Báo cáo khoa học: Evidence for the presence of ferritin in plant mitochondria pdf

... which were then subjected to trypsin d igestion. The intensity of the immuno- labeled band was lower in the presence of the detergent, demonstrating that ferritin is localized inside the mito- chondrial ... against the a/b-subunit and PAAF was comparable (Fig. 2B). This indicates that both the a/b-subunit and the ferritin are still retained in Mpl, thus confirming...

Ngày tải lên: 16/03/2014, 18:20

... [27]. Substrate binding adjacent to the Fe(II) is proposed to weaken binding of the remaining coordinated water, thus enabling the binding of oxygen [28,29]. Oxidative decarboxylation of 2OG then produces ... rapid in the presence of the longer protein substrate than with CODD peptide, indicating that inefficient substrate binding is probably not the cause of the sl...

Ngày tải lên: 23/03/2014, 03:20

... repre- sent the last subunits incorporated into the bc 1 com- plex. On the other hand, the absence of Qcr9p in the DQCR9 ⁄ DQCR10 strain prevented the binding of ISP (Fig. 4B). Interestingly, in the ... comparable amounts in both yeast strains. Therefore, the reason for the disap- pearance of the bc 1 dimer in the yeast strain in which Qcr10p is missing...

Ngày tải lên: 18/02/2014, 08:20

... domain, including domain B and the C-terminal domain C for rBAT proteins (669 residues on average); (b) the cata- lytic TIM barrel domain, including domain B and the C-terminal domain C for GH13 enzymes ... is the lack of domain B protruding out of the TIM barrel in the place of loop 3 connecting the b3-strand to the a3-helix [9,21]. Sharing domain B by rBATs and G...

Ngày tải lên: 18/02/2014, 13:20

... varying amounts of BTH and for different times as indicated: (B) 0.05 lg of BTH, 5 min incuba- tion; (C) 0.5 lg of BTH, 5 min incubation; (D) 5 lg of BTH, 5 min incubation; (E) 5 lg of BTH, 10 min ... mm NaCl by incubating at 37 °C for 3 h. Again, there was no evidence for the production of a specific product; instead, the reaction mixture consisted of oligosaccharide...

Ngày tải lên: 29/03/2014, 08:20

... by modulating the degradation rate of mRNAs. In the present study we investigated the effect of UV light on the stability of mRNAs expressed using the tet-off system which allows the termination of only ... expected, the expression of each of the mutants strongly interfered with the stabilization induced by MKK6 2E , a selective activator of p38 MAP kinase. I...

Ngày tải lên: 31/03/2014, 08:20

... investigated the metal-binding reactivity of the isolated a domain of human metallothionein isoform 1a, with specific emphasis on resolving the debate concerning the cooperative nature of the metal-binding ... binding for M 2+ (M ¼ Zn, Cd) initially to the a domain, thereby lead- ing to formation of the M 4 a cluster prior to formation of the M 3 b cluster, demonstr...

Ngày tải lên: 19/02/2014, 00:20

... of E103, in the binding of the twin-arginine motif in the signal peptide. The absence of any translocation defects leads us to conclude that these residues are not in fact essential for translocation ... synthesis with cleavable, N-ter- minal signal peptides that almost invariably contain an essential twin-arginine motif in the N-terminal domain [9,10]. They then int...

Ngày tải lên: 19/02/2014, 17:20

... the kinetic study of reduction of the cytochrome according to the choice of menadiol and duroquinol for spectral and kinetic studies. In uence of HOQNO on spectra The presence of the menaquinone ... fumarate reduction; the use of the quinol-binding site inhibitor HOQNO shows that the inhibitor blocks binding of MQH 2 at the Q P site [27]. Study of the...

Ngày tải lên: 07/03/2014, 15:20

... of three models for the catalytic action of plas- min in the presence of stearate. The p-nitroaniline (P) released in the course of hydrolysis of 40 l M Spectrozyme-PL by 20 nM plas- min in the ... justify the application of Model III as being the most adequate for the final evaluation of the kinetic parameters in the presence of stearate. Ev...

Ngày tải lên: 18/02/2014, 17:20