Báo cáo khoa học: Correlation between conformational stability of the ternary enzyme–substrate complex and domain closure of 3-phosphoglycerate kinase potx

... Substrate-assisted domain domain cooperativity FEBS Journal 272 (2005) 1867–1885 ª 2005 FEBS 1885 Correlation between conformational stability of the ternary enzyme–substrate complex and domain closure of 3-phosphoglycerate ... estimated. These correlate well with the increased stability of the ternary complex vs. the binary ones. This increased st...

Ngày tải lên: 16/03/2014, 18:20

... 5% of the DAs and 16% of the words of the entire meeting. Thus, in total we have 36 human summaries and 24 system summaries on the 6 test meetings, on which the correlation between ROUGE and ... and spoken documents, es- pecially in the meeting domain, for example, the pres- ence of disfluencies and multiple speakers, and the lack of structure in spon...

Ngày tải lên: 17/03/2014, 02:20

... the interactions between the heme and the surrounding globin, being influenced by the attached ligand and thereby by the spin state of the iron atom [11]. According to Perutz et al. [18], the region ... in the Q 0 band [25,26]. The splitting of these bands has been regarded as a lowering of the heme symmetry in HbA-CO [17,27], due to nonperpendicular iron–ligand...

Ngày tải lên: 23/03/2014, 12:20

... pocket of the I. tartaricus c-ring. Harmonic restraints were placed on the backbone atoms of the c-ring, with the intention that only the residues of the a subunit and the side chains of the c-ring ... to the outer helix of the c-ring at a dis- tance between helix axes of 12 A ˚ . The longitudinal rotation of the model helix was such that the side c...

Ngày tải lên: 16/03/2014, 06:20

... in the corresponding pH-rate profiles. The pK a values of the cofactor phosphate in unliganded EcMalP and the EcMalP–arsenate complex are 5.6 [26] and 6 .7 [27], respectively. The pK a of the ... in the presence of 5 m M potassium phosphate and potassium sulphate. Reconstitutions with PLP of apo- Cc StP and mutants thereof, and characterization of the wild...

Ngày tải lên: 19/02/2014, 16:20

... and enzyme–product complexes [29]. On the other hand, k cat ⁄ K m is an apparent second-order rate constant that refers to the properties and the reaction of the free enzyme and free substrate [29]. We have therefore ... of RNase-A in the presence of these amino acids and amino acid derivatives, and values of DG D °, measured in triplicate, are given in Table 1. T...

Ngày tải lên: 07/03/2014, 00:20

... localized to the N-terminal region, and their specific functional domains are located in the C-termi- nal region. The C-lobe of protein kinases mediates the interaction with activators, substrates and ... central domain of MAP2 contain the v-KIND binding core module MAP2 consists of three main structural domains: the cAMP-dependent protein kinase regulatory subunit RI...

Ngày tải lên: 14/02/2014, 19:20

... comparison of rhodamine uptake, in the presence and the absence of uncouplers, allows the estimation of the effect of intracellular binding on the MDR phenomenon. The data detailed obtained in the present ... with the accumulation of dyes, and probably certain drugs, in the cytoplasm. On the other hand, high-affinity binding of these agents to intracellular...

Ngày tải lên: 18/02/2014, 13:20

... involved in the interaction as the binding of 125 I-labelled DS was reduced to isogenic derivatives of the AP1 strain lacking either of these surface proteins (Fig. 2). The significance of the M1 protein ... electrostatic interactions between the negatively charged sulfate groups of the GAG chains and positively charged regions of the ligand. Typi- cally, the...

Ngày tải lên: 20/02/2014, 11:20

... energy of the cofactor is sensitive to changes in the pK a of the lower axial base [3]. This has led to speculation that proteins might modulate the pK a of the histidine via the hydrogen bond between ... site [18,19]. The correlation between the distortion of the adenosyl group and cleavage of the cobalt–carbon bond is still not clear. Although the pre...

Ngày tải lên: 07/03/2014, 04:20