Báo cáo khoa học: Characterization of Met95 mutants of a heme-regulated phosphodiesterase from Escherichia coli ppt
Báo cáo khoa học: Critical roles of Leu99 and Leu115 at the heme distal side in auto-oxidation and the redox potential of a hemeregulated phosphodiesterase from Escherichia coli pptx
... DosH as a template
and using the following respective 5¢-sense primers: 5¢-gatga
gtcgggagACCcagctggagaaaaaag-3¢,5¢-gatgagtcgggagTTTcag
ctggagaaaaaag-3¢,5¢-ggacccgttttgcgACCtcgaaagtgagc-3¢,
and ... Hirata S, Matsui T, Sasakura Y, Sugiyama S, Yoshim-
ura T, Sagami I & Shimizu T (2003) Characterization of
Met95 mutants of a heme-regulated phosphodiesterase
from Escherich...
Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt
... (5¢-
GATGTCACGCAGAGTGAGCAGGTAG-3¢)/TRHR-7
(5¢-GAGACCATACAGAAC-C-3¢); second set, A5 2 (5¢-
AGAGTGAGCAGGTAGCGAGAGGAG-3¢)/TRHR-8
(5¢-GGGGGTGTAGAGGTTTCTGGAGAC-3¢); third
set, A5 3 (5¢-CGAGAGGAGCATTAGA-TAGATG
CAG-3¢)/TRHR-9 (5¢-GCCGAAATGTTGATGCCCA
GATAC-3¢) (Fig. 1C). ... (5¢-ATAATGGATAA
CGTAACTTTTGCTG-3¢)/TRHR1-4 antisense (5¢-TC
TGTTAAATGTACCTAAGTAGGCA-3¢)andTRHR2-2
sense (5¢-CAGCAAAATGGAAAAT...
Báo cáo khoa học: Characterization and cDNA cloning of a clofibrate-inducible microsomal epoxide hydrolase in Drosophila melanogaster potx
... post-treatment. The poly (A) -RNA was extracted from the
larvae, and 300 ng mRNA of each sample was loaded on a gel. Actin
mRNA served as an internal marker to equate mRNA quantities.
Fig. 9. Alignment ... DIG Labelling Mix (Roche). To
prepare a DmEH probe, primers (5¢-ATGGCGAAC
ATCTGGCCACGAATC-3¢ and 5¢-TTATGAGAAATT
GGCTTTCTGGAC-3¢) were used, and to prepare Actin
5C probe as an inte...
Báo cáo khoa học: Characterization and structural modeling of a new type of thermostable esterase from Thermotoga maritima ppt
... hydrolases and share structural and functional
characteristics, including a catalytic triad, an a ⁄ b-
hydrolase fold and a cofactor independent activity.
The catalytic triad usually consists of a ... et al.
2838 FEBS Journal 274 (2007) 2832–2842 ª 2007 The Authors Journal compilation ª 2007 FEBS
GTCACCTGGTAGTTACTGCCGCCGAAG-3¢,5¢-CGAC
GATCTCAAT
AACTTGATGATTTCAGG-3¢ and 5¢-CTC
CTGAAA...
Báo cáo khoa học: Characterization and synthetic applications of recombinant AtNIT1 from Arabidopsis thaliana doc
... Characterization and synthetic applications of recombinant AtNIT1
from
Arabidopsis thaliana
Steffen Osswald,
1
Harald Wajant
2
and Franz Effenberger
1
1
Institut fu
È
r Organische Chemie, and
2
Institut ... SDS /PAGE a nd stain ed with Coo massie . Last
lane, molecular masses of standards (kDa); lanes 17±19, active fractions
after HiTrap chromatography. (C) E stimation of native m...
Báo cáo khoa học: Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. docx
... 1792AF
AAAAAGCAGGCTTGCTGTTCTAAGTGTACTAGCTGGA
b
) 1792pA::GUS
) 1263AF
AAAAAGCAGGCTCAAATTAAATCGACGGTTGAG
b
) 1263pA::GUS
) 764AF
AAAAAGCAGGCTCAATGTAGTACCAATTGGGGTACC
b
) 764pA::GUS
) 234AF
AAAAAGCAGGCTGAGAAATCTATGGAATAAATAAAAATTAGGG
b
) ... CB-3¢
ActF GATATGGAAAAGATCTGGCATCAC RT-PCR
(Atactin 2)
ActR TCATACTCGGCCTTGGAGATCC RT-PCR
(Atactin 2)
) 2912AF
AAAAAGCAGGCTATGAATTGCTAGAGTTGGTTAATGC
b
) 29...
Báo cáo khoa học: "Toolkit for Multi-Level Alignment and Information Extraction from Comparable Corpora" pptx
... the ACCURAT
toolkit
1
- a collection of tools that are capable of
analysing comparable corpora and extracting
parallel data which can be used to improve the
performance of statistical and ...
such as daily news articles and large knowledge
bases like Wikipedia, are much more widely
available than parallel translation data.
While methods for the use of parallel corpora in
m...
Báo cáo khoa học: "Towards an Adaptive Communication Aid with Text Input from Ambiguous Keyboards" pptx
... telecommunication
(although fast exchange via SMS or e–mail some-
times develops into a synchronous communication
situation). Alternative and augmentative (AAC)
methods typically deal with communication strate-
gies ... to
the space bar is pressed, a dictionary is consulted
to find all words corresponding to the ambiguous
code.
The advantages of an ambiguous keyboard with
word disambigu...
Báo cáo khoa học: Characterization of Met95 mutants of a heme-regulated phosphodiesterase from Escherichia coli ppt
... the Met95Ala
and Met95Leu mutants. It is possible that an unknown
ligand, such as water/hydroxylate anion (or an amino-acid
main chain), coordinates to the Fe(III) in Met95Ala and
Met95Leu mutants ... 23821–23827.
2. Sato, A. , Sasakura, Y., Sugiyama, S., Sagami, I., Shimizu, T.,
Mizutani, Y. & Kitagawa, T. (2002) Stationary and time-resolved
resonance Raman spectra of His77 and Me...
Báo cáo khoa học: Characterization of rat cathepsin E and mutants with changed active-site residues and lacking propeptides and N-glycosylation, expressed in human embryonic kidney 293T cells ppt
... Fukuoka, Japan
2 Department of Oral Molecular Pharmacology, Graduate School of Biomedical Sciences, Nagasaki University, Japan
Cathepsin E (EC 3.4.23.24) is an intracellular aspartic
proteinase of ... Takahashi T, Taggart
RT, Akamine A, Ezaki M, Nakanishi H, Sakai H &
Yamamoto K (1993) Isolation and characterization of
recombinant human cathepsin E expressed in Chinese
hamster...