Báo cáo khoa học: EspB from enterohaemorrhagic Escherichia coli is a natively partially folded protein pptx
Báo cáo khoa học: EspB from enterohaemorrhagic Escherichia coli is a natively partially folded protein pptx
... Hamada et al. 768 FEBS Journal 272 (2005) 756–768 ª 2005 FEBS EspB from enterohaemorrhagic Escherichia coli is a natively partially folded protein Daizo Hamada 1 , Tomoaki Kato 1,2 , Takahisa ... be, at least, partially folded. Of various approaches, only PONDR and DisEM- BL indicated that EspB may be natively partially folded. Incidentially, both prediction m...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Identification and functional characterization of a novel barnacle cement protein pptx
... TSVSAGDGAFGNLAAALTLVEDTEDGLGVKTKNGGKGFSEGTAAISQTAGANGGATVKKA Bacp19k VSASAANGFFKNLGKATTEVKTTKDGTKVKTKTAGKGKTGGTATTIQIADANGGVSEKSL Bicp19k AAAAAGNGVFKNLVTALTNISTTDDITKVQTQTIGSGGTGGAATILQLADANGGAALKEV 130 ... Urushida 1 , Masahiro Nakano 1 , Satoru Matsuda 1 , Naoko Inoue 2 , Satoru Kanai 2 , Naho Kitamura 3 , Takashi Nishino 3 and Kei Kamino 1 1 Marine Biotechnology Institute, Iwate, Japan 2 P...
Ngày tải lên: 16/03/2014, 05:20
Báo cáo khoa học: Functional interaction of Escherichia coli heat-labile enterotoxin with blood group A-active glycoconjugates from differentiated HT29 cells docx
... Func- tionally, differentiation was accompanied by the expression of aminopeptidase N, lactase, maltase and sucrase activities. Sucrase-isomaltase is localized at the apical brush border membranes of HT29 ... differentiated HT29 cells Estela M. Galva ´ n, German A. Roth and Clara G. Monferran Departamento de Quı ´ mica Biolo ´ gica – CIQUIBIC (CONICET), Facultad de Ciencias Quı ´ micas, Un...
Ngày tải lên: 30/03/2014, 10:20
Tài liệu Báo cáo khoa học: Catabolite repression in Escherichia coli – a comparison of modelling approaches docx
... cellu- lar networks allows the simplification of the set of equations by assuming a steady state of the intra- cellular metabolites. An approach that combines flux balance analysis (FBA) with an ordinary ... Diva environment [24] with highly sophisticated methods for sensitivity analysis, parameter analysis and parameter estimation, 50 parameters (34%) could be estimated. In particular, the a...
Ngày tải lên: 18/02/2014, 13:20
Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
... 5¢-end and 3¢-end of acs: delacs P1 (forward), 5¢-GAGAACAAAAGCATGAGCCAAATTCA CAAACACACCA TTGTG TAGGC TGGAGCT GCTTC G-3¢; and delacs P2 (reverse), 5¢-GGCAATTGTGGGTTAC GATGGCATCGCGATAGCCTGCTTCATATGAATATC CTCCTTA-3¢. ... phosphoenolpyruvate (PEP). Acetyl-CoA Acetate Pta Acetyl-AMPAcetyl-P Ack Acs Acs P i CoA ADP ATP AMP CoA PP i ATP 2P i PPasa Glucose PEP Pyruvate Acetyl-CoA Acetate CoA NADH NAD...
Ngày tải lên: 06/03/2014, 11:20
Báo cáo khoa học: Invasion of enteropathogenic Escherichia coli into host cells through epithelial tight junctions ppt
... proteins, and the appearance of aberrant TJ strands in the lateral membrane [3]. Recently, TJs have been considered as specialized plasma membrane microdomains, lipid raft-like mem- brane compartments ... several invasive pathogens including Salmonella, Shigella, Listeria and Chlamydia [5–8]. It is likely that Campylobacter jejuni also utilizes lipid rafts as the entry point [9]. Coxsackiev...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf
... available for E. coli; c value for Paracoccus denitrificans, no data available for E. coli. d value for Bacillus subtilis, no data available for E. coli; e no data available, assigned to group II based on ... Australian National University, Canberra, Australia Cell-free protein synthesis offers rapid access to proteins that are selectively labelled w ith [ 15 N]amino acids and s uitable...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Limited proteolysis of Escherichia coli cytidine 5¢-triphosphate synthase. Identification of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis potx
... 5¢-GCTGAGTCGGTTTGGTT GCGACTTCACCAGACGC-3¢ (R, K18 7A) , 5¢-CGG CAACGTTGAAGTT GCTAGCGAGAAGAGCG-3¢ (F, R42 9A) , 5¢-CGCTCTTCTCGCTA GCAACTTCAACGT TGCCG-3¢ (R,R42 9A) ,5¢-GCAACGTTGAAGTT GCTAGCGAGGCGAGCGATCTCG-3¢ ... the Triad family of glutamine amidotransferases [5,6] which utilizes a Cys-His-Glu triad to catalyse glutamine hydrolysis and also includes anthranilate synthase, carbamoyl phosphat...
Ngày tải lên: 23/03/2014, 17:22
Báo cáo khoa học: Polyphosphates from Mycobacterium bovis – potent inhibitors of class III adenylate cyclases pptx
... a HAMP domain between its membrane anchor and the catalytic domain, and CyaB1 from Anabaena sp., which has an N-terminal cAMP-binding tandem GAF domain; as a class IIIc AC, the M. tuberculosis ... [48]. Because the catalytic folds of a bacterial class IIIc and a mammalian class IIIa AC are superimposable [3,6], it is reasonable to assume that mammalian ACs will be inhibited by pol...
Ngày tải lên: 30/03/2014, 02:20
Tài liệu Báo cáo khoa học: The elusive intermediate on the folding pathway of the prion protein pptx
... two-state model, as described in the previous section. Phase-diagram analysis of fluorescence data A novel, qualitative approach to the analysis of folding data, complementary to the conventional ... PrP W175 and PrP W198 , adopt the same a- helical conformation as the wild-type protein, displaying the characteristic minima at 209 and 220 nm and a maximum at 192 nm (Fig. 2). Both variant...
Ngày tải lên: 18/02/2014, 17:20