Báo cáo khoa học: G protein-coupled receptor 30 down-regulates cofactor expression and interferes with the transcriptional activity of glucocorticoid pdf
Báo cáo khoa học: G protein-coupled receptor 30 down-regulates cofactor expression and interferes with the transcriptional activity of glucocorticoid pdf
... sites. The following primers were used for cloning at the pLEGFP-N1 vector: GPR30-forward 5¢-TAATAAGTCGACGGGTC TCTTCCT-3¢ and GPR30-reverse 5¢-ATTATTGGATC CTACACGGCACTGC-3¢. V iruses capable of i ... for gene delivery. GPR30 inhibited the growth and proliferation of the cells. They e xpressed glucocorticoid receptor, but not estrogen or progesterone receptor. GPR30 down-re...
Ngày tải lên: 16/03/2014, 18:20
Tài liệu Báo cáo khoa học: Agrobacterium tumefaciens type II NADH dehydrogenase Characterization and interactions with bacterial and thylakoid membranes ppt
... FAD-con- taining proteins were observed, confirming the nature of the cofactor [19]. By using the extinction coeffi- cient at 450 nm we calculated that the stoichiometric ratio of flavinic cofactor per mole of ... [38] suggested that the presence of an acidic residue (E or D) at the end of the second b sheet of the dinucleotide-binding site would confer NADH speci...
Ngày tải lên: 19/02/2014, 06:20
Báo cáo khoa học: Two conserved domains in regulatory B subunits mediate binding to the A subunit of protein phosphatase 2A pdf
... signi®cant alignment with other B subunits, homology with B/B55 and P R72 family members was found when only the B56 binding domains were used in the a lignment ( Fig. 3). For ASBD 1, the region of ... synthesized in the reticu locyte lysate (Fig. 1A), suggesting the C subun it present in the reticulocyte lysate may contribute t o the formation of heterotrimers [27]. T...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo khoa học: Hepatocyte growth factor activator (HGFA): molecular structure and interactions with HGFA inhibitor-1 (HAI-1) doc
... hydrogen bonds between P1 Arg and Ser214 and between P3 Gln and Gly216 (Fig. 3A). S1 is filled with the P1 Arg, which engages in standard salt bridge interactions with HGFA Asp189, located at the ... the ranges among close homologs. Additionally, some of these loops (the 140-, 180-, and 220-loop) undergo con- formational rearrangements during the zymogen to enzyme transi...
Ngày tải lên: 15/03/2014, 11:20
Báo cáo khoa học: Nucleosome positioning in relation to nucleosome spacing and DNA sequence-specific binding of a protein doc
... FEBS urea gels (footprinting) were generated from the Phosphori- mager using the image gauge program of Fuji (Tokyo, Japan). All protections were ascertained by matching the profiles of the lanes with ... precision would decrease with distance from the boundary [28]. We have analyzed the effect of changing the ionic strength and the number and spacing of the b...
Ngày tải lên: 16/03/2014, 10:20
Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... p47 phox to the plasma membrane. Thus, we suggest that the O 2 – -scavenging activity of selenoureas and selenoamides was not due to the inhi- bition of NADPH oxidase in PMNs. We assessed the metabolism ... USA) following the procedure recommended by the manufacturer. The purity of PMNs was > 98% as deter- mined by Randolph’s stain. Assay of effects on scavenging...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Tryptophan 243 affects interprotein contacts, cofactor binding and stability in D-amino acid oxidase from Rhodotorula gracilis ppt
... Glu322), con- necting b-strands F5 and F6 and the negatively charged residues belonging to the a-helices I3¢ and I3¢¢ of the other monomer and (b) the interactions in the core of the dimer (where ... hydrophobic regions during the urea- induced unfolding of W243 mutants compared with the wild-type DAAO. Kinetics of reconstitution of apoprotein with FAD Be...
Ngày tải lên: 30/03/2014, 11:20
Báo cáo khoa học: "Smaller Alignment Models for Better Translations: Unsupervised Word Alignment with the 0" potx
... Jason Riesa for providing the Arabic-English and Chinese-English hand-aligned data and the alignment visualization tool, and Chris Dyer for the Czech-English hand- aligned data. This research ... translations (i.e., the size of the lexical weighting table) is reduced. The ˜ φ sing. column shows the average fertility of once-seen source words. For Czech-English, the y...
Ngày tải lên: 30/03/2014, 17:20
Báo cáo khoa học: G protein-coupled receptor-induced Akt activity in cellular proliferation and apoptosis pptx
... both directly, through the interaction of Gbc subunits with PI3K, and indirectly, through the GPCR transactiva- tion of RTKs and integrins. We shall also examine the downstream signaling and physiological ... activation ⁄ inhibition of adenylyl and guanylyl cyclases, and the opening and closing of ion channels. In this review, we shall investigate the ability...
Ngày tải lên: 16/03/2014, 05:20
Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
... Protease-acti- vated receptors 1 and 4 do not stimulate Gi signaling pathways in the absence of secreted ADP and cause human platelet aggregation independently of Gi signal- ing. Blood 99, 3629–3636. 14 ... 3 integrin activation and platelet aggregation [18]. We and others have shown that both the PI3-Kb and PI3-Kc isoforms contribute to the P2Y 12 -mediated stabilization...
Ngày tải lên: 18/02/2014, 16:20