... (approx. 4–8 copies facB) [46] A6 37 yA2, pabaA1, pdhA1 FGSC, Kansas City, KS, USA A6 34 yA2, pabaA1; pdhB4 FGSC, Kansas City, KS, USA A6 27 yA2, pabaA1; pdhC1 FGSC, Kansas City, KS, USA A2 6 biA1; ... MDH a nd citrate synthase and the r esulting absorbanceat 340 nm was measured (A 1). MDH was added and the absorbance after r eaching t he equilibrium was taken as A 2.Citrate synthase was added ... even in the mutant strain.Determination of the intracellular volume In order to obtain the intracellular concentration ofaccumulated acyl-CoA esters, it was necessary to know the internal volume...