Báo cáo khoa học: Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding pot

... among the catalytic domains of Family GH 72 members. Ó FEBS 2004 Production and characterization of Gas1p (Eur. J. Biochem. 271) 3645 Characterization of recombinant forms of the yeast Gas1 protein and ... role of these residues. Here we have shown that C74 of the catalytic domain is crucial for the proper folding of Gas1p. C74 is the most N-term...

Ngày tải lên: 16/03/2014, 18:20

... the Hep3-glycoforms (see above). From the combined data, the structure in Scheme 4 is proposed for the Hex5 glycoform in the Hep4-glycoform population of NTHi isolate 1209. Characterization of ... large number of structural types were observed for the Hep3-glycoforms (15 found) and Hep4-glycoforms (13 found), all of which can be represented by structures I and II...

Ngày tải lên: 08/03/2014, 08:20

... 1988) for the use of laboratory animals, and were approved by the Scientific Committee of the Univer- sity of Leo ´ n. All efforts were made to minimize animal suffering and to reduce the number of ... translated, and to study the involvement of dmdR1 and the antisense gene (adm) in the regulation of S. coelicolor A(3)2 sporulation and antibiotic and sid...

Ngày tải lên: 07/03/2014, 02:20

... to all of the tRNA binding sites on the ribosome (i.e. A, P and E sites) [13,14]. As expected, the addi- tion of SF to the system resulted in pppGpp synthesis (Fig. 2). The speed of synthesis, ... 6E). From these results it can be concluded that tRNAs were bound in classical states with the tRNA Met f bound in the P-sites of the 30S and 50S subunits and the tRN...

Ngày tải lên: 07/03/2014, 16:20

... ± 10 l M for the recombinant form and 45 ± 2 l M for the native form). The Hill coefficients show that cooperativity for BPGA was positive (value near 1.5 for both enzymes), with both cofactors ... the curves for recom- binant and native GAPDH with a multifit using a common value of K m and different values of k cat . The estimated parameters had a value of 25...

Ngày tải lên: 23/03/2014, 20:22

... a further w ater molecule to give the carboxylic acid (Fig. 5). Therefore, the formation of the acid amide from A logically arises from the elimination of cysteine. It has been shown for the chemical ... bold) and AtNIT1-rev (5¢-ACAATTGATGATTCAACGCCCAAC 3¢). Using the BglII sites in the 5¢ overhang of AtNIT1 -for and the 3¢-noncoding region of the cDNA, the...

Ngày tải lên: 23/03/2014, 21:21

... The crystal structure of P. furiosus suggests that this loop forms part of the S 1 site. The loop is longer in Lc. lactis by four residues, and the middle of the loop is composed of charged residues (Asp36, ... prolidases in the active site area is the length of the loop structure that is contributed by the other subunit and covers the S 1 site (yellow r...

Ngày tải lên: 30/03/2014, 04:20

... shape. The second hypothesis necessary to perform Student’s t-test requires that the variance of the two samples is equal. By estimating the variance from the standard devia- tions and by using the ... lymphocytes and developing cells. On the other hand, a correlation of the intensity of these signals with metabolic parame- ters is not straightforward. Some studies n...

Ngày tải lên: 18/02/2014, 13:20

... origin and a high con- servation of this class of proteins at both the structural and functional levels. Given the multiplicity of CLPs in humans and insects [14], we hypothesized that homologues of ... (i.e. the development of the gills and the production of an adult shell), which is accom- panied by significant growth of the soft body parts [21]. The mantl...

Ngày tải lên: 19/02/2014, 00:20

... and quantitative real-time RT-PCR, the locations of the primers DN)105 and DN)9 used in ChIP assays, the locations of the primers DN+232 and DN+254 utilized in 5¢-RACE, and the locations of the ... of pGL3–basic vector and was half that of pGL3–promoter vector. These findings demonstrate the promoter activity of the 5¢-flanking region of exon 1 in DNASE...

Ngày tải lên: 19/02/2014, 06:20