Báo cáo khoa học: Affinity of S100A1 protein for calcium increases dramatically upon glutathionylation docx

... Affinity of S100A1 protein for calcium increases dramatically upon glutathionylation Graz_ yna Goch, Sergiusz Vdovenko, Hanna Kozłowska and Andrzej Bierzyn ˜ ski Institute of Biochemistry ... probe of protein- surface hydrophobicity. S -Glutathionylation of the single cysteine residue (85) of the a subunits leads to a 10-fold increase in the affinity of the pr...

Ngày tải lên: 16/03/2014, 19:20

... gain -of- function mutants of PCSK9 reduced binding of [ 125 I]TC-PCSK9. The Scatchard plot of the binding-inhibition curve was curvilinear, indicative of high -affinity and low -affinity sites for ... higher -affinity and lower -affinity binding sites are listed in Table 1. The high -affinity and the low -affinity sites accounted for 25% and 75% of the total binding, respective...

Ngày tải lên: 14/02/2014, 14:20

... disease pathogenesis Familial forms of AD, with an autosomal dominant mode of inheritance, account for < 2% of all AD cases. Onset is most often before 65 years of age, and the penetrance is nearly ... cerebral microvascular accumu- lation of amyloid beta -protein in transgenic mice expressing low levels of a vasculotropic Dutch ⁄ Iowa mutant form of amyloid beta -protein...

Ngày tải lên: 16/02/2014, 09:20

... silver staining. Co-pull-down experiment For further study of the protein protein interaction, we performed a co-pull-down experiment to analyze the num- ber of binding sites of VVA1 and VVA2. VVA1 (VVA2 beads) ... has two binding sites for interaction with two molecules of VVA2, and that large amounts of free VVA2 can use VVA1 as a basis for the formation of VVA2 oligomer...

Ngày tải lên: 19/02/2014, 06:20

... Proceedings of the 12th Conference of the European Chapter of the ACL, pages 157–165, Athens, Greece, 30 March – 3 April 2009. c 2009 Association for Computational Linguistics Web augmentation of language ... size of the web mixture LM is limited to the size of the baseline in-domain LM. 1 Introduction An automatic speech recognition (ASR) system consists of acoustic models...

Ngày tải lên: 22/02/2014, 02:20

... SRSRpT 212 PpS 214 LPpT 217 PPTR peptide of Tau. The cis conformation for the Pro219 is below the limit of detection. Upon addition of a catalytic amount of Pin1, enhanced cis ⁄ trans isomerization of the pThr212-Pro213 ... involve- ment of two major classes of isomerases, the cyclophilins (Cyps) and the FK506-binding proteins (FKBPs), in the protein- folding process, where...

Ngày tải lên: 07/03/2014, 05:20

... December 2006) doi:10.1111/j.1742-4658.2007.05653.x Multisite protein phosphorylation is a common regulatory mechanism in cell signaling, and dramatically increases the possibilities for protein protein interactions, conformational regulation, ... special cases. The order of phos- phate processing and the characteristics of protein protein interactions turn out to be of ove...

Ngày tải lên: 07/03/2014, 10:20

... compound. Quantitative analysis of components was performed by integration of the multiple charged ions of the single species. For protein analysis, the separation was attained with a linear gradient of 20–40% solvent ... the monomeric form and 45% in the trimeric form, and b-structure is absent from both forms. The CD spectra of the 1–70 and 70–90 regions of the protein are...

Ngày tải lên: 07/03/2014, 14:20

... the efficiency of the PCR (Fig. 1B). We performed West- ern blot analysis of total proteins from seedlings of wild-type, atrpa70b, and the AtRPA70b RNAi mutant, and confirmed that very little AtRPA70b protein ... three independent transformants were isolated for each plasmid pair. Liquid cultures of each transformant were prepared and tested for b-galactosidase activity. Shown are...

Ngày tải lên: 07/03/2014, 21:20

... Conference of the Associa- tion for Computational Linguistics. John DeNero and Dan Klein. 2008. The complexity of phrase alignment problems. In Proceedings of the Annual Conference of the Association for ... Cherry and Dekang Lin. 2006. Soft syntactic constraints for word alignment through discrimina- tive training. In Proceedings of the Annual Confer- ence of the Associatio...

Ngày tải lên: 07/03/2014, 22:20