Báo cáo khoa học: Probing the unfolding region of ribonuclease A by site-directed mutagenesis potx

... TGT AAC CAG ATG ATG GC G AGC TCG AAC CTG ACC AAA GAT C3¢ SacI rev 5¢-G ATC TTT GGT CAG GTT C GA GCT CGC CAT CAT CTG GTT ACA G-3¢ L3 5A fw 5¢-G ATG ATG AAG AGC CG GAAT GCC ACC AAA GAT CGA TGC AAG ... revealed that all RNase A variants are active (Table 3). However, while the RNase A variants with mutations in the Ala20 loop region a s well as N 34D- RNase A and L3 5A- RNase A...

Ngày tải lên: 16/03/2014, 18:20

... BL21(DE3) Activity against 2MA atdA1 pACYC A2 and pET A3 A 4A5 – atdA2 pACYC A1 and pET A3 A 4A5 + atdA3 pACYC A1 A2 and pET A4 A5 – Control (no deletion) pACYC A1 A2 and pET A3 A 4A5 + E. L. Ang et al. Substrate ... and A1 _SalI_R primers. The atdA2 gene was amplified using the A2 _FseI_F and A2 _AvrII_R primers. The atdA3 gene was amplified using the A3 _EcoRI_F and A3 _S...

Ngày tải lên: 19/02/2014, 02:20

... with p-nitrophenyl acetate (PNPA) ⁄ PABA Because the Y190 mutants are correctly folded, as shown by CD, the specific activity was determined as the transacetylation reaction rate of PNPA ⁄ PABA catalyzed by wild-type ... the significant decrease in the observed rate of acetylation. pH dependence of transacetylation by NAT The pH dependence of transacetylation of PNPA...

Ngày tải lên: 16/03/2014, 00:20

... preventing inactivation of bacterial a- glucan phosphorylases. Biocatal Biotrans- form 19, 379–398. 46 Griessler R, D’Auria S, Tanfani F & Nidetzky B (2000) Thermal denaturation pathway of starch ... in the H33 4A mutant through removal of the imidazole group of the His, thereby effectively replacing the function of the origi- nal side chain in catalysis by the m...

Ngày tải lên: 23/03/2014, 07:20

... nm, often decorated with stain, as well as clustered deposits of fibrils with an average diameter of 6 nm (Fig. 6C). FX1RP Nt-NES aggregates have a curved appearance, with an apparent average diameter ... the conformational transition. Relatively small variations of protein concentration also lead to an increase of the rates at which the con- formational transition occurs. An a...

Ngày tải lên: 14/03/2014, 23:20

... this accuracy. Besides, the result for flat labels compares favorably with the state of the art accuracy of about 93% F 1 for joint word segmen- tation and part -of- speech tagging (Jiang et al., ... p) (9) The other probabilities can be estimated in the same way. 4.4 Summary of the Generative Story We make a brief summary of our generative story for the integrated morp...

Ngày tải lên: 17/03/2014, 00:20

... Personal Transla- tor again ranks among the systems with the widest coverage while German Assistant shows the smallest coverage. 4 Conclusions As more translation systems become available there ... class 49 adjectives, 53 nouns, and 61 verbs got a translation. The average is computed as the mean value over the three word classes. Comparing the systems' averages we...

Ngày tải lên: 22/02/2014, 03:20

... the effects of mutations that retain and those that alter the charge. It may be significant that none of these amino acid side-chains make contact with the carbon substrate or the metal ion cofactor ... suggest that reduction occurs at the level of an isomerase transition state rather than after formation of the 2-ketoacid (Fig. 5). A similar proposal was made by Arfin &...

Ngày tải lên: 16/03/2014, 18:20

... to examine the impact of mutation and labeling of sTF on FVIIa binding and enhancement of FVIIa activity. The ability of the IAEDANS-labeled sTF variants to stimulate FVIIa was assessed and the ... the lower part of the protease domain of FVIIa and the N-terminal domain of TF to the c-carboxyglutamic acid (Gla)-rich FVIIa module and the C-terminal part of TF...

Ngày tải lên: 17/03/2014, 03:20

... inhibitor, pepstatin, bestatin and E-64 were from Roche Diagnostics (Laval, Canada). EDTA, ortho-phenanthroline and heparin were from Sigma-Aldrich (Oakville, Canada). a 1 -AT, a 1 -ACT, AT III, PAI-1 and a 2 -AP ... 569–571. 19 Kazama Y, Hamamoto T, Foster DC & Kisiel W (1995) Hepsin, a putative membrane-associated serine protease, activates human factor VII and initiates a pat...

Ngày tải lên: 23/03/2014, 04:21