... investigated the metal-binding reactivity of the isolated a domain of human metallothionein isoform 1a, with specificemphasis on resolving the debate concerning the cooperative nature of the metal-binding ... binding for M2+(M ¼ Zn, Cd) initially to the a domain, thereby lead-ing to formation of the M4a cluster prior to formation of the M3b cluster, demonstrating that the individualbinding ... the meta-llation mechanisms of the individual domains, in the hope, initially, of simplifying the interpretation of the metallation details of the two-domain protein. The results presented here...