Báo cáo khoa học: The study of G-protein coupled receptor oligomerization with computational modeling and bioinformatics doc
Báo cáo khoa học: The optimization of protein secondary structure determination with infrared and circular dichroism spectra docx
... of the combined amide I and II bands, with separate analyses of each band afterwards; and separatenormalization of the amide I and II bands. All thesenormalizations were tested with and without ... .Quality of the fit The match between the original a nd re constructed spectracan be evaluated by taking the difference between the two. The sum of the absolute values of the differences between the ... a-helix) and the spectral r esidual from reconstructing unknowns with the factors from a PLS-1 model. The residual is characterized by the sum of the absolute values of the d ifference between the...
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Tài liệu Báo cáo khoa học: The resident endoplasmic reticulum protein, BAP31, associates with c-actin and myosin B heavy chain Analysis by capillary liquid chromatography microelectrospray tandem MS ppt
... 5B).These findings, coupled with the strong enrichment of the c-isoform of actin recovered in the BAP31 immunopre-cipitate, indicate that the association between BAP31 and c-actin was the result ... stimulatethese interactions [8].An important aspect of this study was the use of tandemMS to identify the protein bands copurified with the immunoprecipitation of BAP31. Identification of proteinsseparated ... activation of caspases, as assayed by the processing of procaspases 8 and 3, by the measurement of total DEVDase activity, and by the cleavage of the caspase targets poly(ADP-ribosyl)polymerase and...
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Báo cáo khoa học: Functional association of human Ki-1⁄57 with pre-mRNA splicing events docx
... addition, strengthen the hypothesis of the involvement of Ki-1 ⁄ 57 in pre-mRNA processingevents. The GEMS are regions enriched with the survival of motor neurons (SMN) protein complexes and are con-sidered ... bromide, the band intensities were calculated using the softwareimage j (http://rsb.info.nih.gov/ij/index.html; NationalInstitute of Mental Health, Bethesda, MD, USA). The intensities of all isoforms ... complexes in vivo. The pan-antibody against hnRNPQ recognizes the isoforms hnRNPQ1, hnRNPQ2, and hnRNPQ3, and the antibody against SFRS1 ⁄ 9 recognizes the splicingfactors SFRS9 and SFRS1. The latter...
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Báo cáo khoa học: NMR study of complexes between low molecular mass inhibitors and the West Nile virus NS2B–NS3 protease ppt
... backbone amides of the segmentscomprising residues 50–96 of NS2B and 17–187 of NS3pro, with the exception of prolines and a few resi-dues with very broad amide peaks. The resonances of the peptide ... Intermolecular NOEs with the aromatic ring protons of 3 are marked with their assignments. Several of the NOEs arealso observed with the methyl groups of 3at 2.3 p.p.m.NMR analysis of the West Nile ... of the complex with 1 were assignedusing NS2B–NS3pro, whereas the 3D NMR experi-ments of the complexes with 2 and 3 employed the WNV NS2B–NS3pro(K96A) mutant. The resonanceassignments of the...
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Báo cáo khoa học: The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis ppt
... nmol or 0.66 nmol of rMIH-B at the arthropodialmembrane of the periopod and returned to the culturetanks. At 24, 48 and 72 h after injection, the hepatopan-creas and ovary of the shrimp were ... females. The percentage indicates the GSI of the females. M (B) indicates the expression pattern of MIH-B in the same tissues in males (N ¼ 5). The lower panel is the northernblot analysis of MeMIH-B ... the tiger prawn Penaeus monodon. As there is a high degree of sequence similarity and structural conservation of these genes and gene products, confirmation of the trueidentity and function of...
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Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot
... esti-mation of the bandwidth and the intensity of the bandsmaking up the amide I or I¢ region. Thereafter, curve fittingwas performed and the heights, widths and positions of each band were optimized ... this study we used IR spectroscopy to study the secondary structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also to study the effect of thermal unfolding in the ... H–D exchange. To study the IR amidebands of the protein in the presence of MgATP, the proteinwas prepared in D2O buffer and mixed with the protein toa final concentration of 1 mm.IR spectra...
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Báo cáo khoa học: Kinetic study of the HIV)1 DNA 3¢-end processing Single-turnover property of integrase docx
... complex]) with the presence of aminority of active complexes at the beginning of the reaction (multiple turnovers of the most efficient com-plexes are then limited by the high stability of the IN–DNA ... product. The stability of IN during the time-course of the reaction and the influence of protein aggregation are discussed.ResultsGeneral features of the 3¢-end processing kinetics The kinetics of ... specificity of catalysis is greater with Mg2+ and the choice by the enzyme of the nucleophilefor the 3¢-processing reaction strongly depends on the nature of the cationic cofactor [8–11]. Moreover, sev-eral...
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Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt
... whether the tyrosine phosphorylation status of tau also affects the binding of tau to Fyn-SH3, CHO cells werecotransfected with Fyn together with either wild-typeor the YF mutant forms of tau. ... mobility of tau, with the appearance of an 68-kDa tau species in wild-type and all of the mutant forms of tau except for YallF and Y394F tau (Fig. 3). In contrast to the results obtained with Fyn-SH2, ... disruption of the association of these proteins could represent a poten-tial therapeutic strategy for the treatment of AD. Here,we used GST fusion proteins of Fyn-SH2 and Fyn-SH3 to further investigate...
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Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
... in the present study might reflect the impor-tance of the nuclear origin of the target mRNA for the functional outcome of ASF ⁄ SF2 recruitment tomRNA 3¢ UTR.In conclusion, the present study ... On the other hand, other studies indicated that ASF ⁄ SF2activates the translation of mRNAs bearing ASF ⁄ SF2binding sites within the coding region in intact cells[16,54]. The capacity of ... in the control of gene expression. Aseach step of the RNA metabolism is tightly regulated, the regulation of mRNA export, stability and transla-tion rate is essential for the control of the...
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Tài liệu Báo cáo khoa học: The occurrence of hemocyanin in Hexapoda docx
... above.Sequence and molecular phylogenetic analysesPartial sequences were assembled with genedoc 2.7 [41]. The tools provided with the ExPASy Molecular BiologyServer of the Swiss Institute of Bioinformatics ... hemocyanin in the stonefly Perla marginata(Plecoptera) and in the firebrat Thermobia domestica (Zygentoma). We usedRT-PCR and RACE experiments to study the presence of hemocyanin in abroad range of ametabolous ... possible ‘intermediate’. The evolution of hemocyanin subunits follows the widely accepted phylogeny of the Hexapoda and provides strong evidencefor the monophyly of the Polyneoptera (Plecoptera,...
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