Báo cáo khoa học: Three novel carp CXC chemokines are expressed early in ontogeny and at nonimmune sites ppt

Tài liệu Báo cáo khoa học: A novel c-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1 ppt

... A novel c-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine byArthrobacternicotinovoranspAO1Calin B. Chiribau1, Cristinel Sandu1, ... redanionic ﬂavin semiquinone was formed for more than 99%during the reaction, indicating that the redox potentials ofthe two couples (oxidized/semiquinone and semiquinone/hydroquinone) are separated ... o-dianisidine (Sigma) and 10 lgÆmL)1of MABO. Thereaction was initiated by the addition of substrate, and theincrease in absorption at 430 nm caused by the oxidation ofo-dianisidine was followed in...

Báo cáo khoa học: A novel gene, fad49, plays a crucial role in the immediate early stage of adipocyte differentiation via involvement in mitotic clonal expansion docx

... adipogenesis. Proc Natl Acad SciUSA 100, 850–855.39 Ponting CP (1996) Novel domains in NADPH oxidasesubunits, sorting nexins, and PtdIns 3-kinases: bindingpartners of SH3 domains? Protein Sci 5, 2353–2357.40 ... a solid line and the fourSH3 domains are underlined by dotted lines.Ten polyproline motifs that represent poten-tial SH3 binding sites are boxed. (B) Sche-matic structure of mouse and humanFAD49. ... studieshave demonstrated that PX domains are importantphosphoinositide-binding modules with varying lipid-binding speciﬁties, although speciﬁcity for phosphati-dylinositol 3-phosphate [PI(3)P] appears...

Tài liệu Báo cáo khoa học: Degradation of tropoelastin by matrix metalloproteinases – cleavage site speciﬁcities and release of matrikines pptx

... 150 mm; Dionex) and elution using a linear binarygradient: 5–65% ACN in 0.1% formic acid in 35 min,maintenance at 65% ACN for 10 min, and 65–5% ACN in 10 min.The typical operating conditions ... in tropoela-stin participating in covalent linkages that are crucialfor the formation of mature elastin [78]. As elastincross-links are not enzymatically degraded and thestructure of Lys is ... referred to asmatrilysin 1, is mainly expressed by epithelial cells and processes various ECM constituents, such as collagens,gelatin, and laminin, and also non-ECM proteins,including pro-tumor...

Tài liệu Báo cáo khoa học: Disulﬁde bridge regulates ligand-binding site selectivity in liver bile acid-binding proteins ppt

... corresponding to the unbound ligand and to binding sites 1 and 2 are indicated as U, 1, and 2, respectively. The satellite peaks of site 1 and site 2 are also marked. Asterisks indicate exchange ... apo and holoforms of T91C L-BABP indicated that the regionsmostly affected by binding are mainly located at theC-terminal part of the protein, at the level of Lys76,Thr81, and Val82 (strand ... Comparison of CSPinduced by complex formation in wild-type and T91CL-BABP (Fig. 6A) indicated that the same proteinregions are affected by ligand binding, conﬁrming aconserved binding mode. A few...

Tài liệu Báo cáo khoa học: The cellulosomes from Clostridium cellulolyticum Identiﬁcation of new components and synergies between complexes ppt

... Major components CipC, Cel9E and Cel48F are indicated; bands analysed using MS methods are numbered; theasterisks indicate a band containing a nonsecreted protein identiﬁedby ion-trap MS ⁄ ... [17]. In C. cellulolyticum, the scaffolding protein containseight cohesin modules that potentially bind to 62 dock-erin-bearing proteins. Previous studies have suggestedthat any CipC cohesin ... analysed using Native PAGE. In all fractions, asingle major diffuse band was observed, which showedthat the proteins present in all the fractions were in a‘cellulosome state’ [20] and that anion-exchange...

Tài liệu Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide pptx

... proteins, and to theexpression of pro- and in ammatory cytokines. Thereis evidence that the heteromeric guanine nucleotide-binding regulatory protein (G protein) is also involved in TLR4 activation. ... After ligand binding, the receptor undergoes aconformational change. Ligands include hormones,biogenic amines, histamine, serotonin and lipid deri-vates, but also immunological and in ammatory ... no regulation of RGS1 and RGS2 mRNA, indicating that poly(I:C) can only acti-vate genes via a TRIF-dependent pathway. Looking at downstream signalling events after stimulation, theinvolvement...

Tài liệu Báo cáo khoa học: Various secretory phospholipase A2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells docx

... cases, intense staining of the granulation tissue in the sublining interstitium was evident. Staining of the granulation tissue and lymphaggregates (red arrow) is magniﬁed (Eb). Synovial lining ... inactive RA sections (Fig. 4A), it was intense in VSMC in three distinct active RA sections (Fig. 4Ba–c). In contrast, staining of synovial lining cells and sublining interstitum (Fig. 4Ba–c), as well ... Intense sPLA2-V immuno-reactivity was observed in wide areas of three activeRA sections. In all cases, sPLA2-V staining was evi-dent in synovial lining cells and especiallly in sublininggranulation...

Tài liệu Báo cáo khoa học: Transactivation properties of c-Myb are critically dependent on two SUMO-1 acceptor sites that are conjugated in a PIASy enhanced manner pptx

... seems to increase the fraction of Myb species in the insoluble part after subnuclear fractionation, whichindicates that sumoylation might be involved in modulatingthe protein–protein interactions ... sumoy-lation in an in vitro conjugation system shows that c-Myb isa good substrate for SUMO-1 conjugation and thatmodiﬁcation of the two sites are interdependent, and (c) atwo-hybrid screening ... c-mybnullmutationdisplay severe hematopoietic defects leading to in uterodeath at E15 [2,3]. The c-Myb protein consists of anN-terminal DNA-binding domain (DBD), a central trans-activation domain...

Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx

... nucleotides mediate a reduction in the elongation rate and in uence the accuracy oftranslation. Early results indicated that (p)ppGpp affecttranslation by inhibition of elongation factors Tu, Ts and G[17–19]. ... reveals that EF-Ts is anelongated molecule containing four domains: the N-ter-minal domain; the core domain; the dimerization domain; and the C-terminal module [9]. The dimerization domain(residues ... been shown to stimulate the nucleotideexchange in EF-Tu by increasing the dissociation of GDP and binding of GTP [26]. In addition, the binding ofkirromycin and EF-Ts to EF-Tu are mutually exclusive...

Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx

... September2009)doi:10.1111/j.1742-4658.2009.07380.xPolypyrimidine tract-binding protein (PTB) is a widely expressed RNA-binding protein with multiple roles in RNA processing, including the splic-ing of alternative exons, mRNA stability, mRNA localization, ... Poly-pyrimidine tract-binding protein promotes insulinsecretory granule biogenesis. Nat Cell Biol 6, 207–214.10 Kosinski PA, Laughlin J, Singh K & Covey LR (2003)A complex containing polypyrimidine ... We are currently investigating whether PTB is one of theimportant regulators for the G2⁄ M phase in ES cells.Cell proliferation and differentiation are highly coor-dinated processes during...

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