Báo cáo khoa học: Fragments of pro-peptide activate mature penicillin amidase of Alcaligenes faecalis pdf

... Fragments of pro-peptide activate mature penicillin amidase of Alcaligenes faecalis Volker Kasche, Boris Galunsky and Zoya Ignatova Institute of Biotechnology II, Technical ... excision of a small internal pro-peptide. The function of the pro-region is so far unknown. In vitro addi- tion of chemically synthesized fragments of the pro-peptide to purified matu...

Ngày tải lên: 16/03/2014, 23:20

... microtiter plates in a final volume of 200 lL of complete cell culture medium. After 48 h, the cells were incubated in the absence or presence of 0.5 mm DA. After 24 h of incubation at 37 °C, 5% CO 2 , ... membrane expression of DAT A potential effect of p25a on DAT function was addressed by expressing DAT alone and in the presence of p25a in HEK-293-MSR cells. To validate the sp...

Ngày tải lên: 14/02/2014, 21:20

... of C24. Doc. S1b. sbml format of model structure of C24. Doc. S2a. Model structure of Rsch. Doc. S2b. sbml format of model structure of Rsch. Doc. S3. Documentation of the model structure. This ... rate of net starch synthesis (r CO 2 !Starch )was deter- mined by interpolation of measured starch contents (unit: C 6 ) and calculation of the first derivative of this functio...

Ngày tải lên: 14/02/2014, 22:20

... induces a state of ‘leptin resistance’ in obese animals and humans resulting in development of hyperleptinemia. Similarly, hyperleptinemia is a consequence of the development of insulin resistance in ... endoplasmic reticulum stress) of adipose tissue. These findings support the possibility that the alteration of circulating ANGPTL2 levels could serve as a marker of obesity-induc...

Ngày tải lên: 14/02/2014, 22:20

... chan- nel that divides a pair of acidic lobes, one of which is composed of residues from the SET-I region and the other of residues from the SET-C and post-SET regions. Lysine 4 of histone H3 is inserted ... gene. MLL1 is a member of the evolutionarily conserved SET1 family of histone H3 lysine 4 (H3K4) methyltransferases, which are required for the regula- tion of distinct gr...

Ngày tải lên: 16/02/2014, 14:20

... regulation of transcriptional activity of nuclear factor kappaB [19], the regulation of NAD(P)H oxidase [20], the shedding of human thyrotropin receptor ectodomain [21], the aggregation of Cu ⁄ ... excitation pathlength of 4 mm. Because of these opposing effects, the fluo- rescence of the peptide is quenched, and it contributes a smaller proportion of the total fluorescence o...

Ngày tải lên: 16/02/2014, 14:20

... role of GIF in neurofibrillary changes associated with AD. Potential role of metal-binding/exchange properties of GIF in AD One of the primary pathological hallmarks of AD is the formation of b-amyloid ... regulating synaptic levels of zinc, as the GIF-deficient mice had a reduced content of Zn(II) within a number of brain regions [28]. ROS scavenging ability of GIF The coor...

Ngày tải lên: 16/02/2014, 15:20

... residues can adopt a variety of con- formations, whereas the rest of the structure of cathep- sin B appears to be rigid. A comparison of the interaction constants of the binding of chagasin (K i = 0.93 ... different enzymes is the average distance between the centre of mass of CA atoms of the stefin molecule and the centre of mass of the CA atoms of the reactive site cy...

Ngày tải lên: 18/02/2014, 04:20

... percentage of amino acid coverage in the matched proteins; M pep , the number of unique matched peptides; U pep , the number of unmatched peptides in the MALDI-TOF experiments. The function of new ... spectrum of protein 5, fraction 5 ⁄ fraction 6. Fig. S7. MS spectrum of protein 8, fraction 5 ⁄ fraction 6. Fig. S8. MS spectrum of protein 11, fraction 5 ⁄ fraction 6. Fig. S9. MS...

Ngày tải lên: 18/02/2014, 08:20

... dynamics of extracellular components); EI, enzyme I (protein, component of the PTS); EIIA, enzyme IIA (protein, component of the PTS, ‘output’ of the system as it activates the synthesis of cAMP); EIIBC ... this function, is one of the key players of catabolite repression. Mathematical modelling of signal transduction and gene expres- sion of the enzymes involved in the tran...

Ngày tải lên: 18/02/2014, 13:20