Báo cáo khoa học: Malonyl-CoA decarboxylase (MCD) is differentially regulated in subcellular compartments by 5¢AMP-activated protein kinase (AMPK) Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique potx

... regulated in subcellular compartments by 5¢AMP-activated protein kinase (AMPK) Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique Nandakumar Sambandam, Michael ... enzyme activity. In vitro phosphorylation of MCD by AMPK using lysates of cells overexpressing either MCD or CA-AMPKa 1(312) H9c2...

Ngày tải lên: 16/03/2014, 16:20

... meta-iodobenzylguanidine; PD128763, 3,4-dihydro-5-methylisoquinoline; PGE 2 , prostaglandin E 2 ; PI3 -kinase, phosphatidylinositol 3 -kinase; PtdInsP 3 , phos- phatidylinositol-3,4,5-trisphosphate; PRK, protein kinase ... labeled proteins were detected in quiescent SMC (Fig. 4), however, both the number of bands and the labeling intensity were increased in growing cells. The most...

Ngày tải lên: 17/03/2014, 09:20

... domain binding motif in their C-termini. PDZ domains have been established as sites for protein protein inter- action and play a central role in organizing diverse cell signaling assem- blies. In ... Dynabeads (Invi- trogen) and DynaMag (Invitrogen). Any interaction was detected using MUPP1 antibodies. Immunohistochemistry Mice were raised and maintained according to governmen...

Ngày tải lên: 18/02/2014, 13:20

... (M) is the histidine-tagged mCyp4x1 protein, and its mobility is indica- ted by a line; the mobility of the Cyp4x1 specific band is indicated by an arrow. (C) Quantification of Cyp4x1 in brain microsomes. Thirty ... specific binding, the antisera were preincubated with purified recombinant Cyp4x1 protein, resulting in the ablation of detection of the 55-kDa protein in brain...

Ngày tải lên: 19/02/2014, 07:20

... was generated by standard PCR with pKH7 as template DNA and using KpnIandSacI for transferring the PCR product into pKH7 (resulting in plasmid pMM9). The mutation aP280C was generated using a ... y alanines, one novel cysteine in c (K108C) was introduced and a His 6 -tag at the N-terminus of subunit b was added [13]. MM10 contained t wo additional cysteines in positions aP280C...

Ngày tải lên: 19/02/2014, 16:20

... Molecular Genetics, Weizmann Institute of Science, Israel Antizyme is a polyamine-induced cellular protein that binds to ornithine decarboxylase (ODC), and targets it to rapid ubiquitin-independent ... degradation. DISCUSSION Antizyme is a unique cellular regulatory protein that is both regulated by polyamines and regulates polyamine metabo- lism in a feedback loop. Anti...

Ngày tải lên: 22/02/2014, 04:20

... non-shivering thermogenesis in brown adipose tissue by catalysing proton leak acti- vated by long-chain fatty acids and inhibited by purine nucleotides [4]. UCP2 is widely expressed in many tis- sues ... proposed to involve higher production of intracellular ROS [14]. On the other hand, studies in cells overexpressing UCP2 have rein- forced the belief that UCP2 plays a r...

Ngày tải lên: 05/03/2014, 23:20

... unsaturated C 18 fatty acids. This hypothesis is supported by in silico co-expression analysis, showing that CYP77A4 is co -regulated with a stearoyl acyl carrier protein desat- urase and a putative epoxide ... into the cutin. In this context, it is interesting to note that inhibition studies allowed LeQueu et al. [49] to demon- strate the involvement of a peroxygenase in...

Ngày tải lên: 07/03/2014, 03:20

... which is in the direct vicinity of the N(5) atom of the isoalloxazine ring system and a likely candidate in a position for the abstraction of hydrogen from the substrate. Single-mutant proteins in ... two invariant serine residues at positions 16 and 127 of the Neurospora crassa chorismate synthase with alanine, producing two single-mutant proteins (Ser16Ala and Ser127Ala) and...

Ngày tải lên: 07/03/2014, 05:20

... multimerization is located on 26 amino acids of the N-terminus [9,10]. In this work, we investigated this region in detail, introducing dele- tions and point mutations. All mutations incorporated in the ... as for mapping domains of CPs involved in this process. The recombinant NLPs, with an average length of 50 nm, represent 20–30% of the total purified proteins. The remaining pr...

Ngày tải lên: 07/03/2014, 05:20