Báo cáo khoa học: X-ray structure of peptidyl-prolyl cis–trans isomerase A from Mycobacterium tuberculosis potx

... from M. tuberculosis DNA strain H37Rv [11] using t he primers 5¢-ATGGCAGACTGTGATTC CGTGAC-3¢ (forward) and 5¢-CTAGGAGATGGTG ATCGACTCG-3¢ (reverse), and Taq DNA polymerase (Roche). An additional PCR ... Measurements were made every 0.5 s during 3 min. The final absorbance was estima- ted from e ach curve; the absorbance at each time point was subtracted from that value. A plot of th...

Ngày tải lên: 16/03/2014, 18:20

... structure of Ras at the air–water interface was analysed. With the largely increased signal-to-noise ratio of ATR-FTIR, we have, for the Table 1. X-Ray and NMR-based secondary structure of Ras ... filtra- tion, using AmiconÒ concentrators. All protein batches were analysed by SDS-PAGE and MALDI-TOF-MS. Preparation of the ATR crystal The germanium IRE of the ATR was cleaned chem...

Ngày tải lên: 07/03/2014, 04:20

... ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases Juha P. Kallio 1 , Chiara Gasparetti 2 , Martina Andberg 2 , Harry Boer 2 , Anu Koivula 2 , Kristiina Kruus 2 , Juha ... observed for MaL, that may determine the properties of these asco-laccases at high protein concentrations. Database Structural data are available in the Protein Data Bank d...

Ngày tải lên: 14/02/2014, 18:20

... atoms) and r j is the standard deviation of B factors. The normalized B factors have a zero mean and unit variance. All atoms that satisfy B z ‡ 4 are treated as outliers and discarded. After ... importin -a nuclear import pathway, provided that CLIC4 can undergo a conforma- tional rearrangement that exposes the NLS in an extended conformation. Database Structural data are available in...

Ngày tải lên: 14/02/2014, 19:20

... to structure and catalysis. Biochemistry 48, 3417–3424. 31 Nakamura T, Matsumura H, Inoue T, Kai Y, Uegaki K, Hagihara Y, Ataka M & Ishikawa K (2005) Crystallization and preliminary X-ray ... Inoue T, Matsumura H, Kobayashi A, Hagihara Y, Uegaki K, Ataka M, Kai Y & Ishikawa K (2006) Crystal structure of thioredoxin peroxidase from aerobic hyperthermophilic archaeon Aeropyru...

Ngày tải lên: 14/02/2014, 22:20

... phytate with pH optima in the acidic range. They consist of two domains, a large a ⁄ b domain and a small a domain with the catalytic site at the interface of the two domains [4,5]. HAPs can initi- ate ... substrate-free AppA the C a atoms are 2.41 A ˚ apart, whereas for the substrate-free PhyK and the substrate-loaded AppA the averaged distance is only 1.87 A ˚ . Distinct c...

Ngày tải lên: 16/02/2014, 09:20

... alpha- sarcin: a study of relationships. Meth Enzymol 341, 335–351. 3 Lacadena J, A ´ lvarez-Garcı ´ a E, Carreras-Sangra N, Herrero-Gala ´ n E, Alegre-Cebollada J, Garcı ´ a- Ortega L, On ˜ aderra ... were analyzed in a semi-automated iterative manner by cyana 2.1 [38]. The NOE coordinates and intensities used as input for automated analysis were generated auto- matically by Spark...

Ngày tải lên: 18/02/2014, 08:20

... that the SEA domain functions by orienting the active site cleft of DESC1 toward plasma and ⁄ or extracellular spaces and away from the cell surface and ⁄ or the extracellular matrix. The SEA ... growth factors and proteinase inhibitors. Biol Chem 380, 473–483. 17 Kataoka H, Uchino H, Asada Y, Hatakeyama K, Nabeshima K, Sumiyoshi A & Koono M (1997) Analy- sis of tissue factor and...

Ngày tải lên: 19/02/2014, 00:20

... Ivanova N, Sorokin A, Anderson I, Galleron N, Candelon B, Kapatral V, Bhattacharyya A, Reznik G, Mikhailova N, Lapidus A et al. (2003) Genome sequence of Bacillus cereus and comparative analysis with ... 276–280. 7 Handa N, Terada T, Kamewari Y, Hamana H, Tame JRH, Park S-Y, Kinoshita K, Ota M, Nakamura H, Kuramitsu S et al. (2003) Crystal structure of the con- served protein TT1542...

Ngày tải lên: 19/02/2014, 00:20

... Stability and structural analysis of alpha-amylase from the antarctic psychrophile Alteromonas haloplanctis A2 3. Eur J Biochem 222, 441– 447. 51 Almog O, Gonzalez A, Klein D, Greenblatt HM, Braun S & ... cold adaptation at a molecular level. Structure 6, 1503–1516. 9 Leiros I, Moe E, Lanes O, Smalas AO & Willassen NP (2003) The structure of uracil–DNA glycosylase from...

Ngày tải lên: 19/02/2014, 16:20