Báo cáo khoa học: Mapping contacts between regulatory domains of skeletal muscle TnC and TnI by analyses of single-chain chimeras potx

... FEBS Journal 272 (2005) 779–790 ª 2005 FEBS Mapping contacts between regulatory domains of skeletal muscle TnC and TnI by analyses of single-chain chimeras Ana O. Tiroli 1,2, *, Ljubica Tasic 1, * , †, ... 1.6 V[model -TnC( 1–91) TnI( 98–182)] ⁄ V (model -TnC( 1–91) TnI( 98–147) 1.3 ± 0.1 MM [TnC( 1–91) TnI( 98–182)] ⁄ MM [TnC( 1–91) TnI( 98–147)] 1.3 Contacts...

Ngày tải lên: 16/03/2014, 18:20

... observed between two atoms (i,j), of types t i and t j , and N obs (d ij ,t i ,t j ) represents the number of atoms of types t i and t j observed in the structure training set, separated by a distance ... structure. Some RRM and KH domains in complex with single strand DNA (PDB codes: 2UP1, 1WTB, 1X0F, 1ZZI and 1ZZJ) were also included in the test set because recognition...

Ngày tải lên: 18/02/2014, 16:20

... domain of MDCK cells (Fig. 2A, see top of the cell monolayer). To further confirm and quantify the steady-state distribution of GD3 and GM1 gangliosides in apical and basolateral domains of MDCK ... surface with the antibody R24 and CTx, specific for GD3 and GM1, respectively. The binding of R24 antibody and CTx in both apical and basolateral domains was evalu- ated...

Ngày tải lên: 16/03/2014, 04:20

... leakage of hemolymph and the spread of infectious pathogens, while lectins and antimicrobial peptides aggregate and lyse the patho- gens. Hemocyte exocytosis is specifically induced by LPS, but not by ... signal transducers by way of nuclear factor (NF)- jB, thus leading to the production of pro-inflammatory cyto- kines and the expression of costimulatory molecules on th...

Ngày tải lên: 30/03/2014, 20:20

... cross- fertilization of systems and resources using different semantic representations very difficult. In this paper, we present a machine learning ap- proach towards mapping between deep and shallow semantic ... seman- 189 tic predicates and their linguistic behaviour (includ- ing the set of semantic roles, indication of optional arguments, and their possible value constrain...

Ngày tải lên: 08/03/2014, 01:20

... localization and enzymatic activities of GAPDH– citrine and PGK–cerulean. (A) Expression of GAPDH–citrine and PGK–cerulean. Localization of endogenous GAPDH and PGK in HeLa cells was examined by using ... tetrameric structure of GAPDH is schematically shown on the right. The distances between the N- terminal amino acids of subunits O and P, subunits O and Q and sub...

Ngày tải lên: 16/02/2014, 09:20

... for the inhibition of elastase-2 by a 1 -PI and interference by sulfated polysaccharides. Fig. S2. Progress curves for the inhibition of elastase-2 by eglin c and interference by sulfated polysaccharides. This ... inhibition and reactivation by the con- comitant action of two modifiers I and X. Plots of the reaction rate as a function of the concentration (m M) of...

Ngày tải lên: 16/02/2014, 14:20

... m I , m t2 and m f are m-values associated with each of the identifiable states of the folding pathway and relate to the amount of solvent-exposed sur- face area in each of these states, and thus ... GCN4, a yeast homologue of AP-1, both binding and dissociation of dimers is tightly coupled with folding ⁄ unfolding of the individual helices, and is well described by a...

Ngày tải lên: 18/02/2014, 13:20

... to the standard protocols. Binding of the naturally cleaved TC fragments to mAbs Expression of TC in yeast and plants was accompanied by partial cleavage of the protein at several sites by some ... immu- nological reactivity by western blotting and identified by N-terminal sequencing. Binding of mAbs to synthetic peptides of TC Two long peptides of 30 residues (P A and...

Ngày tải lên: 20/02/2014, 01:20

... Localization Fig. 4. Mapping of Gua-binding domain in human RPL4. (A) Schematic representation of wild-type and mutant forms of human RPL4. The open bar represents regions conserved between human and mouse ... activity and GTP-stimu- lated RNA foldase activity [17–19]. The presence of both RNA unwinding and RNA folding activities in two distinct domains of the same pro...

Ngày tải lên: 20/02/2014, 01:20