Báo cáo khoa học: NMR structure of the thromboxane A2 receptor ligand recognition pocket pot

... further definition of the ligand recognition pocket on the extracellular side of the receptors. This has become a major obstacle to the further understanding of the molecular mechanism of the ... conformation change of the receptor and triggering the coupling of the receptor with the G protein in the intracellular domain s. The first step of bindi...

Ngày tải lên: 16/03/2014, 18:20

... (B) structure alignment of the fragment 4–7 of the 13 ensemble structures of AII to the fragment 3–6 of the X-ray structure of AII. Fig. 6. Structure of a representative folded conformer of AII ... Lennard- Jones potentials are used. From the family of the 48 structures, 13 structures were selected having the best allowed regions in the Ramachandran plot...

Ngày tải lên: 20/02/2014, 23:20

... [26]. These findings lead to the hypothesis that Fe-bound ApeSOD mimics the product-inhibited form and the shift of Tyr39 sup- presses the release of the peroxide product. This may be one of the ... the ter- tiary structure of ApeSOD has not been elucidated. In the present study, for the first time, we describe the crystal structure of ApeSOD. In particular, we...

Ngày tải lên: 14/02/2014, 22:20

... Lys224– Tyr228 (the back of the pocket) and the disulfide bridge Cys191–Cys220 (the front of the pocket) (Fig. 4A). The backbones of these segments form a deep hydrophobic pocket with the negatively ... according to the residue in the mid- point of the respective loop, as shown in Fig. 2. To the east of the active site the 37- and 60-loops border the S2¢...

Ngày tải lên: 19/02/2014, 00:20

... corresponding to the N-terminus of the a2- helix and to the preceding loop results in a rmsd of 1.0 A ˚ for the Ca atoms. Thus, the movement of the a2 helix accounts for 23% of the rmsd value (i.e. for ... determines the shape of the active site entry. (e) The structure of the active site is essentially identical with the active sites of the MshB a...

Ngày tải lên: 19/02/2014, 00:20

... one-turn helix on the other face. It is thought that the two inner strands of the b-sheet lie within the major groove of the DNA and that a hydrophobic pocket formed by the side chains of Y123 and ... [18]. The structure of the helical coil a 2 /a 3 allows us to interpret the consequences of the six mutations. As P153(152) and G162(161) are buried in the pr...

Ngày tải lên: 21/02/2014, 00:20

... regrowth upon the optic nerve crush, and also expressed in the germinal neuroepithelium of retina, which generates new neurons throughout the lifespan of the fish [11]. The cloning of the RICH proteins ... structures. The lowest-energy structure from the RICH NMR ensemble is used for the overlay. (D) The surface of the RICH catalytic domain shows several negat...

Ngày tải lên: 07/03/2014, 10:20

... towards the interior of the protein. The space that in the wild-type molecule is occupied by the large hydrophobic side chain of Fig. 3. Structure ensemble of HPr(I14A). The average structure of the 10 ... was computed as the ratio of the s tandard deviations of the chemical shifts of the amide nitrogen and p roton nuclei. Results Determination of th...

Ngày tải lên: 07/03/2014, 16:20

... part, and (b) the variability of the angle of approach of the inhibitor relative to the catalytic cleft of the enzyme. The latter factor may reflect not so much the geometry of the catalytic site ... PW), despite the lack of overall sequence similarity. The role of the proline residue appears to be to maintain the specific shape of the loop. The aroma...

Ngày tải lên: 16/03/2014, 04:20

... relative orientations of the a and b domains. In one struc- ture, the catalytic cysteines face each other; in the other, the catalytic residues of the a domain face away from the a¢ domain. The crystal structures ... mm of monomers at 30 °C. Most of the 1 H- 15 N HSQC signals of the dimeric form of PDI-bb¢ coincide with the signals of the monomeric form or...

Ngày tải lên: 16/03/2014, 04:20