Báo cáo khoa học: Characterization of cinnamyl alcohol dehydrogenase of Helicobacter pylori An aldehyde dismutating enzyme docx

... FEBS Journal 272 (2005) 1255–1264 ª 2005 FEBS Characterization of cinnamyl alcohol dehydrogenase of Helicobacter pylori An aldehyde dismutating enzyme Blanaid Mee 1 , Dermot Kelleher 2 , Jesus Frias 1 , ... class of alcohol dehydrogenase and provides the bacterium with a means of reducing aldehyde concentration within the cell. Abbreviations ADH, alcohol d...

Ngày tải lên: 16/03/2014, 18:20

... PC and increased GPC, indicating an imbal- ance between PL synthesis and degradation and, con- sequently, an increase of TG. Other studies suggest that the simultaneous accumulation of ML and ... the optimized sizes of the rectangles (areas of integration) were fixed and applied to all the spectra. Mean ± SD values calculated from 1D deconvolution and 2D integration of at least fi...

Ngày tải lên: 18/02/2014, 13:20

... foot, and the remodelling of larval tissues to produce adult tissues (i.e. the development of the gills and the production of an adult shell), which is accom- panied by significant growth of the soft ... is the mean + SE of three pools of four animals (tissues) or the mean of one pool of embryos or larva from one spawn. Expression levels are related to 100 copies of GAPDH....

Ngày tải lên: 19/02/2014, 00:20

... activity of the 5¢-flanking region of exon 1 in DNASE1. Deletion of the upstream end of the 5¢-flanking region of exon 1 from position )1386 to )231, )116, or )78 did not result in any significant change. ... Oligonucleotides Sp1 and mSp1 con- tained the wild and mutant types of Sp1 site, respectively (lanes 4 and 5). The nuclear extract was preincubated with anti-PDX-1 (lane 8) or a...

Ngày tải lên: 19/02/2014, 06:20

... Characterization of the interaction between the plasma membrane H + -ATPase of Arabidopsis thaliana and a novel interactor (PPI1) Corrado Viotti, Laura Luoni, Piero Morandini and Maria ... action of the enzyme C-terminus, but further enhances the activity of the enzyme whose C-terminus has been displaced by low pH or by FC-induced binding of 14-3-3s. Results The C-terminus of...

Ngày tải lên: 19/02/2014, 07:20

... (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254. Characterization of a Serratia ... Bis-Tris) of purified SPRK. Lanes 1 and 4: SeeBlue Ò standard (Invitrogen); Lane 2: Purified SPRK without addition of PMSF prior to SDS ⁄ PAGE analysis; Lane 3: Purified SPRK (PMSF in...

Ngày tải lên: 19/02/2014, 07:20

... overlapping fragments of chromosome XIII carrying SNO1 and SNZ1 were capable of complementing the defect of mutant K64 (Figs 1 and 2). They are homologues of PDX2 and PDX1 of fungus C. nicotianae, respectively ... of all of these genes remains to be clarified. Expression and purification of Sno1p and Snz1p In light of the similarity in the amino acid sequence of Sno1p to tha...

Ngày tải lên: 19/02/2014, 12:20

... complexity of TXA 2 signaling and provides an additional critical mechanism whereby the effects of TXA 2 can be modulated in an isoform and ⁄ or cell ⁄ tissue specific manner. The overall aim of the ... Thromboxane A2 signalling in humans: a ‘Tail’ of two receptors. Biochem Soc Trans 29, 641–654. 4 Lianos EA & Bresnahan BA (1999) Effect of thrombox- ane A2 inhibition and anta...

Ngày tải lên: 19/02/2014, 16:20

... accumulation of both anthocyanins and proanthocyanidins in Arabi- dopsis. Plant J 37, 104–114. 23 Mueller LA, Goodman CD, Silady RA & Walbot V (2000) AN9 , a petunia glutathione S-transferase required ... Scrutton and Utter plot, where k 2 and k 1 are the rate constants of the inhibition of either the bilitranslocase-ligand complex or free bilitranslocase, respectively; K d , disso...

Ngày tải lên: 20/02/2014, 01:20

... homogalac- turonan, interspersed with rhamnogalacturonan units, which often contain sugar side chains composed of arabinan and galactan [1]. Degradation of the pectin polymer occurs via a set of pectinolytic ... end of polygalacturonate. Detailed characterization of the enzyme showed that PelB is highly thermo-active and thermostable, with a melting temperature of 105 °C and a...

Ngày tải lên: 20/02/2014, 03:20