Báo cáo khoa học: Netropsin interactions in the minor groove of d(GGCCAATTGG) studied by a combination of resolution enhancement and ab initio calculations pot

... interactions FEBS Journal 272 (2005) 3531–3541 ª 2005 FEBS 3533 Netropsin interactions in the minor groove of d(GGCCAATTGG) studied by a combination of resolution enhancement and ab initio calculations Kristof ... (NAE) was investigated by evaluating the interaction energies and hydrogen positions of the end fragment and the bases of bas...

Ngày tải lên: 16/03/2014, 22:20

... proteins contain an N-terminal SAP domain, an SP-RING domain, a SUMO interaction motif (SIM) and a C-terminal domain (CTD) [39]. (B) Pc2 contains an uncharacterized E3 domain, a ubc9 binding domain and a ... likely that ubc9 and the UFD of uba2 interact at the a1 region. (b) The region below the a1 helix consists mainly of the N-terminal exten- sion of a1 and...

Ngày tải lên: 30/03/2014, 04:20

... fat bodies were collected and RNA lev- els were analyzed using qPCR. Transcript abundance values for AaEcRA, AaEcRB, AaUSP -A, AaUSP-B, AaE7 5A (A) , AaHR3, AaHR4, AaE78, AaHR39, AaHR78 (B) and AabFTZ-F 1A, ... biological repli- cates were analyzed, and Fig. 2 depicts the profile matching both replicates. An increase in transcript abundance for AaEcRA, AaEcRB, AaE7 5A, AaE75B, AaE75...

Ngày tải lên: 18/02/2014, 13:20

... m I , m t2 and m f are m-values associated with each of the identifiable states of the folding pathway and relate to the amount of solvent-exposed surface area in each of these states (see Materials and ... general. Having now established this unequivocally, the above findings are of particular importance in our abil- ity to engineer increased protein–protein interac...

Ngày tải lên: 18/02/2014, 13:20

... indicat- ed. The presence of AO, SsaDH and CH 3 -4-aminobutyrate as substrate were required for maximal activity. In the absence of AO there was no NADPH produced and with AO, SsaDH and 4-amino- butyrate ... FEBS Final steps in the catabolism of nicotine Deamination versus demethylation of c-N-methylaminobutyrate Calin-Bogdan Chiribau 1 , Marius Mihasan 1,2 , Petra Gan...

Ngày tải lên: 19/02/2014, 07:20

... contains a large number of gaps (Table 1). In the case of GGA1, there are 9847 gaps remaining. There- fore, applying the segmentation algorithm to each frag- ment will fail to unveil the characteristic ... var- ious intervals of halting parameter values. The Y-axis in the upper panel shows the cumulative percentage of the values at or below each interval. The Y-a...

Ngày tải lên: 19/02/2014, 07:20

... partic- ularly facilitated in the brain, as the brain contains large quantities of oxidizable lipids and metals, and, moreover, has fewer antioxidant mechanisms than other tissues [8]. Free radicals ... GSH). Pilocarpine-induced SE produces several changes in variables related to the generation and elimination of oxygen free radicals in adult rats [18,30]. An increase i...

Ngày tải lên: 19/02/2014, 16:20

... 2-cyclohexen-1-one. The H18 1A and H18 4A enzymes retain activity in reductive and oxidative half-reactions, and thus do not play an essential role in catalysis. Ligand binding and catalysis is not substantially ... bond in close proximity of the N5 atom. A putative flipping motion of the progesterone molecule along an axis parallel to the FMN plane aligns the C...

Ngày tải lên: 20/02/2014, 02:21

... and the arrowheads indicate the position of Ga q and Ga 16 . The molecular mass standards are indicated. The arrow indicates the position of some unspecific bands. Proteasome degradation of G q proteins ... Johansson et al. Proteasome degradation of G q proteins Proteasome involvement in the degradation of the G q family of Ga subunits Bente B. Johansson, Laura...

Ngày tải lên: 20/02/2014, 03:20

... doi:10.1046/j.1432-1033.2003.03658.x (5¢-CCGAGCTCGAGACGTGAAGCGACAATCTCGC AATCT-3¢) containing an XhoI (Promega) site upstream of the start codon and an antisense primer (5¢-CAGCCAA GCTTCTCACTCTTTGATGAAATGCATCT-3¢) con- taining a HindIII ... Takeharu Miyoshi 1 , Harue Kasuga-Aoki 1 , Takashi Isobe 1 , Takeshi Arakawa 2 , Yasunobu Matsumoto 3 and Naotoshi Tsuji 1 1 Laboratory of Par...

Ngày tải lên: 20/02/2014, 11:20