Báo cáo khoa học: Kinetic mechanism for p38 MAP kinase a A partial rapid-equilibrium random-order ternary-complex mechanism for the phosphorylation of a protein substrate potx
Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity pptx
... that of 4 by the presence of a 2-acetamide group adjacent to the p-nitro- phenyl group. This was the same in the case of a comparison of 1 and 3. Furthermore, the enzyme also catalysed a transglycosylation ... respectively. Assay of endo-b-galactosidase activity by HPLC The standard assay was carried out as follows. A reaction mixture (500 lL) containing an appro...
Ngày tải lên: 31/03/2014, 07:20
Báo cáo khoa học: Kinetic mechanism for p38 MAP kinase a A partial rapid-equilibrium random-order ternary-complex mechanism for the phosphorylation of a protein substrate potx
... mechanism for p38 MAP kinase a Kinetic mechanism for p38 MAP kinase a A partial rapid-equilibrium random-order ternary-complex mechanism for the phosphorylation of a protein substrate Anna E. Szafranska 1 and ... three-tiered MAPK modules, each of which consists of a MAP kinase (MAPK), a MAPK kinase (MAPKK) and a MAPKK kinase...
Ngày tải lên: 16/03/2014, 22:20
Tài liệu Báo cáo khoa học: Kinetic basis for linking the first two enzymes of chlorophyll biosynthesis doc
... quenched-flow analysis reveals that ChlH dramat- ically accelerates the formation and breakdown of an intermediate in the catalytic cycle of ChlM. In light of the profound effect that ChlH has on the methyltransferase ... a higher available concentration of ChlM to bind other mole- cular species in the reaction. ChlH appears to enhance catalysis by accelerating the format...
Ngày tải lên: 20/02/2014, 02:21
Báo cáo khóa học: ICAM-1 expression is highly NF-jB-dependent in A549 cells No role for ERK and p38 MAPK docx
... cells. As noted above, both the p38 and ERK MAPK cascades are variously implicated in the activation of NF- jB. However, as many of these studies rely on transfection analysis of reporter plasmids, ... involve- ment of the p38 mitogen activated protein kinase (MAPK) and the extracellular signal-regulated kinases (ERK) in the activation or potentiation of NF-jB-depe...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Kinetic deuterium isotope effects for 7-alkoxycoumarin O-dealkylation reactions catalyzed by human cytochromes P450 and in liver microsomes Rate-limiting C-H bond breaking in cytochrome P450 1A2 substrate oxidation pdf
... coumarin, the value was high for P450 1A2 (9.5); with 7-OMe coumarin the value for P450 1A2 was 10.6 and for P450 2E1 was 7.7. The value of these measurements lies in their comparison with the values ... microsomes. Results and discussion Intramolecular kinetic isotope effects The intramolecular kinetic isotope effects measure the comparative rates for cleavage of...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khoa học: Kinetic hybrid models composed of mechanistic and simplified enzymatic rate laws – a promising method for speeding up the kinetic modelling of complex metabolic networks pptx
... designates the type of load parameter varied and the range of variation relative to the normal value of the reference state. The last four columns show the percentage of the total variation range of the ... 2008 The Authors Journal compilation ª 2008 FEBS for the construction of the Jacobian matrix used for the analysis of stability. Enzymatic rate la...
Ngày tải lên: 23/03/2014, 06:20
Báo cáo khoa học: "Veterinary decision making in relation to metritis - a qualitative approach to understand the background for variation and bias in veterinary medical records"
... collection of the data in and of itself as the basis for taking relevant action at the farm. They may skip the process of systematic anal- ysis of data and give advice based on their immediate eval- uation ... or her evaluation of the local context. That is, treatment data as an indicator of a certain disease manifestation may only be valid within the herd. When ve...
Ngày tải lên: 25/10/2012, 10:45
Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf
... recombinant enzymes free of albumin and any other AAAs. It was found that there was no fundamental difference in the mechanisms of inhibition and activation for either the AAA or esterase activities ... 7.0 at 25 °C. The effect of each ligand on related pairs of substrates (neutral substrates o-NAC and o-NPA, and positively charged substrates ATMA and ASCh) was compared. To av...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx
... m M Mes (pH 7) and substrate (5¢-G 2 CCCUCUAAAAA-3¢)at50°C [6]. c Substrate- cleavage reaction (exogenous guanosine-mediated) of the substrate (5¢-CUUAAAAA-3¢) using the Anabaena ribozyme (L-8 ... dissociate, the similar order of magnitude suggests that a non-trivial fraction of the substrate will dissociate before the substrate- cleavage reaction occurs. The ‘cata...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khoa học: Cell surface nucleolin on developing muscle is a potential ligand for the axonal receptor protein tyrosine phosphatase-r ppt
... identify the extracellular ligands of the RPTPs in the neuromuscular system. For example, it is known that PTPd and an isoform of LAR can interact homophilically [39] and that LAR can also bind heterophilically ... black dots, protease cleavage sites. (B) SDS ⁄ PAGE separation of FN3d–AP purified from conditioned media using anti-placental alkaline phosphatase (PLAP) agarose. (C)...
Ngày tải lên: 19/02/2014, 05:20