Báo cáo khoa học: Biochemical and spectroscopic characterization of the bacterial phytochrome of Pseudomonas aeruginosa pdf

... therefore the site and kind of attachment of the bilin chromophore in the bacterial phytochromes is controversial [1,21,22]. To investigatewhether the chromophore is attached via a thioetherlinkage ... that the lack of the conserved cysteine residue in the BLD correlates with the use of biliverdin as the chromophore and the bind-ing to a conserved cysteine residue in the P2 domain[25]. Nevertheless, ... Here we describe the biochemi-cal and spectroscopic characterization of recombinant bacterial phyto-chrome from the opportunistic pathogen Pseudomonas aeruginosa (PaBphP). The recombinant protein...

Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf

... products of the hazelnutLOX were compared with the reaction products of soybeanLOX type I and authentic standards of 9- and 13-HODE.As shown in Fig. 4, 9-HODE is the main product of the hazelnut ... position 4235 of the genomic clone as the translation start. The characterization of other clonesrevealed the presence of another cDNA (CaLOX2) sharinghigh identity, 91.9%, with the CaLOX1. The comparison of ... aim to betterclarify the occurrence of LOXs and their inﬂuence onhazelnut seed quality, we carried out the biochemical and molecular characterization of the main LOX isoformsexpressed during...

Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

... to the culture medium [1]. In this study, we havepuriﬁed and characterized one of the laccase isoforms,cloned and sequenced the cDNA encoding the enzymeprotein, and examined the effect of ... only one band wasampliﬁed from each of the different samples and Southern blot analysis using the probe prepared with the lac1 primers also revealed a single band. Further-more, all of these generated ... positively affected the activity and stability of the enzyme [40]. The effect of copper onenzyme stability may be related to the inhibitory effect of the metal on the activity of an extracellular...

Báo cáo khoa học: Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus pptx

... torescue the possible damage of the other two disulﬁdebonds. The presence of a low percentage of the proteinwith Cys254 and Cys256 in the reduced form furthersupports this hypothesis.It ... role in the stability of the enzyme againstthermal inactivation. The characterization of the thermal properties of the C254S ⁄ C256S mutant suggests that the CXC motif in the C-terminalregion ... important for the enzymestability. Finally, the characterization of the thermalproperties of the C254S ⁄ C256S mutant allowed us topropose that the CXC motif in the C-terminal region of PfPNP may...

Báo cáo khoa học: Biochemical and molecular characterization of puriﬁed chicken pancreatic phospholipase A2 docx

... features of the sPLA2s are well conserved: the central a-heliceswith the catalytic His–Asp dyad, and the hydrogen-bonding network connecting the interfacial bindingsite, the catalytic site and the ... of the hydrolysis products [12,19]. In order to investigate the effect of bile salts on ChPLA2activity, the rate of hydrolysis of PC by ChPLA2in the presence of vari-ous concentrations of ... mass of 14 kDa and aspeciﬁc activity of 400 UÆmg)1in the presence of 1 mm sodium taur-odeoxycholate and 4 mm CaCl2with phosphatidylcholine as substrate. The N-terminal sequence of the ﬁrst...

Báo cáo khoa học: Structural and thermodynamic insights into the binding mode of ﬁve novel inhibitors of lumazine synthase from Mycobacterium tuberculosis pptx

... nitrogen and main and side-chain oxygen atoms of Ala59 and Glu61 of one subunit and with the main chain nitro-gen of Asn114¢ of the other subunit. The contacts of the ribityl chain to His89 and Lys138¢ ... series of inhibitors thatmimic the substrate, the intermediates and the product of the reaction [16–22] catalysed by LS. The ﬁrstdetailed description of the active site of LS was providedby the ... kcalÆmol)1for MbtLS ⁄TS50. The analysis of the thermodynamic parameters of the different inhibitors clearly showed an increase of the afﬁnity (decreasing of DG) of the compounds bear-ing the alkyl phosphate...

Báo cáo khoa học: Temperature and salts effects on the peptidase activities of the recombinant metallooligopeptidases neurolysin and thimet oligopeptidase pdf

... verify the inﬂuence of the nature of the amino acid at this position ondetermination of the cleavage sites and on the amount of their hydrolysis. This series of peptides was chosen because the ... bradykinin [5] and neurotensin [6]. An outstanding feature of the hydrolyticactivities of neurolysin and TOP on these substrates is the variability of the cleavage sites, which is a consequence of modiﬁcations ... of hydrolysis and the variations of the cleavage sites wereevaluated under diﬀerent conditions of temperature and salt concentration. The relative amount of cleavage variedwith the nature of the substitution...

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... A3(2).Cloning and expression of the putative hydgenes The deduced open reading frames of putative hydgenes were PCR-ampliﬁed from the genomic DNA of the corresponding organisms. The PCR fragments of the ... performed usingT-COFFEE [44].Docking simulation of substrate on the active site of HYDJs The substrate binding pockets of HYDJs and the mode of interaction between substrate and enzyme were ... for the catalytic activity, even though there may be otherrequirements for residues at other positions, such asside-chain size or polarity. Better understanding of the roles of each of these...

Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

... [57].Isolation and cloning of the SynMGST and E.coliMGST The coding sequence for the SynMGST, corresponding to the complementary strand of the nucleotide sequence from89 254 to 89 667 in the Synechocystis ... MGST1,PGES and Insect in the upper part of the tree and the remainingfamilies ⁄ groups in the lower part. In the lower part, MGST2, FLAP and LTC4 synthase have a close relationship, as judged by the short ... of the predominant band displaying the correct molecular mass, puriﬁed from the gel,yielded the expected sequence. The partially puriﬁedprotein constitutes a major part of the preparation and therefore...

Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

... 29,24 and 12 h at temperatures of 4, 13 and 26.5 °C,respectively.Inhibition of AK by the speciﬁc inhibitor Ap5A and itseffect on growth of L. donovanipromastigotesin vitro The effect of the ... prediction of theoretical three-dimensional structure of the enzyme. The three diﬀerent views wereobtained by simple rotation of the same model. The ﬂexible LIDdomain appears at top of the three ... open and closed conformation to the proteinduring binding of the substrate is shown in the top-mostportion of the protein. ATP may bind to the glycine loopon the left ﬂap portion (Fig. 8B) and...

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