Báo cáo khoa học: Analysis of the transcarbamoylation-dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE pot

... transcarbamoylation reaction catalyzed by HypF The formation of the HypE- thiocyanate involves ﬁrst the carbamoylation of the C -terminal c ysteine of HypE b yinteraction with HypF, then the release of HypF and ... future whether they can transfer the carboxamido moiety to the HypC · HypD complex. Analysis of the transcarbamoylation reaction catalyzed by the HypF proteinWhen the activity of the HypF protein ... transferred to the thiol of the C-terminal cysteine of HypE followed by itsATP-dependent dehyd ration to the thiocyanate [12]. In the course of the reaction HypF has to dock to the HypE protein and this...

Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by ﬂuorescence correlation spectroscopy and ﬂuorescence recovery after photobleaching doc

... mea-sure the mobility of the components in the PGCs. The medaka embryo was peeled off the chorion, and the segment containing the part of PGCs was excised forobservation by microscopy and FCS ... constants of 11.7, 12.9 and 5.4 lm2Æs)1, respectively, in the part of the cytoplasmoutside of the granules. FRAP analysis in the gran-ules provided typical recovery curves of these fusion proteins: ... on the same setup of the laser scanning microscope as used for FCS analysis. The detection gain was adjusted to the ﬂuorescence of the GFP fusion proteins almost at the saturation level of the detector,...

Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt

... [32]. The Lsm1 and Lsm8 polypeptides are most similar in the regions of the Sm1 and Sm2 motifs. These motifsform the Sm-fold, the hallmark of the Sm-like pro-teins, consisting of a ﬁve-stranded ... investigate the requirement for different domains of the Lsm1 and Lsm8 proteins in their function and localization, we created a series of mutant and hybrid proteins. We deleted or exchanged their N- and ... sequences from other Lsm subunits, most likely the neighbouring Lsm2p and ⁄ or Lsm4p. Alternatively,Lsm1p and Lsm8p may affect the conformation of other subunits and ⁄ or of the entire complex,...

Báo cáo khoa học: Analysis of the NADH-dependent retinaldehyde reductase activity of amphioxus retinol dehydrogenase enzymes enhances our understanding of the evolution of the retinol dehydrogenase family pot

... enzymes. The most signiﬁcant difference between the amphi-oxus and the other retinal reductases was, nevertheless,their preference for the NADH cofactor. To ourknowledge, these are the ﬁrst ... out for 15 min at 37 °Cin0.5 mL. The amount of protein used in the reaction mixturewas 15 lg. The reaction was terminated by the addition of an equal volume of cold methanol supplemented with20 ... chromosome 3 and mouse chromosome 2,which would be paralogous to chromosomes 12, 7 and 10, respectively [29]. The overall analysis, examining the topology of the phylogenetic tree and the positionof...

Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot

... 2A). The binding of raptor to these two variants was similar (Fig. 1D),implying that the loss of raptor binding on removal of the ﬁrst 17 residues may be accounted for simply by the loss of the ... deﬁne the RAIP motif [25]. The use of the rat protein also has the advantage that there is no cross-reactivity of the (P)Ser64 antibody with other sites, which is a compli-cating feature of the ... (HIF1a [21]) and the proline-richAkt-substrate 40 kDa (PRAS40 [22–24]).Although the TOS motifs in these proteins resembleone another, there are a number of differences betweenthem, and it is...

Báo cáo khoa học: Analysis of the in planta antiviral activity of elderberry ribosome-inactivating proteins potx

... structurefound in the large rRNA of prokaryotic and eukaryoticribosomes [1,2]. At present, they are subdivided on the basis of the structure of the genes and the corresponding proteins into holo-RIPs and ... K. (1987) The mechanism of action of ricin and related toxic lectins on eu-karyotic ribosomes. The site and the characteristics of the mod-iﬁcation in 28 S ribosomal RNA caused by the toxins. ... in the SNA-V and SNLRP transformants (lanes 6 and 8), but not in the SNA-I and SNA-IV transformants(lanes 2 and 4). The lack of enzymatic activity in the SNA-ITable 1. RIP-catalysed release of...

Báo cáo khoa học: Analysis of the stability of the spermadhesin PSP-I ⁄ PSP-II heterodimer Effects of Zn 2+ and acidic pH pdf

... [24], and the C1sprotease of the C1 complex of complement [25]. Thus, the Trp band can be regarded as a characteristicﬁngerprint of the native fold of PSP-I and PSP-II. The near-UV CD spectra of ... induced by Zn2+was reversed by the addi-tion of EDTA to the sample (Fig. 2A). On the otherhand, no signiﬁcant decrease in the heterodimer stabil-ity was observed in the presence of 4 mm ... asrevealed by radioactivity monitoring and SDS ⁄ PAGEfollowed by autoradiography of the material elutedFig. 2. DSC proﬁles of the thermal denaturation of PSP-I ⁄ PSP-II.Effect of Zn2+(A) and pH...

Báo cáo khoa học: Analysis of the effect of potato carboxypeptidase inhibitor pro-sequence on the folding of the mature protein pot

... min; then 10 lLofincreasingconcentrations of PCI or ProNtPCI were added and the measures were continued for 2 min. The slope of the ﬁrst part of the assay corresponded to mo and the slope of the ... in vitro and in vivo [7]. There are notmany examples of the role of the pro-regions in smalldisulﬁde-rich proteins. In these proteins, the folding processdiffers from that of larger proteins ... pro-region. Under the conditions of the experiment, the values obtained were similar for mature PCI and the ProNtPCI mutant proteins, indicating that the pro-region of PCI affects neither the expression...

Báo cáo khoa học: Analysis of the RNA degradosome complex in Vibrio angustum S14 potx

... Band 4 showed more RNase E and PNPase than RhlB and enolase. By contrast,Bands 1, 2, 3 and 5 showed roughly equivalentamounts of each of these proteins in the four samples.Interestingly, Band ... ineach band, indicating that the bands excised from the BN-PAGE gel are composed of different proteins. (C) Western blot of the seconddimension SDS ⁄ PAGE separation of the excised bands. The blot ... Furthermore, as they often require the overexpression and ⁄ or tagging of the RNAdegradosome components, the complexes formed maynot truly represent what is occurring in the cell. Inview of these...

Báo cáo khoa học: Analysis of the region for receptor binding and triggering of oligomerization on Bacillus thuringiensis Cry1Aa toxin potx

... mutant toxins and the analysis of their binding activity to the receptor toinvestigate the role of mutated amino acid residues; (b) the analysis of the inﬂuence of the modiﬁcation of the cysteine ... osmotic swelling and bursting of the cell, leading to disruption of the midgut tissue and death of the insect [16]. The three-dimensional structure of Cry1A toxins hasbeen revealed by X-ray crystallography ... sequence of the oligomeric band usingS373C, and obtained the two sequences GIFGP and GPSQW. These are sequences from residues 66 and 69, respectively, both of which are located in the loopAB200116976612...

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