Báo cáo khoa học: Structural characterization of photosystem II complex from red alga Porphyridium cruentum retaining extrinsic subunits of the oxygen-evolving complex docx

... Structural characterization of photosystem II complex from red alga Porphyridium cruentum retaining extrinsic subunits of the oxygen-evolving complex Ladislav Bumba1,2, ... on the presence of other extrinsic proteins [44], whereas effective binding of red algal cyt c550to the red algal PSII requires the presence of all of the other extrinsic proteins [18]. The ... overlap of the extrinsic subunits in the d ifferent binding of thesideviewstothecarbonsupportﬁlm.DiscussionHere we report the isolation o f the dimeric PSII core complex f rom t he red alga P. cruentum...

Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

... even in the absence of Ca2+. Studies on the disruption of the holoenzyme produced two functionalcomplexes, the acd complex and the cd complex dem-onstrating that CaM interacts with the kinase ... regulated by the binding of extrinsic calmodulin (CaM). The c subunit is the catalytic subunit and the d subunit is an intrinsic mole-cule of CaM that binds to the enzyme even in the absence of Ca2+[2]. ... T2). The R1 and R2 values were plotted againstFig. 4. (Top) Overlay of two spectra from the titration of Ca2+⁄ CaMwith PhK5 peptide. The red spectrum shows the position of peaksprior to the...

Báo cáo khoa học: Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus inﬂuenzae pot

... HepHepb|Hex2-Hex-Hep-AnKdo-ol|Hep|HepaMajor isomer of the Hex3 glycoform (estimated from the intensity of the fragments in MS2experiments).bMinor isomer of the Hex3 glycoform (estimated from the intensity of the fragments inMS2experiments).Fig. ... PEtnresiduewasdemonstratedtobelinked to O-3 of HepIII as the 31P resonance at d )0.50correlated to the signals from H-3 of HepIII (d 4.33) and the methylene proton pair of PEtnII (d 4.17). Correlationsbetween the signal at ... responsible for most of the steps in the biosynthesis of the OS portion of their LPS molecules.Molecular structural studies of LPS from H. inﬂuenzaestrains [9–23] have resulted in a structural model...

Báo cáo khóa học: Structural characterization of the human Nogo-A functional domains Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal cord regeneration ppt

... National University of Singapore, Singapore The recent discovery of the Nogo family of myelin inhibitorsand the Nogo-66 receptor opens up a very promising avenuefor the development of therapeutic agents ... observation,together with results from secondary structure predictions,offered the rationale to study the structure of Nogo-40 afterits intrinsic helix-forming propensity is stabilized by the introduction ... for the p eptide.NMR s pectroscopy was further utilized to explore the structural properties of Nogo-40. The very narrow reson-ance dispersion of amide protons ( 0.7 p.p.m) and the lackof...

Báo cáo khoa học: Structural characterization of the lipopolysaccharide O-polysaccharide antigen produced by Flavobacterium columnare ATCC 43622 potx

... beingcomposed of aL-FucN component originating from the O-PS residue A and the D-FucN from the reduced residue B. The isolation of optically pure 2-amino-2,6-dideoxy-D-glucose (D-QuiN), the major reduction ... of note that the structure of the LPS O-antigen of F. columnare differs structurally from the LPS O-antigen of the ﬁsh pathogen Flavobacterium psychrophilium [21],which is a linear polymer of ... andJ1,28.8 Hz. Thecorrelation of H2C tothe C2C at55.4 p.p.m.is consistent with the presence of a C2 acetamido substituent,and the lack of a proton at C6, considered in conjunctionwith the long-range...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: photosynthetic electron transfer from photosystem I to NADP+ doc

... Alanine-scanning of the 50’s loop in the Clostridium beijerinckii ﬂavodoxin:evaluation of additivity and the importance of interac-tions provided by the main chain in the modulation of the oxidation-reduction ... highlydependent on the approach and colinear orientation of the N5 of the ﬂavin, the hydride to be transferred,and C4 of the nicotinamide. In FNR, displacement of the C-terminal Tyr appears to be required ... play inthese events. The primary function of photosystem I(PSI) is to reduce NADP+to NADPH, which is thenused in the assimilation of CO2[2,3]. In plants, thisoccurs via reduction of the soluble...

Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx

... independent of the binding of the miniﬁber to the penton base, because it is attached at the C-terminal extremity of the foldon domain and isremote from the penton base-binding region. The system ... ﬁberloss, the low micromolar afﬁnity of the penton basefor ﬁber protein minimizes reattachment of the ﬁberto the viral particle. The altered conformation of the ﬁber-binding site in the hAd2 ... is the measured anisotropy value, AFis the aniso-tropy of the free miniﬁber, ABis the anisotropy of the mini-ﬁber bound to the penton base, [LigB] is the concentration of the bound ligand...

Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modiﬁcation sites and structural requirements for substrate processing and subcellular localization pptx

... products, the cleavage site protected by the presence of ubiquitin was Ser341, located in the ‘ﬁngers’ region of the catalytic core domain involvedin the recognition of the ubiquitin core. These ... Based on these predictions, we stud-ied the localization of deletion mutants of the ﬁrst 20,50 and 70 amino acids of USP7. Surprisingly, thesevariants were found preferentially in the cell ... recently the possible origin of the substrate afﬁnity divergences compared to USP7 in adetailed analysis of the interaction of USP2 catalyticcore with ubiquitin. Despite the higher KM, the cata-lytic...

Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt

... In these equations, q is the solventdensity,t is the partial speciﬁc volume of the Iba protein,x is the angular velocity, R is the gas constant and T the absolute temperature. Aris the ... with the consensussequence, the crucial glutamate in the last position of the motif (the ‘)Z’ position) [28] is substituted mostlyby serines or glycines. The coordination of Ca2+by the )Z ... Residues 12–16 of the N-termi-nus and 20–23 of the C-terminus are missing in thesestructures. For the Iba1 crystal structures, this obser-vation was attributed to a partial truncation of the proteins....

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... respectively, of that of HPRT. This low activitycould be explained by the fact that PRTFDC1 has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of ... strength [20].In the present study, we report the structural andfunctional characterization of the human HPRThomolog PRTFDC1. To shed light on the potentialfunction of PRTFDC1, the protein was ... composed of b2 andb9 and an a-helix from the C-terminus (Fig. 1A). The two subunits in the asymmetric unit, together with twosymmetry-related subunits, form a plausible tetramersimilar to the tetramer...

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