Báo cáo khoa học: The study of G-protein coupled receptor oligomerization with computational modeling and bioinformatics doc

... of the combined amide I and II bands, with separate analyses of each band afterwards; and separate normalization of the amide I and II bands. All these normalizations were tested with and without ... . Quality of the fit The match between the original a nd re constructed spectra can be evaluated by taking the difference between the two. The sum of the ab...

Ngày tải lên: 16/03/2014, 18:20

... 5B). These findings, coupled with the strong enrichment of the c-isoform of actin recovered in the BAP31 immunopre- cipitate, indicate that the association between BAP31 and c-actin was the result ... stimulate these interactions [8]. An important aspect of this study was the use of tandem MS to identify the protein bands copurified with the immunoprecipitation...

Ngày tải lên: 20/02/2014, 23:20

... addition, strengthen the hypothesis of the involvement of Ki-1 ⁄ 57 in pre-mRNA processing events. The GEMS are regions enriched with the survival of motor neurons (SMN) protein complexes and are con- sidered ... bromide, the band intensities were calculated using the software image j (http://rsb.info.nih.gov/ij/index.html; National Institute of Mental Health, Bethesda...

Ngày tải lên: 16/03/2014, 02:20

... backbone amides of the segments comprising residues 50–96 of NS2B and 17–187 of NS3pro, with the exception of prolines and a few resi- dues with very broad amide peaks. The resonances of the peptide ... Intermolecular NOEs with the aromatic ring protons of 3 are marked with their assignments. Several of the NOEs are also observed with the methyl group...

Ngày tải lên: 07/03/2014, 02:20

... nmol or 0.66 nmol of rMIH-B at the arthropodial membrane of the periopod and returned to the culture tanks. At 24, 48 and 72 h after injection, the hepatopan- creas and ovary of the shrimp were ... females. The percentage indicates the GSI of the females. M (B) indicates the expression pattern of MIH-B in the same tissues in males (N ¼ 5). The lower panel...

Ngày tải lên: 16/03/2014, 05:20

... esti- mation of the bandwidth and the intensity of the bands making up the amide I or I¢ region. Thereafter, curve fitting was performed and the heights, widths and positions of each band were optimized ... this study we used IR spectroscopy to study the secondary structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and als...

Ngày tải lên: 16/03/2014, 13:20

... complex]) with the presence of a minority of active complexes at the beginning of the reaction (multiple turnovers of the most efficient com- plexes are then limited by the high stability of the IN– DNA ... product. The stability of IN during the time-course of the reaction and the influence of protein aggregation are discussed. Results General features...

Ngày tải lên: 16/03/2014, 13:20

... whether the tyrosine phosphorylation status of tau also affects the binding of tau to Fyn-SH3, CHO cells were cotransfected with Fyn together with either wild-type or the YF mutant forms of tau. ... mobility of tau, with the appearance of an  68-kDa tau species in wild-type and all of the mutant forms of tau except for YallF and Y394F tau (Fig. 3). In contra...

Ngày tải lên: 14/02/2014, 14:20

... in the present study might reflect the impor- tance of the nuclear origin of the target mRNA for the functional outcome of ASF ⁄ SF2 recruitment to mRNA 3¢ UTR. In conclusion, the present study ... On the other hand, other studies indicated that ASF ⁄ SF2 activates the translation of mRNAs bearing ASF ⁄ SF2 binding sites within the coding region in intact cells [1...

Ngày tải lên: 16/02/2014, 15:20

... above. Sequence and molecular phylogenetic analyses Partial sequences were assembled with genedoc 2.7 [41]. The tools provided with the ExPASy Molecular Biology Server of the Swiss Institute of Bioinformatics ... hemocyanin in the stonefly Perla marginata (Plecoptera) and in the firebrat Thermobia domestica (Zygentoma). We used RT-PCR and RACE experiments to study t...

Ngày tải lên: 18/02/2014, 08:20