Báo cáo khoa học: An a-proteobacterial type malate dehydrogenase may complement LDH function in Plasmodium falciparum Cloning and biochemical characterization of the enzyme potx

... malate dehydrogenase may complement LDH function in Plasmodium falciparum Cloning and biochemical characterization of the enzyme Abhai K. Tripathi 1 , Prashant V. Desai 2 , Anupam Pradhan 1 , Shabana ... by semiquantitative analysis of transcripts by Northern blotting and also by quantification of the enzyme protein by Western blotting. Expression of...

Ngày tải lên: 16/03/2014, 18:20

... chaperones and play a role in the unfolding of substrates and their translocation into the cavity of the cylinder of the proteins themselves [14]. In the past decade, although ATP-dependent proteases of ... by an N-terminal domain, a central ATPase domain which includes an SSD (sensor- and sub- strate-discrimination) domain, and a C-terminal protease domain....

Ngày tải lên: 16/03/2014, 16:20

... using a box plot. The line inside each box is the median; the upper and lower limits of the box are the 75th and 25th percentiles, respectively, and the vertical bars above and below the box indicate ... and preva- lent cancers in women and a leading cause of cancer- related death [11]. As in other common cancers, the formation and progression of breast...

Ngày tải lên: 22/03/2014, 21:20

... nondenaturing PAGE. Posi- tions of the top and middle bands coincided well with the two bands observed with the purified enzyme. Thus, it was suggested that the middle and top minor bands of the Ôcomponent ... showed that the upper band consisted of the a, b and c subunits in a molar ratio of 1.0 : 1.0 : 1.2. The lower band was composed of the a and c sub...

Ngày tải lên: 21/02/2014, 03:20

... regulated independently by the main fuel sources in muscle and by insulin through directly altering the expression of the human PDK2 and PDK4 isoforms. In addition to our findings, insulin has been ... returning transcript abundance of PDK2 and PDK4 to (minus fatty acids, minus insulin) values (Table 2). The ability of insulin alone to regulate PDK2 and PDK4 transcrip...

Ngày tải lên: 07/03/2014, 17:20

... minireview, the expression of relaxin-3 in the brain, and particularly its functions, including the stress response and food intake, are described. Expression of relaxin-3 in the brain Relaxin-3 ... expressed in the brain and testis. In rodent brain, ana- tomical studies have revealed its predominant expression in neurons of the nucleus incertus of the do...

Ngày tải lên: 29/03/2014, 21:20

... types is, the headword of the current node (type1 ), the headword of the parent node (type2 ), the headword of the grandpa node (type3 ), the first word in the objective word sequence (type4 ), the first ... first left brother of the parent 0.2505 1.5603 0.6145 5.2 The analysis to the influence of the structural relation and the structural distance...

Ngày tải lên: 20/02/2014, 18:20

... plant. Further structural analyses of the enzyme promise to provide novel insights that will aid in understanding and engineering the substrate and product specificity of the CHS superfamily type III ... containing 10% glycerol and 0.2 m KCl at a flow rate of 1.0 mLÆmin )1 . Enzyme reaction The standard reaction mixture contained 54 lmol of star- ter-CoA, 108 lmol...

Ngày tải lên: 07/03/2014, 10:20

... motor. This is the starting point for a detailed analysis of the stucture and function of the rotor of the Na + F 1 F 0 -ATP synthase from A. woo- dii, the first containing V 0 - and F 0 -like ... An intermediate step in the evolution of ATPases ) the F 1 F 0 -ATPase from Acetobacterium woodii contains F -type and V -type rotor subunits and is capable of...

Ngày tải lên: 23/03/2014, 09:20

... significant role in the product determination in these mutants, probably by blocking the elongation of the prenyl chain of the products, but in an incomplete manner. We hypothesize that type III ... the enzyme activity of the S140H and S140Y mutants was decreased to 54 and 37% of that of the wild -type HPS, respectively, while the other mutants showed...

Ngày tải lên: 23/03/2014, 17:21