Báo cáo khoa học: Biochemical and spectroscopic characterization of the bacterial phytochrome of Pseudomonas aeruginosa pdf

... therefore the site and kind of attachment of the bilin chromophore in the bacterial phytochromes is controversial [1,21,22]. To investigate whether the chromophore is attached via a thioether linkage ... that the lack of the conserved cysteine residue in the BLD correlates with the use of biliverdin as the chromophore and the bind- ing to a conserved cysteine...

Ngày tải lên: 16/03/2014, 18:20

... products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE. As shown in Fig. 4, 9-HODE is the main product of the hazelnut ... position 4235 of the genomic clone as the translation start. The characterization of other clones revealed the presence of another cDNA (CaLOX2) sharing high...

Ngày tải lên: 21/02/2014, 00:20

... to the culture medium [1]. In this study, we have purified and characterized one of the laccase isoforms, cloned and sequenced the cDNA encoding the enzyme protein, and examined the effect of ... only one band was amplified from each of the different samples and Southern blot analysis using the probe prepared with the lac1 primers also revealed a single band. Furth...

Ngày tải lên: 07/03/2014, 15:20

... to rescue the possible damage of the other two disulfide bonds. The presence of a low percentage of the protein with Cys254 and Cys256 in the reduced form further supports this hypothesis. It ... role in the stability of the enzyme against thermal inactivation. The characterization of the thermal properties of the C254S ⁄ C256S mutant suggests that the CXC...

Ngày tải lên: 23/03/2014, 09:21

... features of the sPLA 2 s are well conserved: the central a-helices with the catalytic His–Asp dyad, and the hydrogen- bonding network connecting the interfacial binding site, the catalytic site and the ... of the hydrolysis products [12,19]. In order to investigate the effect of bile salts on ChPLA 2 activity, the rate of hydrolysis of PC by ChPLA 2 in the...

Ngày tải lên: 30/03/2014, 01:20

... nitrogen and main and side-chain oxygen atoms of Ala59 and Glu61 of one subunit and with the main chain nitro- gen of Asn114¢ of the other subunit. The contacts of the ribityl chain to His89 and Lys138¢ ... series of inhibitors that mimic the substrate, the intermediates and the product of the reaction [16–22] catalysed by LS. The first detailed desc...

Ngày tải lên: 07/03/2014, 11:20

... verify the influence of the nature of the amino acid at this position on determination of the cleavage sites and on the amount of their hydrolysis. This series of peptides was chosen because the ... bradykinin [5] and neurotensin [6]. An outstanding feature of the hydrolytic activities of neurolysin and TOP on these substrates is the variability of the c...

Ngày tải lên: 23/03/2014, 21:21

... A3(2). Cloning and expression of the putative hyd genes The deduced open reading frames of putative hyd genes were PCR-amplified from the genomic DNA of the corresponding organisms. The PCR fragments of the ... performed using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode of...

Ngày tải lên: 18/02/2014, 08:20

... [57]. Isolation and cloning of the SynMGST and E.coliMGST The coding sequence for the SynMGST, corresponding to the complementary strand of the nucleotide sequence from 89 254 to 89 667 in the Synechocystis ... MGST1, PGES and Insect in the upper part of the tree and the remaining families ⁄ groups in the lower part. In the lower part, MGST2, FLAP and LTC...

Ngày tải lên: 19/02/2014, 17:20

... 29, 24 and 12 h at temperatures of 4, 13 and 26.5 °C, respectively. Inhibition of AK by the specific inhibitor Ap5A and its effect on growth of L. donovani promastigotes in vitro The effect of the ... prediction of theoretical three- dimensional structure of the enzyme. The three different views were obtained by simple rotation of the same model. The flexible LI...

Ngày tải lên: 21/02/2014, 00:20