Báo cáo khoa học: Selective modulation of protein C affinity for EPCR and phospholipids by Gla domain mutation pdf

... (Phe4, Gla7 , Leu8, Gla2 5 and Gla2 9) and EPCR (Leu82, Table 3. Interaction of protein C Gla domain variants with recom- binant wildtype sEPCR. Affinity of protein C Gla domain variants for sEPCR was ... n M). (C) Complete EPCR ⁄ protein C binding cycle. 1, Wildtype sEPCR (800 ng) was injected across the flow cell of a CM5 sensor chip coated with RCR-2....

Ngày tải lên: 16/03/2014, 18:20

... (1995) Comparison of and limits of accuracy for statistical a nalyses of vibrational and e lectronic circular d ichroism spectra in terms of correlations to and predictions o f protein sec- ondary ... between transmembrane helices and peripheral helices by the deconvolution of circular dichroism spectra of membrane proteins. In Circular Dichroism and the Conformati...

Ngày tải lên: 16/03/2014, 18:20

... bis(phosphonodichloromethyl)phosphi- nic acid, pCCl 2 pCCl 2 p free acid. Synthesis of adenosine-5’-[b ,c, c,d-bis(dichlorometh- ylene)]tetraphosphate, pCCl 2 pCCl 2 ppA. Synthesis of a,b;b ,c- bis(methylene)-ATP, ... b ,c- methyleneATP. Neither of these explanations accounts for the much reduced activity of pppCH 2 pA for the human p 4 A Fig. 3. Time course of p 4 A (A) and p...

Ngày tải lên: 16/03/2014, 14:20

... 4.3 and 8, in the presence of allosteric effectors, and at pH greater than 8, in the absence of ligands, HSA changes conformation to the basic (B) form with loss of a-helix and an increased affinity ... protons, 17 O nuclei are negligibly affected by dipolar coupling with nearby unpaired electrons, and the paramagnetic broadening of the 17 O resonance is diagnostic of the...

Ngày tải lên: 20/02/2014, 02:22

... Plasmids for the production of recom- binant protein were constructed by a PCR, where forward and reverse primers encoded the desired parts of the C- and N-termini of GIRK1. These sequences were each ... regulated by cytosolic injections of PKA activators and inhibitors, suggesting it to be the basis for the functional effects of cAMP injections on GIRK currents. PK...

Ngày tải lên: 07/03/2014, 00:20

... sterically restricted arrangement of four hydrophobic amino acids and a basic amino acid is essential for plactin activity [2]. Therefore, we speculated that coupling of plactin-14 to CNBr-activated ... ue L eh P HN H N 2 N H NH HN HN N H N H O O O O O HN 2 syL HN HN 2 N H NH HN HN N H N H O O O O O N H S O O N S N D - s yL 0 1.0 2.0 3.0 4.0 u- PA activity (fluorescence intensity) aX...

Ngày tải lên: 07/03/2014, 00:20

... precursor protein; CHO, Chinese Hamster Ovary; Cp, ceruloplasmin; CQ, clioquinol; CNS, central nervous system; COX, cytochrome c oxidase; CSF, cerebrospinal fluid; Cu ⁄ Zn-SOD, copper ⁄ zinc superoxide dismutase; ... R, Duce JA, Ciccotosto GD, Li QX, Barnham KJ, Curtain CC, Cherny RA, Cappai R, Dyrks T et al. (2005) Copper-dependent inhibition of human cytochrome c oxidase by a dimeri...

Ngày tải lên: 07/03/2014, 10:20

... The idea of evaluating utterance choices in rela- tion to task success can be traced back to Ander- son and Boyle (1994) who linked utterance choic- es to the accuracy of the route performed on ... automatic calculated as number of heart beats per minute. Galvanic Skin Conduc- tance (SK) was recorded with Ag AgC1 elec- trodes attached to the palmar surface of the second and thi...

Ngày tải lên: 08/03/2014, 01:20

... native coding sequence. External primers 5¢-GAAGATCTGACTACAA GGACGACGATGAC-3¢ (sense) and 5¢-CCTCTAGAT TACTAACATTTCTTCAACAAGCATTG-3¢ (antisense) containing a BglII and XbaI restriction site, respectively, were ... pIB/V5-His/TOPOÒ vector using two primers, 5¢-CC GATCTCAATATGAAGTTATGC-3¢ (sense) and 5¢-CCT CTAGATTACTAACATTTCTTCAACAAGCATTG-3¢ (antisense) and the TOPO TA cloningÒ methodo...

Ngày tải lên: 08/03/2014, 02:20

... and some of the discus=dons of the heat of re(retry, but the critical details are completely out of reach. Rather than carefully controlled evaluations, the public needs competent and honest ... SELECTIVE PLANNING OF INTERFACE EVALUATION~ William C. Mann USC Information Sciences Institute 1 The Scope of Evaluations The basic ides behind evaluation is 8 simple one: A...

Ngày tải lên: 08/03/2014, 18:20