Báo cáo khoa học: Conformational properties of bacterial DnaK and yeast mitochondrial Hsp70 Role of the divergent C-terminal a-helical subdomain pdf

... 2005 FEBS Hsp70 structure and specificity F. Moro et al. Conformational properties of bacterial DnaK and yeast mitochondrial Hsp70 Role of the divergent C-terminal a-helical subdomain Fernando Moro 1 , ... that the interaction of mtHsp70 PBD with the DnaK ATPase domain modifies the accessibility of the substrate binding site. The thermal stabi...

Ngày tải lên: 16/03/2014, 22:20

... demonstrated the association of this enzyme with the cell wall [16,17]. Many of these studies highlighted the physiological implications of PAO-mediated hydrogen peroxide syn- thesis in the apoplast ... ZmPAO1 and ZmPAO2 promoter sequences [4,9], fused to the b-glucuronidase gene (GUS), were transiently expressed in roots, leaves and ears of barley, and in roots and...

Ngày tải lên: 23/03/2014, 10:21

... occur in the segments that connect the b strands to the a helices of the (b/a) 8 barrel fold [ 14]. The catalytic site is located at the bottom of a cleft between domain B and several of the b ® ... baker's yeast showing 31 and 164% of the activity of the oligo-1,6-glucosidase, respectively (Table 1). Furthermore, the activity of the three enzymes...

Ngày tải lên: 23/03/2014, 21:21

... this review, we empha- size the role of DAPK in the regulation of autophagy. Although several aspects of autophagy regulation have been discovered, further examination of the vari- ous regulatory ... 119 and promotes the dissociation of beclin 1 from Bcl-X L and the induction of autophagy, similar to the mechanism by which JNK1 regulates autophagy by phosphoryla-...

Ngày tải lên: 29/03/2014, 08:20

... place without the requirement of full separation of the TM helices. Since then, crystallographic structures of the a IIb b 3 , a v b 3 and a x b 2 ectodomains [11–13] and two NMR-based structures of the ... C473- C503 hinge, and then performing a slight rotation of the bTD module at the main chain of D606 to dock it against the calf-2 domain with its C-terminus...

Ngày tải lên: 18/02/2014, 04:20

... the role of electrical properties of the plasma membrane in the subcellular distribution of K-Ras. In particular, we investigated (a) the role of surface charge on inner and outer leaflet of plasma ... dis- ruption of the electrical properties of the outer and inner leaflets of plasma membrane and ionic gradients through it. Removal of sialic acid fro...

Ngày tải lên: 19/02/2014, 00:20

... selective and not too potent inhibition of cathepsins are all in favor of the active role of cystatin F in processes of antigen presentation. Further work to localize cystatin F in cells other than the ... with other cystatins, cystatin F is a rather slow binding inhibitor of the cathepsins, characterized by k ass values in the range of 10 6 )10 7 m )1 , and high k d...

Ngày tải lên: 19/02/2014, 17:20

... min of reaction time, the yields of the trimer and the pentamer com- pared to the dimer were 34% and 30% for the wild- type, but 66% and 47% for the D392N mutant (Fig. 5B). Oligosaccharide synthesis ... quantities of the elongated oligomers. The maximum yields of (GlcNAc) 5 and (GlcNAc) 6 , synthesized relatively to the hydrolytic product, (GlcNAc) 2 , were 3...

Ngày tải lên: 20/02/2014, 01:20

... major sumoylation site. The conjugation of SUMO-1 to c-Myb raises the question of the role of this modification with respect to the transcriptional activity of c-Myb. Disruption of the SUMO-1 acceptor ... shown in the control lanes 2, 4 and 6 in Fig. 1A but the use of a higher exposure allows the effects of the mutants to be more evident. Again shifted Myb-b...

Ngày tải lên: 21/02/2014, 00:20

... I in the presence of CI. More precisely, the )21 to )48 and )52 to )87 regions of the top strand and )24 to )53 and )58 to )87 regions of the bottom strand were protected by CI (Fig. 3B). The centers ... affinity of CI to the two operators, and the mecha- nism of action of CI. In the present study, we report the purification of /11 CI to near homogeneity...

Ngày tải lên: 07/03/2014, 00:20