Báo cáo khoa học: Expression of the pyrG gene determines the pool sizes of CTP and dCTP in Lactococcus lactis doc

... Expression of the pyrG gene determines the pool sizes of CTP and dCTP in Lactococcus lactis Casper M. Jørgensen, Karin Hammer, Peter R. Jensen and Jan Martinussen Bacterial Physiology and Genetics, ... through sensing of the UMP concentration in the cell [1–5]. However, PyrR is not involved in the regulation of expression of the pyrG...

Ngày tải lên: 16/03/2014, 16:20

... phosphoinositide binding [31]. The five-amino-acid insertion in the loop of Noxo1 might alter the configuration of the phospho- inositide-binding region, affecting the affinity for Expression and function ... in the testis and fetal brain. The Noxo1c protein contains an additional five amino acids in the N-terminal PX domain, a phosphoinosi- tide-binding module; t...

Ngày tải lên: 19/02/2014, 06:20

... into t he expression vector following the manufacturer’s instruction (Gateway, Invi- trogen). The correct insertion of the Rv1399c ORF was confirmed by DNA sequencing. In our hands, protein expression ... [23]. Results and Discussion Cloning and expression of Rv1399c The Rv1399c gene product encodes a 318 amino acid protein with a molecular mass of  31.7 kDa and...

Ngày tải lên: 07/03/2014, 16:20

... included the mitochondrial single- stranded binding protein (mtSSB), and the catalytic (a )and accessory (b) subunits of the DNA polymerase c (P ol c) [24,25]. Interestingly, the expression of the genes ... identification of several transcription factors and coactivators that regulate the expression of genes playing key r oles in the biogenesis of the OXPHO...

Ngày tải lên: 07/03/2014, 16:20

... calculated using the forcefield of discover3 and used to anchor the pyranose ring of the sugars into the binding sites of the lectin. The position of mannose observed in the Man–banana lectin complex ... energy over the threshold value. The docking of MeMan into the carbohydrate-binding sites of Orysata and other JRLs was performed with the program insig...

Ngày tải lên: 14/03/2014, 23:20

... unique band corresponding to the 70-kDa protein demonstrating that this protein is the recombinant laccase. Northern blot analysis was performed in order to check the laccase gene expression during ... construction. Considering these results, the expression vector pLac1-B was selected to characterize the recombinant laccase from A. niger . Immunodetection of the recombin...

Ngày tải lên: 17/03/2014, 11:20

... family and contains a di-zinc binding domain and a small domain that is inserted in the middle of the metal- binding domain and mediates catalysis. The di-zinc Fig. 6. Metal effects on the enzymatic ... His80 in the vicinity of the zinc center and is assumed to clamp the imidazolium ring of His80. Glu149 is in the immediate vicinity of the Glu150 of...

Ngày tải lên: 23/03/2014, 06:20

... steps of the sponge protein purification: during cell lysis and pro- tein binding as well as during the column washing steps. A less viscous lysate was observed in the pres- ence of the nuclease. Inspection ... could be involved in determining the unusual product profile of the pre- paration. The specific activity of the recombinant protein was rather low, being in...

Ngày tải lên: 23/03/2014, 09:20

... detected in mucosa but, in general, ADH4 was found in outer cell layers, lining the lumen, while ADH1 was detected in the inner cell layers. Of interest were the sharp discontinuities in the expression found ... throughout the epithelium in intestinal villi and crypts of Lieberku ¨ hn (Fig. 4A,B). By IHC, ADH4 was prominent in the epithelial cells lining inte...

Ngày tải lên: 23/03/2014, 17:22

... after 210 min, exhibiting the bands of the remaining oxy complex. The 340 nm-band of NADPH decreases in proportion to the decrease of Soret band at 410 nm and to the increases of broad band spreading ... distinguishable from the Soret band of free hemin. Utilizing this difference, the stoichiometry of the heme binding reaction ratio to Syn HO-1 was examined. T...

Ngày tải lên: 23/03/2014, 20:22