Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot

... al. (Eur. J. Biochem. 271) Ó FEBS 2004 Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines Lodovica Vergani 1 , Maura Floreani 2 , ... Determination of intracellular reactive oxygen species as function of cell density. Methods Enzymol. 352, 91–100. 44. Zuo, L. & Clanton, T.L. (20...

Ngày tải lên: 16/03/2014, 18:20

... reported in other genes, such as human and Drosophila G s a (the a-subunit of the guanine nucleotide-binding protein) isoforms [41,42]. ProSpa ¨ tzle-1 mRNA was detected in hemocytes and fat bodies of ... residue cystine-knot domain. Injection of Spa ¨ tzle-C108, but not proSpa ¨ tzle-1A, into larvae stimulated expression of several antimicrobial peptides and proteins, inc...

Ngày tải lên: 06/03/2014, 09:22

... of enzymes in the latex of Vas- concellea fruits, but in addition also results from the presence of other cysteine proteinases or isoforms. In contrast to the cysteine proteinases pre- sent in ... cysteine proteinases of Vasconcellea spp. In this investiga- tion, several cDNA sequences coding for cysteine proteinases in Vasconcel- lea · heilbornii and Vasconcellea stipul...

Ngày tải lên: 07/03/2014, 11:20

... kDa band in T cells. A Cb-specific antibody anti-Cb(SNO157) (vi) recognized the 47 kDa band in T-cells, and the 40- and 47 kDa protein bands in Jurkat cells. (C) Lin- ear depiction of Ca1, Cb1 and ... BS (2004) Induction of Cbeta splice variants and formation of novel forms of protein kinase A type II holoenzymes during retinoic acid- induced differentiation of human...

Ngày tải lên: 16/03/2014, 18:20

... UTILIZATION OF THE INFORMATION ACQUIRED DURING PREPROCESSING As shown in Figure i, the English-language processor of LDC achieves domain independence by restricting itself to (a) a domain-independent. ... negation Interestingly, most meaningful interpretations of phrases containing "non" or "not" can be obtained by inserting the retrieval r2.odule's Not...

Ngày tải lên: 17/03/2014, 19:21

... modifica- tions of the side chains of amino acids within a protein. In particular, the side chains of cysteine, histidine, methionine, tryptophan and tyrosine are susceptible to ROS-induced modifications ... 4A,B). Matching the blot with the protein map shows that PDT caused labeling of a-tubulin and b-tubulin (spots 1 and 2). The minor spot 3 probably reflects labeling of a sm...

Ngày tải lên: 23/03/2014, 15:21

... necrosis or in so-called postmitochondrial phase of apoptosis [26]; or (b) they could be involved in the initiation phase of apoptosis contributing to cell death signaling [27]. Programmed cell death ... dur- ing embryogenesis, to remove damaged cells, protect against pathogen infection, and regulate cell numbers and tissue homeostasis. Program cell death is character- is...

Ngày tải lên: 30/03/2014, 10:20

... ND6). Antioxidant defences in cybrids incubated in glucose medium The pattern of antioxidant defences was evaluated in cybrids maintained in basal culture conditions, i.e. in the presence of high ... obtained in single controls and in single cell lines affected by the same mtDNA mutation, we decided to carry out the following experiments in a single clone rep...

Ngày tải lên: 19/02/2014, 16:20

... (a) maintenance of colloid osmotic pressure; (b) binding and transport of a wide variety of metabolites including steroids, fatty acids, bilirubin, tryptophan and hemin; (c) sup- plying an amino ... the binding properties of reduced and oxidized HSA may also be different. Therefore, we investigated the relative binding proper- ties of these two types of HSA. We investigate...

Ngày tải lên: 16/03/2014, 13:20

... carried out in single- point binding assays at high initial cell density. Binding-inhibition experiments Cells grown in 12-well plates were washed once with 1 mL of DMEM and incubated in the same ... affinity and kinetics of binding of PCSK9 to LDLR on HepG2 cells at 4 °C. The extent of [ 125 I]TC-PCSK9 binding increased as cell surface LDLR density increased. Unlabeled w...

Ngày tải lên: 14/02/2014, 14:20