Báo cáo khoa học: CpG methylation of the CENP-B box reduces human CENP-B binding pptx
Báo cáo khoa học: Auto-methylation of the mouse DNA-(cytosine C5)-methyltransferase Dnmt3a at its active site cysteine residue pot
... on the sixth position of the cyto-sine, which leads to the formation of a covalent bondbetween the enzyme and the substrate base. The for-mation of the cysteine–cytosine bond increases the ... because the DG° for the transfer of the methyl group from AdoMet to cysteineis of the order of )70 kJÆmol)1[2,4]. Therefore, it isessential for the enzyme that a close approximation of these ... dsDNA with the standard auto -methylation reaction. Whereas Dnmt3L increases the level of auto -methylation of Dnmt3a-C, the methylation of the dsDNA substrate is efficientlycompeting with the Dnmt3a-C...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... inhibi-tion of the binding of the superoxide substrate and the other to product inhibition by hydrogen peroxide.There are two mechanisms proposed for the reactioncycle of Mn ⁄ Fe-SOD: the 5-6-5 ... ligand,NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions. The manga-nese was only 0.06 A˚out of the equatorial plane(Table 3). The angles around the metal ... excluded all residuesinvolved in the binding of the metal cofactor.ApeSODs in different forms were superimposed with the least square fit of Ca atoms of the residues in the range10–200. ApeSOD and...
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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... man-ganese in the transcriptional regulation of the nrdoperon [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo ... to the catalytic site of the R1E subunit. Asreported in the Results, upon completion of substrateconversion, the radical is then rapidly passed backfrom the R1E subunit to the tyrosyl of the ... RNR and that of other class 1RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E.We consider the first option unlikely. Under these cir-cumstances,...
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Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc
... PhyK,presumably making the catalytic pocket more rigid. The N-terminus of this elongated helix points towards the substrate -binding site. Thus, the dipole moment of the helix supports the binding of negatively ... well. The hydrogen bond between the backbone nitrogen atom of Asp291 and the 3-phosphate of the substrate is the only interaction with the protein backbone; all other contacts are formedusing the ... Thus, the differences between wild-typeand mutant structure are in the same range as the dif-ferences between the two molecules of the asymmetricunit of the wild-type structure. Neither the...
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Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf
... EC 2.7.2.4) is an enzyme thatcatalyzes the first committed step, the phosphorylation of the c-carboxyl group of aspartate, of the biosyntheticpathway of the aspartic acid group amino acids Lys,Thr, ... Thr binding shifts the equilibrium to dimer formation. In the absence of Thr, an outwardshift of b-strands near the Thr -binding site (site 1) and a concomitant loss of the electron density of the ... near the Thr- binding site are observed. The mechanism of regulation by Thr is discussedon the basis of the crystal structures. TtAKb has higher thermostabilitythan the regulatory subunit of...
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Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx
... that the activation of IRF-3 by M-pIC treatment is mediatedby the TLR3 ⁄ TRIF signaling pathway. We obtainedfurther evidence by examining the status of the phos-phorylation and dimerization of ... cells. First, weconfirmed the effect of NS3-4A on the activation of the IFN-b gene promoter by the Cardif exogenouslyexpressed in PH5CH8 cells. The results of the lucif-erase reporter assay revealed ... sup-press the activation (1000-fold induction) of the IFN-bgene promoter by TRIF (Fig. 7A). This result was alsosupported by the results of the dimerization analysis of IRF-3 (Fig. 7B). Furthermore,...
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Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx
... fitting to the equation:kobs¼ k1½EþkÀ1:Measurement of the dissociation constant, KdP of the ribozyme–product complex The equilibrium dissociation constant KdP of the 5¢ exonmimic binding ... Kdis the equilibrium dissociation constant of the 5¢ exon mimic, h is the fraction of 5¢ exon mimic boundto the ribozyme, and [ribozyme]uis the concentration of unbound ribozyme in the reaction.Measurement ... preventing the second reactionstep (exon ligation). In addition, the product of the first reaction step is actually the intermediate in the fullTES reaction.Substrate binding The rate constant for the...
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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx
... according to the residue in the mid-point of the respective loop, as shown in Fig. 2. To the east of the active site the 37- and 60-loops border the S2¢ pocket of the proteinase. The observed ... below). The southern boundary of the active site cleft of DESC1 is formed by the 145autolysis loop. The backbone of this loop differs mark-edly from the other serine proteinases, making the act-ive ... reviews[1,2,10].S1 The following segments border the S1-specificity pocket of DESC1: Asp189–Gln192 (the basement of the pocket), Ser214–Gly219 (the entrance frame), Lys224–Tyr228 (the back of the pocket) and the...
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Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc
... determines the shape of the active site entry. (e) The structure of the active site isessentially identical with the active sites of the MshBand LpxC proteins. The conservation of catalyticallyimportant ... justifies the presence of acetate in the crystalstructure. The binding of acetate in the active site is afurther indication that the enzyme may be involved indeacetylation because acetate is one of ... formation. Sections (6 A˚thick) of the protein surface that illustrate the shape and size of the active site. The sphere represents the zinc ion. (A) The main body of the active site cavity is formed...
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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc
... amplitudes and rate constants of the two other phases. The above should be gov-erned by the balance between the rate of reductionand oxidation of the acceptor side of PSII by the avail-able electron ... when the temperature is raised. The effect of DCMU on the traces was to increase the amplitude of step J with the concurrent decline of stepI, and to retard the rise to Fm. With dPQ, the J ... correspond to the end of the J–I phase [8,28,29]. It is likely that the emergence of a peak for I in the FI curves depends on the relative amplitude and rate constant of the J–Iphase compared to the amplitudes...
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Từ khóa: tài liệu báo cáo khoa học bản chất của khủng hoảng kinh tế thế giới pdflam the nao de tom tat bao cáo khoa hocbáo cáo khoa họcbáo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngThơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXTăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ