Báo cáo khoa học: Cloning, over-expression, purification and characterization of Plasmodium falciparum enolase doc

... component enolase. J. Mol. Biol. 313, 5 83–592. 4854 I. Pal-Bhowmick et al.(Eur. J. Biochem. 271) Ó FEBS 2004 Cloning, over-expression, purification and characterization of Plasmodium falciparum enolase Ipsita ... repo rts of antibodies to enolase detected in high titers in Japanese and Thai P. falciparum patient sera and use of yeast enolase for immunodiagnost...

Ngày tải lên: 16/03/2014, 18:20

... targets, the reconstruction of pathways and help to understand the mechanism of action and side-effects of therapeutic compounds. Correspondence to B. Kuster, Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, ... activity of the spliceosome is the recognition of splice sites in nuclear pre-mRNA and to catalyze the accurate removal of introns and ligation of exons to yiel...

Ngày tải lên: 31/03/2014, 07:20

... melting curves of wild-type and mutants of SNase. DSC curves of five proteins [wild-type (WT), W140A, E140O, W140O and K133A]. The curves of W140A and W140O are nearly linear in terms of intensity. ... 3961 of the wild-type protein and K133A and E142O were 88.45, 84.70, and 83.60 kcalÆmol )1 , respectively. The melting point and DSC profile of E142O (52.8 °C) was alm...

Ngày tải lên: 20/02/2014, 01:20

... difference of DH cal of mutant from wild-type (%), heat capacity (DC p ) and difference of DC p of mutant from wild-type (%) were determined and the results for the wild-type protein and 20 mutants ... Calometric melting curves of wild-type and mutants of SNase. DSC curves of nine proteins (WT, E73G, E75G, E101G, E122G, E129G, D77G, D83G and D95G). Lines 3 and 6 repr...

Ngày tải lên: 07/03/2014, 21:20

... Spanish and vice versa (per- forming 5 iterations of model IBM1 and HMM, and 3 iterations of models IBM3 and IBM4), and have evaluated two symmetrization strategies, namely the union and the ... |S| 70 where A is the hypothesis alignment and S is the set of Sure links in the gold standard reference, and P includes the set of Possible and Sure links in the gold standa...

Ngày tải lên: 17/03/2014, 06:20

... = y} and F(y) =  ω∈Ω(y) {f ∈ ω}. That is, Ω(y) is the set of parses of a string y and F(y) is the set of features appearing in the parses of y. In the gram- mars of interest here Ω(y) and hence ... value that maximises a product of functions or else as the sum of a product of functions, each of which depends on a small subset of the variables X. These are the kinds...

Ngày tải lên: 31/03/2014, 06:20

... 1992; Riloff and Jones, 1999; Thelen and Riloff, 2002; Pantel and Pennacchiotti, 2006), named entity recognition (Collins and Singer, 1999), part -of- speech tagging (Clark et al., 2003), and statistical ... size of the stop list is set to n stop = 10, and the number of seeds n seed = 10 and iterations τ = 20. For both HITS and NoStop, the seeds are selected at random from...

Ngày tải lên: 20/02/2014, 04:20

... submitted to MALDI- TOF analysis and assigned to distinct subunits. Fig. 1. SDS/PAGE and immunoblotting of S. coleoptrata hemocyanin. About 10 lg of total hemolymph protein (HL) and 3 lgofpurified hemocyanin ... composed of 12 HcA, 12 HcB, six HcC and six HcD polypeptides. Thus the basic building block of the hemocyanin, the hexamer, most likely contains two copies of each Hc...

Ngày tải lên: 20/02/2014, 11:20

... amount of work in Natural Language Processing (NLP) using measures of semantic similarity and relatedness. We believe that the extensive usage of such measures derives also from the availability of ... download, and can be accessed using robust Open Source applications, e.g. the MediaWiki software 5 , integrated within a Linux, Apache, MySQL and PHP (LAMP) software bundle. The a...

Ngày tải lên: 20/02/2014, 12:20

... the work of Grote (1998), Grote et al. (1997), Jayez and Rossari (1998) and Lagerwerf (1998). Secondly, the approach demands an understand- ing of the attentional characteristics of presupposi- ... for NL understanding and/ or gener- ation. But the work to date surely shows the benefit of an approach that narrows the gap between dis- course syntax and semantics and tha...

Ngày tải lên: 20/02/2014, 18:20