Báo cáo khoa học: Investigation of the substrate specificity of a b-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis pdf

... Val216 decides the substrate specificity of a- glucosidase in Saccharomyces cerevisiae Keizo Yamamoto 1 , Akifumi Nakayama 2 , Yuka Yamamoto 1, * and Shiro Tabata 1 1 Department of Chemistry, Nara ... purchased from Takara Syuzo and restriction endonucleases and T4 DNA ligase were from Takara S yuzo, or New En gland Biolabs. Reverse transcriptase was used from the Expa...

Ngày tải lên: 07/03/2014, 16:20

... of the associated polymerase domain. In the internal cleavage mode, the RNases H behave as typical endo- nucleases and cleave the RNA along the length of a DNA ⁄ RNA hybrid substrate in the absence ... retroviral RNases H. DNA ⁄ RNA hybrids are drawn with RNA strands in red and DNA strands in black. In the internal cleavage mode, the arrows mark the sites of cl...

Ngày tải lên: 30/03/2014, 02:20

... instru- ment from Microcal (Northampton, MA, USA). The ther- mal denaturation of Cel7 4A was completely irreversible, and no transition was seen in a repeat scan; therefore, an apparent T m was approximated ... MA, USA). The midpoint of the transition (T m )isan apparent value, as the thermal denaturation was not rever- sible and was accompanied by precipitation. DSC ther...

Ngày tải lên: 19/02/2014, 05:20

... Investigation of the substrate specificity of a b-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis Sandro R. Marana, Eduardo H. P. Andrade, ... designated 4 and 6, where ÔeÕ stands for an equatorial hydroxyl and a for a n axial one. Therefore, the interaction between any residue at position 39 and an e...

Ngày tải lên: 16/03/2014, 18:20

... peptide substrates was used to assess the specificity of the A1 69L and K22 0A mutants (Table 2). In general, the mutant enzymes suffered a substantial loss of catalytic power, but they retained a mainly ... to influence the k cat values calculated from the total pro- tein content. The changes in K m for the mutants, together with the specificity alterations sug...

Ngày tải lên: 19/02/2014, 16:20

... and the supernatant used for the analysis. The protein content of the extract was estimated using the Bio-Rad protein assay, and c-globulin was used as a standard. Enzyme purification and assays To ... Foun- dation. SK was supported by the Maj and Tor Nes- sling Foundation and PR was an Academy Research Fellow of the Academy of Finland. References 1 Baliga BS...

Ngày tải lên: 19/02/2014, 06:20

... residue, the wild-type cDNA was amplified using the same upstream primer as for the entire cDNA (5¢-CGGGGATCCATGGTCTGCAAG-3¢) but 5¢-ACGCGTCGACCTAAGCAGCCTG-3¢ as the down- stream primer. All the PCR ... complexes The S, Zn, Cd and Cu content of the Zn–, Cd– and Cu–CeMT preparations was analyzed by means of ICP- AES in a Polyscan 61E (Thermo Jarrell Ash, Franklin, MA,...

Ngày tải lên: 07/03/2014, 02:20

... parser, and a matching pattern with a part of speech rather than an actual word on one side can be regarded as a relaxation rule, in the sense that syntactic and se- mantic constraints are ... what information is available about the AS/400 system. Candidates AJ N V AJ AJ DET N N for the POS AV AV of each word PN PP Phrases what information is available about the...

Ngày tải lên: 08/03/2014, 07:20

... yellow, acarviose transferase; pink, maltogenic a- amylase; blue, a- amylases from Bacillus and actinomycetes; light blue, a- amylases from fungi and yeast; green, maltotetraohydrolases and maltopentaohydrolase. ... e.g. the a- amylases produced by streptomycetes or fungi, and the CGTases from the genera Bacillus and Thermoanaerobacter (Fig. 3A) . The a- amylase...

Ngày tải lên: 08/03/2014, 08:20

... (5¢-CCGATATCATACTCTTCC-3¢)andBL(5¢-CC GATATCAGACCAAGTTTAC-3¢).Inthesameway ,the zeo gene was amplified from plasmid pHook (Invitrogen) using primers Z1 (5-CCGATATCGTGTTGACAATT AATC-3¢)andZ2(5¢-CCGATATCCAGACATGATAA GATAC-3¢). ... PEG 8000 and 50 m M NaCl, and the integration mixture was incubated at 37 °Cfor90min. Gel analysis of the integration reaction For gel analysis of...

Ngày tải lên: 23/03/2014, 15:21