... actin would interact differently with HSP25 so we analyzed the effect of HSP25 on the polymerization of intact actin and aggregation of heated actin. Interaction of HSP25 with intact actin In the ... and nonphosphorylated multimers of HSP25 are ineffective in the regulation of actin polymerization. The protein seg- ments of monomeric HSP25 involved in th...
Ngày tải lên: 20/02/2014, 23:20
... in uence the GlnK–TnrA interaction, either alone, in the absence of divalent metals, or in combination with ATP and Mg 2+ or Mn 2+ . To resolve the inhibitory effect of ATP on the GlnK–TnrA interaction ... complex, rapid dissociation was observed, reaching the basal levels of resonance units (GlnK free surface) within seconds. To test the effects of various m...
Ngày tải lên: 06/03/2014, 00:21
Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc
... added lipid concentration, F 0 the fluorescence intensity at the beginning of the titration, F 1 the fluorescence at the end of the titra- tion, K d the dissociation constant, and [L tot ] the total ... subunit of the flavivirus E protein (sequence element containing amino acids 98–110 of the flavivirus E protein) may serve as an internal fusion peptide, as it is direc...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc
... 5. Insertion of stefins into DOPC and DOPG monolayers. (A) Kinetic traces of the insertion into DOPG lipid monolayers at initial pressure of 5 mNÆm )1 . The proteins were injected into the sub- phase ... composed of DOPC or DOPG. The insertion of proteins into the monolayer generated an increase in surface pressure, D p, from the chosen initial pressure, p 0 (Fig. 5A)....
Ngày tải lên: 07/03/2014, 17:20
Báo cáo khoa học: Interaction of Sesbania mosaic virus movement protein with the coat protein – implications for viral spread Soumya Roy Chowdhury and Handanahal Subbarao Savithri docx
... and the ND49 mutant showed 80% loss of interaction with NV. These observations suggest that there is a major interacting domain within the N-terminal 49 amino acids of the SeMV MP. This is in contrast ... demon- strating that a degree of interaction between the pro- teins remains. It is possible that another site of interaction beyond the N-terminal 49 residues may...
Ngày tải lên: 15/03/2014, 00:20
Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx
... the amount of recovered protein owing to the presence of a high molar excess of BSA remaining in the recovery buffer. Therefore, a fixed aliquot of the protein was incubated with 1 ng of the indicated ... com- patible with the binding of eEF1A in the CRC (white arrow), the other of apparent molecular mass of about 100 kDa compatible with binding to nuc...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khoa học: Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering doc
... upon binding being small. These thermodynamics indicate that the binding is dominated by hydrophobic interactions, which is in agreement with inclusion complex formation of c-CD. In addition, ... dissociation of GA from the sweet taste receptor, resulting in recovery of the sweet taste. Although neither the concentration of sweet taste receptors on the tongue nor...
Ngày tải lên: 16/03/2014, 14:20
Báo cáo khoa học: Interaction of an 40 kDa protein from regenerating rat liver with the )148 to )124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver pdf
... presence of increasing concen- trations of methyl green, a majo r groove b inding drug [41] and distamycin A, a minor groove binding drug [42]. Increasing concentrations of distamycin A resulted in ... formed by the factor(s) binding on the minor groove of the )148 to )124 region of c-jun To establish the nature of interaction b etween trans-acting factor(s) and t he...
Ngày tải lên: 16/03/2014, 18:20
Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... full-length v-KIND; DKIND1, deletion of KIND1; DKIND2, deletion of KIND2; DKIND1 + 2, deletion of both KIND1 and KIND2; DRasN, deletion of RasN; DGEF, deletion of RasGEF; KIND1, KIND1 domain; KIND2, KIND2 ... together, these results reveal the specific protein–protein interaction between the v-KIND KIND2 domain and the middle CD region of MAP2. To narrow down the region r...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... deformations of the binding site with con- comitant destabilization of the p53–DNA interaction (or even prevention of target recognition by the pro- tein). DNA binding of the C-terminally truncated p53(1–363) ... The higher the probability of formation of the cisplatin IACs within the p53DBSs due to the occurrence of the GG, AG and ⁄ or GNG motifs, the...
Ngày tải lên: 19/02/2014, 05:20