Báo cáo khoa học: Malonyl-CoA decarboxylase (MCD) is differentially regulated in subcellular compartments by 5¢AMP-activated protein kinase (AMPK) Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique potx
Báo cáo khoa học: Malonyl-CoA decarboxylase (MCD) is differentially regulated in subcellular compartments by 5¢AMP-activated protein kinase (AMPK) Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique potx
... regulated in subcellular compartments by 5¢AMP-activated protein kinase (AMPK) Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique Nandakumar Sambandam, Michael ... enzyme activity. In vitrophosphorylation of MCD by AMPK using lysatesof cells overexpressing either MCD or CA-AMPKa1(312) H9c2 cells overexpressing MCD were lysed with buffercontaining 75 mMKCl, ... 14.2 nmolÆmin)1Æmg)1 in Ad .MCD + Ad.CA- AMPK cells, P ¼ 0.058; Fig. 2Biv). MCD expression and activity in subcellular fractionsof H9c2 cells overexpressing both MCD and AMPK Previous studies...
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Báo cáo khoa học: Endogenous mono-ADP-ribosylation mediates smooth muscle cell proliferation and migration via protein kinase N-dependent induction of c-fos expression potx
... meta-iodobenzylguanidine;PD128763, 3,4-dihydro-5-methylisoquinoline; PGE2, prostaglandinE2; PI3 -kinase, phosphatidylinositol 3 -kinase; PtdInsP3, phos-phatidylinositol-3,4,5-trisphosphate; PRK, protein kinase ... labeled proteins were detected in quiescent SMC (Fig. 4), however, both the number ofbands and the labeling intensity were increased in growing cells. The most prominent increases were in bands ... response factor (SRF) [30]. Protein kinase C-related kinase 1 (PRK1), also termed protein kinase N (PKN), and PRK2 have been identified as criticalintermediates linking Rho to SRF [31]. Mitogen...
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Tài liệu Báo cáo khoa học: Olfactory receptor signaling is regulated by the post-synaptic density 95, Drosophila discs large, zona-occludens 1 (PDZ) scaffold multi-PDZ domain protein 1 pptx
... domain binding motif in their C-termini.PDZ domains have been established as sites for protein protein inter-action and play a central role in organizing diverse cell signaling assem-blies. In ... Dynabeads (Invi-trogen) and DynaMag (Invitrogen). Any interaction wasdetected using MUPP1 antibodies.ImmunohistochemistryMice were raised and maintained according to governmen-tal and institutional ... a rapid and specific signal transduction[9,12].PDZ domains are modular protein protein interac-tion domains, which are amongst the most abundant protein interaction domains in organisms from...
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Tài liệu Báo cáo khoa học: Cytochrome P450 Cyp4x1 is a major P450 protein in mouse brain doc
... (M) is the histidine-tagged mCyp4x1 protein, and its mobility is indica-ted by a line; the mobility of the Cyp4x1 specific band is indicated by an arrow. (C) Quantification of Cyp4x1 in brain microsomes.Thirty ... specific binding, the antisera were preincubatedwith purified recombinant Cyp4x1 protein, resulting in the ablation of detection of the 55-kDa protein in brain(Fig. 5C). This shows that the antisera ... wereassigned using the rules of Summers and Karplus [39].Main chain coordinates for residues in loops were obtained using the search method implemented in sybyl (TriposInc., St Louis, MO, USA)....
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Tài liệu Báo cáo khoa học: Rotary F1-ATPase Is the C-terminus of subunit c fixed or mobile? docx
... wasgenerated by standard PCR with pKH7 as template DNA and using KpnIandSacI for transferring the PCR productinto pKH7 (resulting in plasmid pMM9). The mutationaP280C was generated using a ... yalanines, one novel cysteine in c (K108C) was introduced and a His6-tag at the N-terminus of subunit b was added[13]. MM10 contained t wo additional cysteines in positionsaP280C and cA285C, and ... al.[9])andinthe‘hydrophobic bearing’ formed by subunits a and b aroundthe C-terminal portion of c. Molecular dynamics calcula-tions ([20] and this work) suggested the unwinding of thesingle...
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Tài liệu Báo cáo Y học: Ornithine decarboxylase-antizyme is rapidly degraded through a mechanism that requires functional ubiquitin-dependent proteolytic activity pot
... Molecular Genetics, Weizmann Institute of Science, IsraelAntizyme is a polyamine-induced cellular protein that bindsto ornithine decarboxylase (ODC), and targets it to rapidubiquitin-independent ... degradation.DISCUSSIONAntizyme is a unique cellular regulatory protein that is both regulated by polyamines and regulates polyamine metabo-lism in a feedback loop. Antizyme expression is regulated translationally ... process is unclear. The prominentnotion which is based predominantly on studies performed in vitro in degradation extracts assumes that a ntizyme is astable protein that is recycled during the...
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Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx
... non-shivering thermogenesis in brown adipose tissue by catalysing proton leak acti-vated by long-chain fatty acids and inhibited by purinenucleotides [4]. UCP2 is widely expressed in many tis-sues ... proposed to involve higherproduction of intracellular ROS [14]. On the otherhand, studies in cells overexpressing UCP2 have rein-forced the belief that UCP2 plays a role in limitingintracellular ... (1987) Some characteristicsof in ammation induced by muramyl dipeptide, endo-toxin and concanavalin A. In ammation 11, 1–11.25 Kozlov AV, Staniek K, Haindl S, Piskernik C, O¨hlingerW, Gille L,...
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Báo cáo khoa học: Arabidopsis thaliana CYP77A4 is the first cytochrome P450 able to catalyze the epoxidation of free fatty acids in plants potx
... unsaturatedC18fatty acids. This hypothesis is supported by in silicoco-expression analysis, showing that CYP77A4 is co -regulated with a stearoyl acyl carrier protein desat-urase and a putative epoxide ... intothe cutin. In this context, it is interesting to note thatinhibition studies allowed LeQueu et al. [49] to demon-strate the involvement of a peroxygenase in the forma-tion of cutin of corn.The ... [18]. In plants, fatty acids are also metabolized by cyto-chrome P450-dependent oxygenases [19], and it is possible to distinguish x-hydroxylases and in- chainhydroxylases that attack the terminal...
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Báo cáo khoa học: Replacement of two invariant serine residues in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity docx
... which is in the direct vicinity of theN(5) atom of the isoalloxazine ring system and a likelycandidate in a position for the abstraction of hydrogenfrom the substrate. Single-mutant proteins in ... twoinvariant serine residues at positions 16 and 127 of the Neurospora crassachorismate synthase with alanine, producing two single-mutant proteins(Ser16Ala and Ser127Ala) and a double-mutant protein (Ser16Ala-Ser127Ala). ... allthree mutant proteins was comparable to that of thewild-type enzyme.Binding of oxidized FMN to the mutant proteinsTo characterize the serine mutant proteins in furtherdetail, binding of the...
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Báo cáo khoa học: The F13 residue is critical for interaction among the coat protein subunits of papaya mosaic virus doc
... multimerization is located on26 amino acids of the N-terminus [9,10]. In this work,we investigated this region in detail, introducing dele-tions and point mutations. All mutations incorporated in the ... as formapping domains of CPs involved in this process. Therecombinant NLPs, with an average length of 50 nm,represent 20–30% of the total purified proteins. Theremaining protein is essentially ... Hz in the1H dimension and 1680 Hz in the15N dimension. NMR spectra were pro-cessed using NMRPipe [15]. Processing involved doublingof the15N time domain by linear prediction, zero-filling...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Báo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDETrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Kiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀM