Báo cáo khoa học: Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identification of kinase substrates doc
Tài liệu Báo cáo Y học: Evidence that a eukaryotic-type serine/threonine protein kinase from Mycobacterium tuberculosis regulates morphological changes associated with cell division docx
... m M sodium orthovanadate and then assayed for phosphorylation activity. (E) Phosphoamino-acid analysis of PknA. MBP–bgal control (lanes 1 and 3) and MB P–PknA fusion protein (lanes 2 and 4) after Western-blot ... PAPER Evidence that a eukaryotic- type serine/threonine protein kinase from Mycobacterium tuberculosis regulates morphological changes associated with cell div...
Ngày tải lên: 22/02/2014, 04:20
Báo cáo khoa học: Ionizing radiation utilizes c-Jun N-terminal kinase for amplification of mitochondrial apoptotic cell death in human cervical cancer cells pptx
... from mitochondria, and apoptotic cell death. Radiation also induced transcrip- tional upregulation of Fas, caspase-8 activation, Bax and Bak activation, and phosphorylation and downregulation of Bcl-2. ... effector domain (DED) domains of the adaptor molecule, Fas-associated death domain (FADD), and caspase-8. We performed co- immunoprecitation assays to analyze the associatio...
Ngày tải lên: 07/03/2014, 05:20
Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc
... Characterization of the interaction between the plasma membrane H + -ATPase of Arabidopsis thaliana and a novel interactor (PPI1) Corrado Viotti, Laura Luoni, Piero Morandini and Maria Ida ... interactor, isoform 1 (PPI1) is a novel interactor of the C-ter- minus of Arabidopsis thaliana plasma membrane H + -ATPase (EC 3.6.3.6) (Morandini P, Valera M, Albumi C, Bonza MC, Giac...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt
... 5¢-CTTTAACTTGTTGGGCACTGG CATTG-3¢; NP6, 5¢-TTGATCGATTCTGTCTATGCCC CA-3¢ along with the adaptor primers: AP1 (5¢-GTAATAC GACTCACTATAGGGC-3¢) and AP2 (5¢-ACTATAGGG CACGCGTGGT-3¢). Nested PCR was carried ... PCR was performed in 50 lL containing 1 ng of genomic DNA as template, 0.2 mm dATP, dCTP, dGTP and dTTP, 0.2 lm of upstream primer (OP17: 5¢-GA AAAACCATGGTGAATGAATACCAAGCGACT-3¢ ) a...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx
... Aiba Y, Nakamura K, Namba T, Hirata M, Mazda O, Katsura Y & Narumiya S (1993) Thromboxane A2 receptor is highly expressed in mouse immature thymocytes and mediates DNA fragmentation and apoptosis. ... pGL3e: Prm3 AP)1 *, pGL3b: Prm 3a AP)1 *, pGL3e:Prm 3a AP)1 *, pGL3b:Prm3ab AP)1 *, pGL3e: Prm3ab AP)1 *, pGL3b: Prm3aa AP)1 *, pGL3e:Prm3aa AP)1 *, pGL3b:Prm3aaa AP)1 * and pGL3e:P...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt
... three sets of primers: first set, A5 1 (5¢- GATGTCACGCAGAGTGAGCAGGTAG-3¢)/TRHR-7 (5¢-GAGACCATACAGAAC-C-3¢); second set, A5 2 (5¢- AGAGTGAGCAGGTAGCGAGAGGAG-3¢)/TRHR-8 (5¢-GGGGGTGTAGAGGTTTCTGGAGAC-3¢); ... (5¢-ATAATGGATAA CGTAACTTTTGCTG-3¢)/TRHR1-4 antisense (5¢-TC TGTTAAATGTACCTAAGTAGGCA-3¢)andTRHR2-2 sense (5¢-CAGCAAAATGGAAAATAGTAGC-3¢)/ TRHR2-4 antisense (5¢-CGACACTGTAGTAG-AGAT CACC-3¢),...
Ngày tải lên: 21/02/2014, 03:20
Tài liệu Báo cáo khoa học: Characterization of a chemosensory protein (ASP3c) from honeybee (Apis mellifera L.) as a brood pheromone carrier pdf
... 5¢-GAGCCCGGATCCACCATGAA GGTCTCAATAATT 3¢;3¢ primer, 5¢-CTGACG GAAT TCTTAAACATTAATGCC 3¢. These primers encoded a Kozak consensus sequence as well as BamHI and EcoRI restriction sites. The PCR-amplified ... that brominated fatty acid and ASA both associated with W81 in the same ligand binding site. The ligand binding activity of ASP3c was further investigated using displacement of ASA...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae potx
... Journal compilation ª 2011 FEBS 1853 Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae Malea M. Kneen 1 , Razvan Stan 1 , Alejandra Yep 2 , ... Glu545Leu variant of ScPPDC was prepared, and its kinetic parameters with the two substrates, PPA and IPyA, were determined. A com- parison of these and the parameters of...
Ngày tải lên: 06/03/2014, 00:21
Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt
... localization and induction by highlight. Mol Microbiol 69, 231–244. 41 Prado-Cabrero A, Estrada AF, Al-Babili S & Avalos J (2007) Identification and biochemical characterization of a novel carotenoid ... ends of carotenoids. Abbreviations CCD, carotenoid cleavage dioxygenase; GST, glutathione S-transferase; NIST, National Institute of Standards and Technology; OsCCD1, Or...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Characterization of inhibitors of phosphodiesterase 1C on a human cellular system potx
... GAT CATGCACTGAAATTTA (2), GATGAAACCTCTCAA ACTG (3), and CATCATCGCTGGACAATGT (4)], using T. R. Dunkern and A. Hatzelmann Characterization of inhibitors of PDE1C FEBS Journal 274 (2007) 4812–4824 ª 2007 The Authors ... Dunkern and A. Hatzelmann Characterization of inhibitors of phosphodiesterase 1C on a human cellular system Torsten R. Dunkern and Armin Hatzelmann Bioche...
Ngày tải lên: 07/03/2014, 05:20