Báo cáo khoa học: Recombinant derivatives of the human high-mobility group protein HMGB2 mediate efficient nonviral gene delivery pptx

... 4221–4236 ª 2005 FEBS Recombinant derivatives of the human high-mobility group protein HMGB2 mediate efficient nonviral gene delivery Arjen Sloots and Winfried S. Wels Chemotherapeutisches Forschungsinstitut, ... we have generated recombinant derivatives of the human high-mobility group (HMG) protein HMGB2 and investigated their potential as novel...

Ngày tải lên: 16/03/2014, 22:20

... likely due to an elongated shape of the protein. The same behavior is observed in the presence of GDPÆAlF x . Because of the unexpected observation of a monomeric protein species with both GDP and ... account). The size of the protein complex is only slightly increased when nucleotide-free or in the presence of GTP. As with hGBP5ta, the complex is increased to...

Ngày tải lên: 15/03/2014, 10:20

... room temperature in the presence of Factor Xa (Novagen). This step removes the His 10 tag to generate the truncated SUMO-2 protein (9–93 amino acids). The protein solution was then purified a second ... residues 45–58 in the former model and 40 , 49 and 55–58 in the latter model of SUMO-2 and the equivalents of ubiquitin. Although the sequences have only 18% identity,...

Ngày tải lên: 16/03/2014, 18:20

... (1994) Identification of a GC element in the promotor of the murine L32 ribosomal protein L32 gene and the 3¢-ter- minal region of the human apolipoprotein A-I gene, capable of binding with murine and human ... GCC-elements of the rpL32 promoter and the 5¢-UTR of the human FMR1 gene. We further identify p56 as the Kru ¨ ppel- like transcription factor Z...

Ngày tải lên: 07/03/2014, 05:20

... National University of Singapore, Singapore The recent discovery of the Nogo family of myelin inhibitors and the Nogo-66 receptor opens up a very promising avenue for the development of therapeutic agents ... Zn 2+ . Furthermore, the d etermination of the Nogo-40 solution structure offers a starting point for further understanding the interaction between NgR and Nogo-4...

Ngày tải lên: 16/03/2014, 16:20

... clarify the contributions of these proteins in the HRR pathway. During the cytoplasmic actin-remodeling process, the ENA ⁄ VASP family proteins bind to both the barbed end and sides of actin ... Uppsala, Sweden) per milligram of the protein. On the other hand, the His6-tagged EVL(222–418) fragment bound to hydroxy- apatite resin. Therefore, the resin containing the...

Ngày tải lên: 23/03/2014, 04:20

... that the N-terminal domain of the DMC1 protein functions as part of the DNA-binding path. Materials and methods Purification of the human DMC1 protein and the DMC1-I37N variant The human DMC1 protein ... that the DMC1-I37N variant was thermally unstable, com- pared with the conventional DMC1 protein (Fig. 1B). We then tested the ATPase activity of the DM...

Ngày tải lên: 23/03/2014, 06:20

... Interestingly, the human eppin gene is located on chromosome 20 in a cluster of 13 other WAP domain containing gene sequences [13,16]. Some of the proteins expressed from these genes also have ... of the bacteria with the proteins, no reduction of the XTT was observed (data not shown). This suggests that the bacteria were now dead and also that the reduction of X...

Ngày tải lên: 23/03/2014, 07:20

... because the localization of Sm proteins at SC35 speckles is more RNA-dependent than the Sm proteins that are diffusely distributed through- out the nucleoplasm. No Sm protein could be detected in theSC35specklesafterRNasetreatment(Fig.3B,eandf), as ... protein. The additional recruitment of FBX4 may stimulate the ubiquitination of the speckle protein. Acknowledgements W...

Ngày tải lên: 23/03/2014, 13:20

... expression of the human PRDX5 gene in situ was not stimulated by the oxidative agent menadione. Silencing of the NRF2 gene in the absence of oxidative stress by specific siRNA did not decrease PRDX5 protein ... 6C). Therefore, knockdown of the PRDX5 gene stimulated transcription of some families of human satellite DNA, which may be caused by direct involvement...

Ngày tải lên: 30/03/2014, 11:20