Báo cáo khoa học: cAMP inhibits CSF-1-stimulated tyrosine phosphorylation but augments CSF-1R-mediated macrophage differentiation and ERK activation potx

... tyrosine phosphorylation but augments CSF-1R-mediated macrophage differentiation and ERK activation Nicholas J. Wilson 1, *, Maddalena Cross 3 , Thao Nguyen 3 and John A. Hamilton 1,2,3 1 Arthritis and ... and cAMP in M1/WT cells. Discussion We have provided evidence that cAMP can enhance CSF-1-induced macrophage differentiation in M1/WT A B C FE D Fig. 3. c...

Ngày tải lên: 16/03/2014, 22:20

... catalytic subunit of cAMP dependent protein kinase; CRE, cAMP response element; CREB, cAMP response element-binding protein; db -cAMP, dibutyryl cAMP; IBMX, isobutylmethylxanthine; PKA, cAMP- dependent ... [ 35 S]methionine- labelled mitochondrially encoded subunits of oxidative phosphorylation complexes in rat liver mitochondria. Addition of cAMP or dibutyryl cAMP (db -cAMP) A...

Ngày tải lên: 07/03/2014, 02:20

... Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling. Cell 80, 225–236. 14 Hunter T (1998) The role of tyrosine phosphorylation in cell growth and disease. ... led to the identification of tyrosine phosphorylation sites of mouse and human emerin. Results To identify tyrosine- phosphorylated nuclear-envelope proteins and their phosp...

Ngày tải lên: 07/03/2014, 12:20

... oridonin-treated cells, ERK and phosphorylated ERK (p -ERK) protein levels were determined by western blot analysis. The level of ERK was not obviously changed and the p -ERK level was markedly ... roles of p38 and NF-jB in oridonin-induced apoptosis and autophagy, and found that they both inhibited apop- tosis but promoted autophagy. We demonstrate that p38 contributed to...

Ngày tải lên: 18/02/2014, 13:20

... abstraction, addition and elimination reactions at both the amino acid side chains and backbone amide bonds to produce oxidized, degraded, and cross-linked proteins [2,5,6]. The oxidized cross-linked products and ... fibril formation for WT and V30M TTR and their oxidized forms were monitored by turbidity measurement at 330 nm and 400 nm. These absorbance measurements detect both...

Ngày tải lên: 19/02/2014, 05:20

... a variety of cell types and several mechanisms, such as activation of the Jun N-terminal kinase pathway, involvement of the caspase activated DNAase, the redistribution of p53 and the activation of Bad, ... Bishop J, Peck A, Brown J, Wilson L & Panda D (2004) Antimitotic antifungal compound beno- myl inhibits brain microtubule polymerization and dynamics and cancer cell prol...

Ngày tải lên: 07/03/2014, 05:20

... Bacitracin A and F and bacitracin B and H were purified as pooled fractions of B 1–3 and H 1–3. (C) MALDI-TOF ⁄ TOF MS spectrum of bacitracin F: The most significant fragment ions, their structures and ... much lower, and closer to the IC 50 of the less abundant bacitracin H and F (40 and 20 lm, respectively). We believe that, along with bacitracin H and F, there are other mor...

Ngày tải lên: 14/03/2014, 23:20

... Montan ˜ oLF&Lo ´ pez-Marure R (2005) Dehydroepiandrosterone inhibits the prolifera- tion of HUVEC by enhancing the expression of p53 and p21, restricting the phosphorylation of RB, and is androgen- and estrogen-receptor ... estrogen and androgen, because use of antagonists to androgen and estrogen receptors (flutamide and ICI 182,780, respectively), and letrozol, an inhi...

Ngày tải lên: 16/03/2014, 00:20

... versus mitochondria from 8Br -cAMP treated pRc ⁄ CMVi antisense trans- fected cells. b a 0 4 8 12 16 20 A Control 8Br -cAMP 8Br -cAMP + BPB B Control 8Br -cAMP 8Br -cAMP + BPB AA b a )stinuyrartibra(A 8Br -cAMP + BPB 0 25 Control ... of the phenotype seen in the neuropathology and neurological disorders such as Alzheimer’s, Parkinson’s and Huntington’s dis- eases, and brain injury...

Ngày tải lên: 16/03/2014, 12:20

... 1·. Gels were dried and autoradiography visualized by using GP-Storage Phosphor Screen and storm scanner and software (Amersham Biosciences). Radioactive bands were quantified and annealing percentage ... proteolysis, and several putative phosphorylation sites [1,10,21,22] (Fig. 1). The relationship between CNBP developmental behaviour and phosphorylation has not been analysed...

Ngày tải lên: 16/03/2014, 12:20