Báo cáo khoa học: Interaction of DAPI with individual strands of trinucleotide repeats Effects on replication in vitro of the AATÆATT triplet docx

Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc

... actinwould interact differently with HSP25 so we analyzed the effect of HSP25 on the polymerization of intact actin andaggregation of heated actin. Interaction of HSP25 with intact actin In the ... andnonphosphorylated multimers of HSP25 are ineffective in the regulation of actin polymerization. The protein seg-ments of monomeric HSP25 involved in the inhibition of actin polymerization ... Eﬀect of heating on the kinetics of polymerization (A) and salt-induced increase of the light scattering (B) of actin. Actin (15 lM)containing 10% or pyrene-labeled actin in buﬀer G were incubated...

Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx

... in uence the GlnK–TnrA interaction, either alone, in the absence of divalent metals, or in combination with ATP and Mg2+or Mn2+. To resolve the inhibitoryeffect of ATP on the GlnK–TnrA interaction ... complex,rapid dissociation was observed, reaching the basallevels of resonance units (GlnK free surface) withinseconds. To test the effects of various molecules on the interaction of TnrA with GlnK, 40 ... GS in the absence of GlnK. In vitro, the TnrA C-terminus is responsible for interactions with either GS orGlnK, and this region appears also to mediate proteolysis, suggesting thatbinding of...

Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc

... added lipidconcentration, F0 the ﬂuorescence intensity at the beginning of the titration, F1 the ﬂuorescence at the end of the titra-tion, Kd the dissociation constant, and [Ltot] the total ... subunit of the ﬂavivirus E protein(sequence element containing amino acids 98–110 of the ﬂavivirus E protein) may serve as an internalfusion peptide, as it is directly involved in interactions with ... electrostatic interactions play a principal role in the binding of E2(7–26) to negatively charged residues. In contrast, a higher contribution of hydrophobic com-pared with electrostatic interactions...

Báo cáo khoa học: Interaction of human steﬁn B in the preﬁbrillar oligomeric form with membranes Correlation with cellular toxicity doc

... 5. Insertion of steﬁns into DOPC and DOPG monolayers. (A)Kinetic traces of the insertion into DOPG lipid monolayers at initialpressure of 5 mNÆm)1. The proteins were injected into the sub-phase ... composed of DOPC or DOPG. The insertion of proteins into the monolayer generated anincrease in surface pressure, D p, from the chosen initialpressure, p0(Fig. 5A). At p0¼ 5mNÆm)1, insertion of the ... kinetics of insertion than the aggregates at pH 4.8. The kinetics observed for thesetwo cases were quite complex and it is possible that interaction with the monolayer induces cooperativeconformational...

Báo cáo khoa học: Interaction of Sesbania mosaic virus movement protein with the coat protein – implications for viral spread Soumya Roy Chowdhury and Handanahal Subbarao Savithri docx

... and the ND49 mutant showed 80% loss of interaction with NV. These observations suggestthat there is a major interacting domain within the N-terminal 49 amino acids of the SeMV MP. This is in contrast ... demon-strating that a degree of interaction between the pro-teins remains. It is possible that another site of interaction beyond the N-terminal 49 residues maycontribute to these weak interactions. In ... column 4, in Fig. 8A,B). These results show that the interaction of ABFig. 7. Mapping of the SeMV MP domain responsible for interaction of MP with NV. (A) Puriﬁcation of GST–MP deletion mutants....

Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx

... the amount of recovered protein owing to the presence of a high molar excess of BSA remaining in the recovery buffer. Therefore, a ﬁxed aliquot of the proteinwas incubated with 1 ng of the indicated ... com-patible with the binding of eEF1A in the CRC (whitearrow), the other of apparent molecular mass of about100 kDa compatible with binding to nucleolin (blackarrow). The competition experiments in ... nothomogenous, the ratios of the different binding signalswithin each lane instead of their absolute values haveto be considered. It can be seen that GT is presentmainly in the CRC and in a minor complex...

Báo cáo khoa học: Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering doc

... upon binding being small. These thermodynamics indicatethat the binding is dominated by hydrophobic interactions, which is in agreement with inclusion complex formation of c-CD. In addition, ... dissociation of GA from the sweet taste receptor, resulting in recovery of the sweet taste. Although neither the concentration of sweet taste receptors on the tongue nor the bindingafﬁnity of GA to the ... determined, the need for c-CD in the mm range would correlate with the binding afﬁnity between GA and the receptor. The thermodynamic parameters obtained are in the range of those of other interactions...

Báo cáo khoa học: Interaction of an 40 kDa protein from regenerating rat liver with the )148 to )124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver pdf

... presence of increasing concen-trations of methyl green, a majo r groove b inding drug [41]and distamycin A, a minor groove binding drug [42].Increasing concentrations of distamycin A resulted in ... formed by the factor(s) binding on the minor groove of the )148 to )124 region of c-junTo establish the nature of interaction b etween trans-actingfactor(s) and t he )148 to )124 r egion of c-jun,drugsthatspeciﬁcally ... C1.Factor(s) involved in the C2 complex formation pre-exist in the cytosol In order t o establish w hether the synthesis of the rRLjunRP in rRNE-d, responsible for complex C2 for mation (conver-sion of...

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... full-lengthv-KIND; DKIND1, deletion of KIND1;DKIND2, deletion of KIND2; DKIND1 + 2,deletion of both KIND1 and KIND2; DRasN,deletion of RasN; DGEF, deletion of RasGEF; KIND1, KIND1 domain; KIND2,KIND2 ... together, these resultsreveal the speciﬁc protein–protein interaction between the v-KIND KIND2 domain and the middle CDregion of MAP2.To narrow down the region responsible for v-KINDbinding within ... data indicate that the MAP2-binding core site of v-KIND is sufﬁcient for the v-KIND-MAP2 interaction within cells. The MAP2-binding core module of v-KIND isinvolved in targeting to neuronal...

Tài liệu Báo cáo khoa học: DNA modiﬁcation with cisplatin affects sequence-speciﬁc DNA binding of p53 and p73 proteins in a target site-dependent manner pptx

... deformations of the binding site with con-comitant destabilization of the p53–DNA interaction (or even prevention of target recognition by the pro-tein).DNA binding of the C-terminally truncatedp53(1–363) ... The higher the probability of formation of the cisplatinIACs within the p53DBSs due to the occurrence of the GG, AG and ⁄ or GNG motifs, the stronger the inhibition of the p53 sequence-speciﬁc DNA bindingto ... cis-platination, whereas interaction of the protein with the p21b target remained practically unaffectedFig. 4. Effects of DNA modiﬁcation with cisplatin on full-length p53binding to the mdm2,...

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