Báo cáo khoa học: Plasmodium falciparum hypoxanthine guanine phosphoribosyltransferase Stability studies on the product-activated enzyme potx

... time-points, on the linear phase of the reaction. Any contribution to the absorbance from the ligand carried over (< 0.48 lm) into the assay together with the activated enzyme would not affect the ... presented. The presence of ligands at these concentrations in the assay did not increase the reac- tion rates of the unactivated enzyme. Surprisingly, acti- vation...

Ngày tải lên: 16/03/2014, 18:20

... mentions have the same spelling; 0 otherwise left_subsm 1 if one mention is a left substring of the other; 0 otherwise right_subsm 1 if one mention is a right substring of the other; 0 otherwise acronym ... entity level, the entity-mention model is able to check the gender consistency when considering the active mention “she”. 3.3 Discussion There is an in-focus entity in the co...

Ngày tải lên: 31/03/2014, 03:20

... site predictions, made using methods based on neural networks, were marked on the alignment. The x-axis shows the position of the alignment, and the y-axis marks the predicted potentials. The horizontal ... enriched in the 19-kDa C-terminal fragments of MSP-1 and in the erythrocytic, GPI-linked CD59. The Triton X-100 soluble fractions of the gradient contained the...

Ngày tải lên: 08/03/2014, 08:20

... present. The result obtained with dithionite confirms this conclusion; indeed, even through the reduction is total, the oxidation is only partial, with the fraction of oxidation corresponding to ... of one electron to the hemes, then to the [Fe-S] cluster, to be finally accumulated at the level of the molybdenum cofactor to be able to undertake the catalytic reaction. The lo...

Ngày tải lên: 07/03/2014, 15:20

... the vectors of concepts that occur in a context win- dow. If many of the concepts in a window have a strong component for one of the topics, then the sum of the vectors, the context vector, will ... between the neighbours of the two words whose vectors are compared, the higher the score. Contexts are represented as context vectors in Concept Space. A context vector is the...

Ngày tải lên: 08/03/2014, 04:22

... protein atoms. The former propionate displays the conserved conformation in the structures of the Phe35fiTyr mutant and of the wild-type protein as well as other mutants. However, the conformation of the ... examining the subtle local conformation changes around the heme group under natural conditions. Therefore, the heme dissociation kinetics at different urea concentratio...

Ngày tải lên: 08/03/2014, 10:20

... identical conditions, all the immuno- reactivity remained on the top of the gradient. Fig. 2. Characterization of the heparin enhanced aggregation of rPrP. (A) Comparison of the aggregation of rPrP ... [2,3]. These mutations are either insertional or point mutations. The insertion mutations occur solely in the octapeptide-repeat region; wild-type human PrP has five octapeptide...

Ngày tải lên: 16/03/2014, 04:20

... unknown whether the dual function of PUF60 on transcription and spli- cing is coupled as in the case of the CAPER proteins or whether PUF60 affects independently the transcrip- tion and splicing ... are located in the same positions as in the human CAPERa gene and the introns are found to be smaller in the CAPERb gene. Given the similarities between the evolutionary hist...

Ngày tải lên: 23/03/2014, 10:20

... extended expression on the plasma membrane. Enzyme activity is not dependent on the presence of stable homodimers As the mutations in the cytoplasmic region and the a1-helix of CD38 affected dimer stability ... homodimers (data not shown). These results suggest that the stabilization of CD38 homodimers by crosslinkers depends strongly on the conformation and orientatio...

Ngày tải lên: 23/03/2014, 12:20

... mutation only affects the thermostability of the enzyme when it combines with the E160G mutation. Why does the single E160G mutation affect the thermo- stability whereas the V198I mutation does ... the mutation at position 198. Why does the V198I mutation further increase the thermostability of the E160G mutant? Glu160 in the wild- type lactate oxidase corresponds to...

Ngày tải lên: 23/03/2014, 21:20