Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
... al. (Eur. J. Biochem. 271) Ó FEBS 2004 Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines Lodovica Vergani1, Maura Floreani2, ... Determination of intracellular reactive oxygen species as function of cell density. Methods Enzymol. 352,91–100.44. Zuo, L. & Clanton, T.L. (2002) Detection of reactive oxygen and nitrogen species ... 3–5). Antioxidant defences of q0 cell lines GSH-GSSG and GR. We measured GSH and GSSG in exponentially growing cells, as GSH content changes in thegrowth and lag phases [51]. In all q0cells studied,...
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Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt
... reported in other genes, such as human and Drosophila Gsa (thea-subunit of the guanine nucleotide-binding protein)isoforms [41,42].ProSpa¨tzle-1 mRNA was detected in hemocytes and fat bodies of ... residuecystine-knot domain. Injection of Spa¨tzle-C108, but not proSpa¨tzle-1A, intolarvae stimulated expression of several antimicrobial peptides and proteins,including attacin-1, cecropin-6, moricin, lysozyme, ... contained a 30 bp insertion in theORF, beginning at nucleotide 516. This resulted in insertion of a 10 amino acid segment (TREIDYPETI) and one substitution (Ser fi Gly) at the C-terminalend of...
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Báo cáo khoa học: Purification and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt
... of enzymes in the latex of Vas-concellea fruits, but in addition also results from the presence of othercysteine proteinases or isoforms. In contrast to the cysteine proteinases pre-sent in ... cysteine proteinases of Vasconcellea spp. In this investiga-tion, several cDNA sequences coding for cysteine proteinases in Vasconcel-lea · heilbornii and Vasconcellea stipulata were determined ... Vasconcellea spp.: (1) ahigher protein content in their latex; and (2) the pres-ence of other cysteine proteinases or isoforms in thelatex. To investigate these two hypotheses, the proteinconcentration...
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Báo cáo khoa học: Identification and characterization of novel PKA holoenzymes in human T lymphocytes pdf
... kDa band in T cells. ACb-specific antibody anti-Cb(SNO157) (vi) recognized the 47 kDa band in T-cells, and the 40- and 47 kDa protein bands in Jurkat cells. (C) Lin-ear depiction of Ca1, Cb1 and ... BS (2004) Induction of Cbeta splicevariants and formation of novel forms of proteinkinase A type II holoenzymes during retinoic acid-induced differentiation of human NT2 cells. Cell Sig-nal ... 1C and Experimental procedures). Anti-Cb2(SNO103) recognized the 47 kDa protein band in T cells and Jurkat cells, while anti-Cb(SNO157) recog-nized a 47 kDa band in T cells and Jurkat cells and...
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Báo cáo khoa học: "THE SYNTAX AND SEMANTICS OF USER-DEFINED MODIFIERS IN A TRANSPORTABLE NATURAL LANGUAGE PROCESSOR" pot
... UTILIZATION OF THE INFORMATION ACQUIRED DURING PREPROCESSING As shown in Figure i, the English-language processor of LDC achieves domain independence by restricting itself to (a) a domain-independent. ... negation Interestingly, most meaningful interpretations of phrases containing "non" or "not" can be obtained by inserting the retrieval r2.odule's Not command at an ... "layered" domains of interest [1]. Finally, the macro file contains the meanings of modifiers, roughly in the form in which they were acquired using the specification language discussed in the...
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Báo cáo khoa học: Photodynamic treatment and H2O2-induced oxidative stress result in different patterns of cellular protein oxidation ppt
... modifica-tions of the side chains of amino acids within a protein. In particular, the side chains of cysteine, histidine, methionine,tryptophan and tyrosine are susceptible to ROS-inducedmodifications ... 4A,B).Matching the blot with the protein map shows that PDTcaused labeling of a-tubulin and b-tubulin (spots 1 and 2).The minor spot 3 probably reflects labeling of a smallfraction of actin. The ... the cell. Irradiation of rat fibroblasts, loaded with both Hypo-crellin A and acetylTyr-Fluo, resulted in the labeling of cellular proteins, as shown in Fig. 3. PDT-induced proteinlabeling was...
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Báo cáo khoa học: Oxidative stress and apoptotic events during thermal stress in the symbiotic sea anemone, Anemonia viridis potx
... necrosis or in so-called postmitochondrialphase of apoptosis [26]; or (b) they could be involved in the initiation phase of apoptosis contributing to cell death signaling [27].Programmed cell death ... dur-ing embryogenesis, to remove damaged cells, protectagainst pathogen infection, and regulate cell numbers and tissue homeostasis. Program cell death is character-istic of all multicellular ... similar in ectodermal and gastrodermal cells, inhibition of DEVD-dependent protease activity by the same inhibitor waslower in ectodermal cells than in gastrodermal cells.Identification of a caspase-like...
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Tài liệu Báo cáo khoa học: Antioxidant defences in cybrids harboring mtDNA mutations associated with Leber’s hereditary optic neuropathy docx
... ND6). Antioxidant defences in cybrids incubated in glucose mediumThe pattern of antioxidant defences was evaluated in cybrids maintained in basal culture conditions, i.e. in the presence of high ... obtained in single controls and in single cell lines affected by the same mtDNAmutation, we decided to carry out the followingexperiments in a single clone representative of eachindependent line (HPC7, ... cybrid cells carrying the 11778 ⁄ ND4 and 3460 ⁄ ND1 LHON mutations, after retinoic acid-induced differentiation [13] and, more recently, in osteosarcoma-derived cybrid cell lines carrying thethree...
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Báo cáo khoa học: Identification and characterization of oxidized human serum albumin A slight structural change impairs its ligand-binding and antioxidant functions pptx
... (a) maintenance of colloid osmotic pressure; (b) binding and transport of a wide variety of metabolites including steroids,fatty acids, bilirubin, tryptophan and hemin; (c) sup-plying an amino ... the binding properties of reduced and oxidized HSA may also be different. Therefore, we investigated the relative binding proper-ties of these two types of HSA. We investigated thebinding of ... endogenous ligandwhich binds to site II, and of cefazolin (site I-ligand) and verapamil (site I and II-ligand) as exogenous lig-ands. All these ligands are known for their high bind-ing efficiencies...
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Tài liệu Báo cáo khoa học: Affinity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx
... carried out in single-point binding assays at high initial cell density.Binding-inhibition experimentsCells grown in 12-well plates were washed once with 1 mL of DMEM and incubated in the same ... affinity and kinetics of binding of PCSK9 to LDLR on HepG2 cells at 4 °C. The extent of [125I]TC-PCSK9 binding increased as cell surface LDLR densityincreased. Unlabeled wild-type and two gain -of- function ... of PCSK9 reduced binding of [125I]TC-PCSK9. The Scatchard plot of thebinding-inhibition curve was curvilinear, indicative of high-affinity and low-affinity sites for PCSK9 binding on HepG2 cells....
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