11 Walter and Eliza Hall Institute, Parkville, Victoria, Australia2 Amrad Corporation Ltd, Richmond, Victoria, Australia3 Department ... is a negative regulator of cytokine signalling that inhibits Januskinase-signal transduction and activator of transcription (JAK-STAT)mediated signal tranduction by binding to phosphorylated...

Báo cáo khoa học: Internalization of the human CRF receptor 1 is independent of classical phosphorylation sites and of b-arrestin 1 recruitment potx

... on the assumption that receptor and ligand are endocytosed together and that the intracellularreceptor–ligand complex can be determined by measuringintracellular radioactive labelled ligand.Following125I-labelled ... 0.4Msucrosefor 20 min and this concentration was maintained duringradioligand incu bation. Data were analysed using Graph-PadPRISMsoftware.ResultsVisualization of agonist-mediated internalizationof ... NP40) and internalized radioactivity wasmeasured. Nonspeciﬁc binding, d etermined in the presenceof 10)7Munlabelled CRF, was subtracted and the radio-activity internalized was expressed as a...

Tài liệu Báo cáo khoa học: Four divergent Arabidopsis ethylene-responsive element-binding factor domains bind to a target DNA motif with a universal CG step core recognition and different ﬂanking bases preference pptx

... were aligned computationally and the appearance of a base at each position in a motif was presented as a percentage fre-quency of all four kinds of base. The base with a frequency higherthan ... cold-, drought- and ABA-regulatedgene expression. Plant Mol Biol 24, 701–713.15 Prabakaran P, An J, Gromiha M, Selvaraj S, UedairaH, Kono H & Sarai A (2001) Thermodynamic databasefor protein-nucleic ... control.No luciferase activity was detected when any of thethree ERF effectors was cotransformed with a reportercarrying DREt1, in which the C8 was replaced byT. Although AtERF1 did not activate transcription...

Tài liệu Báo cáo khoa học: Progress for dengue virus diseases Towards the NS2B–NS3pro inhibition for a therapeutic-based approach ppt

... years as a result ofthe expansion of the Aedes aegypti mosquito to dif-ferent geographic areas, and DHF has spread fromSouth East Asia to the Western Paciﬁc and theAmericas. A substantial ... multifunctional protein with an N-terminal protease domain (NS3pro) (1–180), an RNA triphosphatase, an RNA helicase and an RNA-stimulated NTPase domain in the C-terminal region[26,27]. The protease and ... clinical phases Ior II, and prevention through vaccination has become a major priority onthe agendas of the World Health Organization and of national ministriesof health and military organizations....

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... Characterization of the interaction between the plasmamembrane H+-ATPase of Arabidopsis thaliana and a novelinteractor (PPI1)Corrado Viotti, Laura Luoni, Piero Morandini and Maria Ida De ... & Rasi-CaldognoF (1998) Fusicoccin binding to its plasma membranereceptor and the activation of the plasma membraneH+-ATPase. IV. Fusicoccin induces the associationbetween the plasma ... thek0.5value for the PPI1–H+-ATPase interaction atpH 7.0 was at least as low as at pH 6.4 and not affected by FC-induced 14-3-3 binding, indicatingthat the afﬁnity of the H+-ATPase for...

Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

... HEL92.1.7 (A and B) and HEK293 (C and D) cells. Fireﬂy and renilla luciferase activity was assayed 48 h post-transfection; results are presented as mean ﬁreﬂy relative to renilla luciferase activity,expressed ... Thereafter, ﬁreﬂy and renilla luciferase activity was assayed; results are presented as mean ﬁreﬂy relative to renilla luciferase activity, expressed in arbitrary relative luciferase units (RLU ... (C and D) cells. Fireﬂy and renilla luciferase activity was assayed 48 h post-transfection;results are presented as mean ﬁreﬂy relative to renilla luciferase activity, expressed in arbitrary...

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