Báo cáo khoa học: Probing the mechanism of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis of the active site pot
Tài liệu Báo cáo khoa học: Probing the molecular determinants of aniline dioxygenase substrate specificity by saturation mutagenesis docx
... be controlled by the AtdA3 subunit. The V205A mutation enables the enzyme to dihydroxylate 2IPA, a substrate not accepted by the WT enzyme, and the I248L muta- tion enhances the activity of the enzyme ... to the enzyme. This may indicate that the steric limitation of the enzyme s binding pocket takes place in the area between the ortho and para positions...
Ngày tải lên: 19/02/2014, 02:20
Báo cáo khoa học: Structure and mechanism of the ThDP-dependent benzaldehyde lyase from Pseudomonas fluorescens potx
... deprotonation of the hydroxyl by His29 followed by the dissociation of the first aldehyde. The remaining activated aldehyde is then protonated and also released. The protonation is probably performed by the ... flanked by a varying number of a-helices. Residues involved in binding of the cofactor ThDP are located at the C-terminal ends of the b-strands of D...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190 pot
... [27]. On the basis of the Ping Pong mechanism, the acetylation rate (k 2 )of NAT2 by the acetyl donor is independent of the trans- acetylation rate (k 4 ) of the arylamine substrate. Because the values ... decrease in the lifetime of the acetylated enzyme. Removal of the para-hydroxyl group by the Tyr190 to Phe mutation resulted in a decrease in the r...
Ngày tải lên: 16/03/2014, 00:20
Tài liệu Báo cáo khoa học: An ecdysteroid-inducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori docx
... achieved. Determination of the structure of 8K-BLP MALDI-TOF MS analysis of the purified material from the final HPLC yielded a monoisotopic mass of 7409.2 ([M + H] + ) (Fig. 3A). Amino acid sequence analysis of the ... to those of bombyxin and insulin, which are of the order of 100 pm [23,24]. The very high titer of 8K-BLP may be due to a remarkably high level of...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx
... 1.10.3.1), but the differentiation between these two types of enzyme is not clear [4]. Looking at the sequences of the two enzymes, both show absolute conservation of the histi- dine residues of the CuA ... disproportion of dopaquinone can be titrated at different periods of time by addition of sodium perio- date. According to the high preference of the enzy...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: "Discovering Global Patterns in Linguistic Networks through Spectral Analysis: A Case Study of the Consonant Inventories" pdf
... correspond- ing to the largest eigenvalue) is the direction in which the sum of the square of the projections of the row vectors of the matrix is maximum. In fact, the principal eigenvector of a graph ... three taken together. The individual contribution of the other eigenvalues to the spectrum is significantly lower than that of the top three. Third, the...
Ngày tải lên: 22/02/2014, 02:20
Tài liệu Báo cáo khoa học: "Probing the lexicon in evaluating commercial MT systems Martin" pot
... content of the translations, the translation quality, and the user-friendliness. The list of criteria for self evaluation consisted of technical, linguistic and ergonomic issues. As part of the ... obtained the triples in ta- ble 1. The first number in a triple is the percentage of positive counts in the high frequency class, the second number is the percent...
Ngày tải lên: 22/02/2014, 03:20
Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx
... immunomodulators to reveal the mechanism of action of toxic MDPs on mitochondrial respiration by correlating the uncoupling effect induced by these molecules with the level and function of UCP2 and free ... [28]. These findings agree with the hypothesis [29] that an increase in the mitochondrial membrane potential would slow the transport of elec- trons through the...
Ngày tải lên: 05/03/2014, 23:20
Báo cáo khoa học: A novel mechanism of V-type zinc inhibition of glutamate dehydrogenase results from disruption of subunit interactions necessary for efficient catalysis doc
... less of the enzyme is involved in productive enzyme NADPH complexes involved in the overall rate limiting step of the reaction. The effects of zinc on the binding of reduced cofac- tor to the enzyme ... observed. Effects of zinc on the stability of the enzyme The thermal stability of the enzyme was determined using differential scanning calorimetry i...
Ngày tải lên: 05/03/2014, 23:20
Báo cáo khoa học: Protein aggregation and amyloid fibril formation prediction software from primary sequence: towards controlling the formation of bacterial inclusion bodies pot
... of a4v over the entire sequence defines the aggregation profile of the polypeptide. The hot-spot threshold (HST) was defined as the average of the a3v of the 20 natural amino acids weighted by their ... central residue of the window. The ‘smoothed’ expected values for every position of the polypeptide chain provide the final profile, which is directly used for...
Ngày tải lên: 06/03/2014, 00:20