Báo cáo khoa học: CpG methylation of the CENP-B box reduces human CENP-B binding pptx

... on the sixth position of the cyto- sine, which leads to the formation of a covalent bond between the enzyme and the substrate base. The for- mation of the cysteine–cytosine bond increases the ... because the DG° for the transfer of the methyl group from AdoMet to cysteine is of the order of )70 kJÆmol )1 [2,4]. Therefore, it is essential for the enzyme th...

Ngày tải lên: 14/03/2014, 23:20

... inhibi- tion of the binding of the superoxide substrate and the other to product inhibition by hydrogen peroxide. There are two mechanisms proposed for the reaction cycle of Mn ⁄ Fe-SOD: the 5-6-5 ... ligand, NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions. The manga- nese was only 0.06 A ˚ out of the equatorial p...

Ngày tải lên: 14/02/2014, 22:20

... man- ganese in the transcriptional regulation of the nrd operon [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo ... to the catalytic site of the R1E subunit. As reported in the Results, upon completion of substrate conversion, the radical is then rapidly passed back from...

Ngày tải lên: 15/02/2014, 01:20

... PhyK, presumably making the catalytic pocket more rigid. The N-terminus of this elongated helix points towards the substrate -binding site. Thus, the dipole moment of the helix supports the binding of negatively ... well. The hydrogen bond between the backbone nitrogen atom of Asp291 and the 3- phosphate of the substrate is the only interaction with the pr...

Ngày tải lên: 16/02/2014, 09:20

... EC 2.7.2.4) is an enzyme that catalyzes the first committed step, the phosphorylation of the c-carboxyl group of aspartate, of the biosynthetic pathway of the aspartic acid group amino acids Lys, Thr, ... Thr binding shifts the equilibrium to dimer formation. In the absence of Thr, an outward shift of b-strands near the Thr -binding site (site 1) and a concomitan...

Ngày tải lên: 18/02/2014, 08:20

... that the activation of IRF-3 by M-pIC treatment is mediated by the TLR3 ⁄ TRIF signaling pathway. We obtained further evidence by examining the status of the phos- phorylation and dimerization of ... cells. First, we confirmed the effect of NS3-4A on the activation of the IFN-b gene promoter by the Cardif exogenously expressed in PH5CH8 cells. The results of the...

Ngày tải lên: 18/02/2014, 16:20

... fitting to the equation: k obs ¼ k 1 ½Eþk À1 : Measurement of the dissociation constant, K d P of the ribozyme–product complex The equilibrium dissociation constant K d P of the 5¢ exon mimic binding ... K d is the equilibrium dissociation constant of the 5¢ exon mimic, h is the fraction of 5¢ exon mimic bound to the ribozyme, and [ribozyme] u is the concentrat...

Ngày tải lên: 18/02/2014, 18:20

... according to the residue in the mid- point of the respective loop, as shown in Fig. 2. To the east of the active site the 37- and 60-loops border the S2¢ pocket of the proteinase. The observed ... below). The southern boundary of the active site cleft of DESC1 is formed by the 145 autolysis loop. The backbone of this loop differs mark- edly from the oth...

Ngày tải lên: 19/02/2014, 00:20

... determines the shape of the active site entry. (e) The structure of the active site is essentially identical with the active sites of the MshB and LpxC proteins. The conservation of catalytically important ... justifies the presence of acetate in the crystal structure. The binding of acetate in the active site is a further indication that the enzyme may be...

Ngày tải lên: 19/02/2014, 00:20

... amplitudes and rate constants of the two other phases. The above should be gov- erned by the balance between the rate of reduction and oxidation of the acceptor side of PSII by the avail- able electron ... when the temperature is raised. The effect of DCMU on the traces was to increase the amplitude of step J with the concurrent decline of step I, and to re...

Ngày tải lên: 19/02/2014, 05:20