Báo cáo khoa học: Conformational properties of bacterial DnaK and yeast mitochondrial Hsp70 Role of the divergent C-terminal a-helical subdomain pdf

... 2005 FEBS Hsp70 structure and speciﬁcity F. Moro et al. Conformational properties of bacterial DnaK and yeast mitochondrial Hsp70 Role of the divergent C-terminal a-helical subdomain Fernando Moro1, ... that the interaction of mtHsp70PBD with the DnaK ATPase domain modiﬁes the accessibility of the substrate binding site. The thermal stability of the peptide complexes of DnaK, mtHsp70 and chimeric ... [42], thermal unfolding of DnaK gave rise tothree endotherms at 43.5, 57.5 and 74 °C. The ﬁrst and second endotherms have been assigned to the unfolding of the N-terminal ATPase domain and the C-terminal...

Báo cáo khoa học: Barley polyamine oxidase isoforms 1 and 2, a peculiar case of gene duplication docx

... demonstrated the association of this enzyme with the cell wall [16,17].Many of these studies highlighted the physiologicalimplications of PAO-mediated hydrogen peroxide syn-thesis in the apoplast ... ZmPAO1 and ZmPAO2 promotersequences [4,9], fused to the b-glucuronidase gene(GUS), were transiently expressed in roots, leaves and ears of barley, and in roots and leaves of maize with the aid of ... for the symplastic localiza-tion of this isoform [10]. On the contrary, the highersimilarity determined between HvPAO1 and ZmPAO,strongly suggests an apoplastic localization of the HvPAO1 isoform....

Báo cáo khoa học: Substrate recognition by three family 13 yeast a-glucosidases Evaluation of deoxygenated and conformationally biased isomaltosides pptx

... occur in the segments that connect the b strands to the a helices of the (b/a)8barrel fold [ 14]. The catalytic site is located at the bottom of a cleft between domain B and several of the b ® ... baker's yeast showing 31 and 164% of the activity of the oligo-1,6-glucosidase, respectively (Table 1).Furthermore, the activity of the three enzymes was reducedby roughly the same extent, ... OH-groups of the reducing-end ring of the two a-glucosidases, probably re¯ecting theirexposure t o bulk solvent. T he stereoch emical course of hydrolysis by these three members of the retaining...

Báo cáo khoa học: Death-associated protein kinase (DAPK) and signal transduction: ﬁne-tuning of autophagy in Caenorhabditis elegans homeostasis doc

... this review, we empha-size the role of DAPK in the regulation of autophagy.Although several aspects of autophagy regulationhave been discovered, further examination of the vari-ous regulatory ... 119 and promotes the dissociation of beclin 1 from Bcl-XL and the induction of autophagy, similar to the mechanismby which JNK1 regulates autophagy by phosphoryla-tion and dissociation of beclin ... 3), mainly in the genetically tractablemodel system, C. elegans.Pro-survival roles of autophagyStarvation response The genetic screens in yeast that led to the identiﬁca-tion of the Atg genes...

Tài liệu Báo cáo khoa học: Solution properties of full-length integrin aIIbb3 reﬁned models suggest environment-dependent induction of alternative bent ⁄extended resting states doc

... placewithout the requirement of full separation of the TMhelices. Since then, crystallographic structures of the aIIbb3, avb3 and axb2ectodomains [11–13] and twoNMR-based structures of the ... C473-C503 hinge, and then performing a slight rotation of the bTD module at the main chain of D606 to dock itagainst the calf-2 domain with its C-terminus in prox-imity of the N-terminus of the b3‘open’ ... including the mod-eled E764-D774 and G840-Q873 loops, the attachment of the ectodomain to the TM helices, and the inter-faces between the calf-2 and bTD modules, are alsoshown in Fig. 3E–G. Note the...

Tài liệu Báo cáo khoa học: Electrical properties of plasma membrane modulate subcellular distribution of K-Ras ppt

... the role of electrical properties of the plasmamembrane in the subcellular distribution of K-Ras. Inparticular, we investigated (a) the role of surfacecharge on inner and outer leaﬂet of plasma ... dis-ruption of the electrical properties of the outer and inner leaﬂets of plasmamembrane and ionic gradients through it. Removal of sialic acid from the outer plasma membrane caused a redistribution of ... alipha-tic, and X is any other amino acid) at the C-terminal end of each Ras isoform is ﬁrst modiﬁed in the cytosolby a farnesyl anchor to the cysteine residue. The AAXsequence is then cleaved...

Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt

... selective and not too potent inhibition of cathepsins are all in favor of the active role of cystatinF in processes of antigen presentation. Further work tolocalize cystatin F in cells other than the ... with othercystatins, cystatin F is a rather slow binding inhibitor of the cathepsins, characterized by kassvalues in the range of 106)107m)1, and high kdissvalues in the range of 10)3to ... the true exopeptidases cathepsins C(EC 3.4.14.1) and X (EC 3.4.22 ) were not inhibitedat all, even at the highest concentration of the inhib-itor (200 nm for cathepsin C and 600 nm for cathep-sin...

Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx

... min of reaction time, the yields of the trimer and the pentamer com-pared to the dimer were 34% and 30% for the wild-type, but 66% and 47% for the D392N mutant(Fig. 5B).Oligosaccharide synthesis ... quantities of the elongated oligomers. The maximum yields of (GlcNAc)5 and (GlcNAc)6, synthesized relatively to the hydrolytic product, (GlcNAc)2, were 3% and 9%for the wild-type enzyme and 11% and ... formation, the release of dimers, trimers and tetramers from the hexamer wasconsidered to result from direct action of the enzymes.On the other hand, the pentamer appeared to beformed by the condensation...

Tài liệu Báo cáo khoa học: Transactivation properties of c-Myb are critically dependent on two SUMO-1 acceptor sites that are conjugated in a PIASy enhanced manner pptx

... majorsumoylation site. The conjugation of SUMO-1 to c-Mybraises the question of the role of this modiﬁcation withrespect to the transcriptional activity of c-Myb.Disruption of the SUMO-1 acceptor ... shown in the control lanes2, 4 and 6 in Fig. 1A but the use of a higher exposure allows the effects of the mutants to be more evident. Again shiftedMyb-bands were observed in addition to the main ... K527-conjugationenhances the efﬁciency of sumoylation at the other site.A third line of independent evidence for sumoylation of c–Myb is the interaction between c-Myb and Ubc9, the latter acting as...

Báo cáo khoa học: Physicochemical properties and distinct DNA binding capacity of the repressor of temperate Staphylococcus aureus phage /11 doc

... I in the presence of CI. Moreprecisely, the )21 to )48 and )52 to )87 regions of the top strand and )24 to )53 and )58 to )87 regions of the bottom strand were protected by CI (Fig. 3B). The centers ... afﬁnity of CI to the two operators, and the mecha-nism of action of CI. In the present study, we report the puriﬁcation of /11 CI to near homogeneity and, for the ﬁrst time, present evidence for the ... The N-terminal end sequencing of one of the chymotryp-sin-digested fragments revealed that the junction regionbetween the C-terminal end of the histidine tag and the N-terminal end of the native /11 repressor...

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