Báo cáo khoa học: Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding pot
Báo cáo khoa học: Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding pot
... among the catalyticdomains of Family GH 72 members.Ó FEBS 2004 Production and characterization of Gas1p (Eur. J. Biochem. 271) 3645 Characterization of recombinant forms of the yeast Gas1 protein and ... role of these residues. Here we have shown that C74 of the catalyticdomain is crucial for the proper folding of Gas1p. C74 is the most N-terminal cysteine in Gas1p and could be required for non-native ... order to test the involvement of E161 and E262 in the active site of G as1p, we constructed twomutant His-tagged forms of Gas1 – s Gas1E161Q-H and sGas1E262Q-H (Fig. 1B). The mutant proteins were...
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Báo cáo khoa học: Structural diversity in lipopolysaccharide expression in nontypeable Haemophilus influenzae Identification of L-glycero -D-manno-heptose in the outer-core region in three clinical isolates potx
... the Hep3-glycoforms (see above). From the combined data, the structure in Scheme 4 is proposed for the Hex5 glycoform in the Hep4-glycoform population of NTHi isolate 1209. Characterization of ... large number of structural types were observed for the Hep3-glycoforms (15 found) and Hep4-glycoforms (13found), all of which can be represented by structures I and II (Hep3-glycoforms) and structure ... ESI-MSdata indicated the presence of two subpopulations of glycoforms; a major subpopulation in which the glycoformcompositions comprised three heptoses and PCho (Hep3-glycoforms), and a minor subpopulation...
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Báo cáo khoa học: Two overlapping antiparallel genes encoding the iron regulator DmdR1 and the Adm proteins control sidephore and antibiotic biosynthesis in Streptomyces coelicolor A3(2) pdf
... 1988) for the use of laboratory animals, and were approved by the Scientific Committee of the Univer-sity of Leo´n. All efforts were made to minimize animalsuffering and to reduce the number of ... translated, and to study the involvement of dmdR1 and the antisense gene (adm) in the regulation of S. coelicolor A(3)2 sporulation and antibiotic and siderophore biosynthesis.Results The Adm protein ... between the actions of the Adm and DmdR1 proteins (seebelow).Lack of both DmdR1 and Adm proteins preventsdesferrioxamine productionProduction of the siderophore desferrioxamine in the mutants and...
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Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot
... to all of the tRNA binding sites on the ribosome(i.e. A, P and E sites) [13,14]. As expected, the addi-tion of SF to the system resulted in pppGpp synthesis(Fig. 2). The speed of synthesis, ... 6E).From these results it can be concluded that tRNAswere bound in classical states with the tRNAMetfboundin the P-sites of the 30S and 50S subunits and the tRNAPhebound in the A-site of the ... trans-ferase that synthesizes (p)ppGpp. Production of thisalarmone results in a down-regulation of stable RNAsynthesis (rRNA and tRNA) and up-regulation of the synthesis of mRNAs encoding enzymes...
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Báo cáo khoa học: Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Kinetic evidence for conformation changes upon association with the small protein CP12 pptx
... ± 10 lM for the recombinant form and 45 ± 2 lM for the native form). The Hill coefficients show that cooperativity for BPGA waspositive (value near 1.5 for both enzymes), with bothcofactors ... the curves for recom-binant and native GAPDH with a multifit using acommon value of Km and different values of kcat. The estimated parameters had a value of 25 ± 2 lM for the Km, and the ... also performed. The common value of Kmwas 143 ± 15 lM and the kcat for recombinant and native GAPDH were equal to 114 ± 6 s)1 and 42 ± 3 s)1, respectively. The distribution of the residu-als...
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Báo cáo khoa học: Characterization and synthetic applications of recombinant AtNIT1 from Arabidopsis thaliana doc
... a further w ater molecule to give the carboxylic acid (Fig. 5). Therefore, the formation of the acidamide from A logically arises from the elimination of cysteine.It has been shown for the chemical ... bold) and AtNIT1-rev (5¢-ACAATTGATGATTCAACGCCCAAC3¢). Using the BglII sites in the 5¢ overhang of AtNIT1 -for and the 3¢-noncoding region of the cDNA, the AtNIT1cDNA was inserted in-frame in the ... ¯uorideb176523EDTAb18829610 94100 97a Activity of the reference, 37%;b activity of the reference, 82%.Fig. 2. Experimentally determined rates for the hydrolysis of 3-ind-olylacetonitrile (100 mg protein L)1) (A)...
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Báo cáo khoa học: Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate specificity by metal cations, and allosteric behavior of the peptidase pdf
... The crystal structure of P. furiosussuggests that this loop forms part of the S1site. The loop is longer in Lc. lactis by four residues, and the middle of the loop is composed of charged residues (Asp36, ... prolidases in the active site area is the length of the loop structure thatis contributed by the other subunit and covers the S1site (yellow ribbon for P. furiosus and cyan ribbon for Lc. lactis ... kinetic characterization of LAB physiological activity. As a consequence, thereis little information on the expression of recombinant prolidases, the functionality of each residue or protein engineering...
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Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc
... shape. The second hypothesis necessary to performStudent’s t-test requires that the variance of the two samplesis equal. By estimating the variance from the standard devia-tions and by using the ... lymphocytes and developing cells. On the other hand, a correlation of the intensity of these signals with metabolic parame-ters is not straightforward. Some studies noted that the signal intensity of ... Fig. 3A) to the spectralfitting of samples without ML signals. The presence of these peaks was paralleled by the existence of lipidcross peaks in the 2D spectra (Table 1 and Fig. 3B). The cross...
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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx
... origin and a high con-servation of this class of proteins at both the structural and functional levels. Given the multiplicity of CLPsin humans and insects [14], we hypothesized thathomologues of ... (i.e. the development of the gills and the production of an adult shell), which is accom-panied by significant growth of the soft body parts[21]. The mantle edge governs shell formation and body ... Metamorphosis represents the ulti-mate stage of oyster development, and is characterizedby the degeneration of larval tissues, such as the velum and the foot, and the remodelling of larval tissues...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
... and quantitativereal-time RT-PCR, the locations of the primers DN)105 and DN)9used in ChIP assays, the locations of the primers DN+232 and DN+254 utilized in 5¢-RACE, and the locations of the ... of pGL3–basic vector and was half that of pGL3–promoter vector. These findingsdemonstrate the promoter activity of the 5¢-flankingregion of exon 1 in DNASE1. Deletion of the upstream end of the ... numbers over the diagrams indicate the position of the corresponding nucleotides relative to the translation start site, and the numbersin parentheses below the diagram show the position of the corresponding...
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