Báo cáo khoa học: Signal peptide hydrophobicity is critical for early stages in protein export by Bacillus subtilis ppt

... well as for mapping domains of CPs involved in this process. The recombinant NLPs, with an average length of 50 nm, represent 20–30% of the total purified proteins. The remaining protein is essentially ... PapMV CP indicated that an essential domain for CP multimerization is located on 26 amino acids of the N-terminus [9,10]. In this work, we investigated this region in detail,...

Ngày tải lên: 07/03/2014, 05:20

... of proteins, which in vertebrates, comprises of p65 (RelA), p50/p105, p52/p100, c-Rel and RelB [3]. Within resting cells, NF-jB is retained in the cytoplasm complexed to an inhibitor protein ... binding protein (TBP) to increase transactivation of the DNA bound NF-jB [9], or phos- phorylation of serine 276 of p65 or cAMP response element binding protein (CREB) binding protein (C...

Ngày tải lên: 07/03/2014, 15:20

... vacuolar protein sorting; dopamine responsive gene-1; LYST-interacting protein 5; suppressor of K + uptake growth defect 1 (SKD1); SKD1-binding protein 1 Correspondence A. L. Munn, Institute for Molecular Bioscience, ... vitro binding assay. In addition, the retention of native structure in b domain mutant proteins is supported by the ability of a b domain mutant protein to...

Ngày tải lên: 16/03/2014, 13:20

... 1C). Increased nucleolin/C23 protein levels in Hsp70-transfected cells in response to H 2 O 2 -induced apoptosis We next examined the expression of nucleolin ⁄ C23 protein in these cells in response ... Hsp70 may inhibit apoptosis at multiple points and through a wide range of mechanisms. Nucleolin is a major nucleolar phosphoprotein that belongs to a large family of RNA-binding...

Ngày tải lên: 16/02/2014, 09:20

... Transporter-to-trap conversion: a disulfide bond formation in cellular reti- noic acid binding protein I mutant triggered by retinoic acid binding irreversibly locks the ligand inside the protein. Biochemistry 46, 13382–13390. 13 ... Baptista AM, Gianazza E, Tomaselli S, Molinari H & Ragona L (2008) Conformational and dynamics changes induced by bile acids binding to chicken liver...

Ngày tải lên: 18/02/2014, 06:20

... initiation factor 4E-binding protein (4E-BP1) with the mRNA cap-binding factor eIF4E in cells Keywords caspases; interferon-a; polypeptide chain initiation; protein synthesis; TRAIL Correspondence M. ... together (reviewed in [7,22]), and the enhanced inhibition of protein synthesis by TRAIL observed in IFN-treated MCF-7 and HeLa cells is clearly related to this. However, this...

Ngày tải lên: 19/02/2014, 06:20

... substrate binding drives a conformational change to form a Michaelis complex in which steric compression is intro- duced between the histidine and carboxylate (aspartate in chymotrypsin, glutamate in ... show that for A328F 71% remains, for A328I 32% remains, for wild-type 40% remains, and for A328G 42% remains. In all four cases, the remaining activity at 1 pH unit below th...

Ngày tải lên: 20/02/2014, 23:20

... translation indirectly by inhibition of mRNA synthesis during amino acid starvation [20–23]. Kirromycin is one in a family of antibiotics that inhibits protein synthesis by binding EF-Tu–GTP and preventing the ... are known to be involved in either protein protein interactions [61,62] or protein RNA interactions [63]. The proposed intramolecular interaction may be an integrated...

Ngày tải lên: 21/02/2014, 00:20

... detected by in- gel activity staining (shown only for complex I) are indicated by Roman numerals. The sequenced subunit is shown by an ellip- sis; arrows point to a unique subunit present only in the ... °C. Cultivation and isolation of kinetoplasts P. serpens strain 1G, originally isolated from its insect vec- tor, was cultured in brain heart infusion medium with 10 lgÆmL )1 h...

Ngày tải lên: 07/03/2014, 09:20

... Engineering, Ghent University, Belgium 2 Institute for Plant Biotechnology for Developing Countries (IPBO), Ghent University, Belgium 3 Laboratory of Protein Biochemistry and Protein Engineering, ... extensively studied, very little is known about the cysteine proteinases of Vasconcellea spp. In this investiga- tion, several cDNA sequences coding for cysteine proteinases in Va...

Ngày tải lên: 07/03/2014, 11:20