Báo cáo khoa học: Effect of valine 106 on structure–function relation of cytosolic human thymidine kinase Kinetic properties and oligomerization pattern of nine substitution mutants of V106 ppt

... Effect of valine 106 on structure–function relation of cytosolic human thymidine kinase Kinetic properties and oligomerization pattern of nine substitution mutants of V106 Hanne Frederiksen*†, ... nine mutations: V106A, V106G, V106H, V106I, V106K, V106L, V106M, V106Q and V106T. We then characterized the enzymatic properties of the mutant e...

Ngày tải lên: 16/03/2014, 16:20

... linezolid and glycopeptides only, and Stenotropho- monas maltophilia, sensitive to ofloxacin only. Polymicrobial wound contamination also included contamination with fungi (for instance, Candida ... Near-drowning involved the aspiration of immersion fluids, marine and soil debris into the respiratory tract and all patients displayed signs of pneumonitis and pneumonia upon arriva...

Ngày tải lên: 25/10/2012, 10:41

... attentional failures after they had been working for more than 16 hours on the traditional schedule [2]. Letter Effect of intern’s consecutive work hours on safety, medical education and professionalism Christopher ... hours or more consecutive work are unsafe. Efforts to reduce work hours should focus first and foremost not on the frequency of extended-duration work shifts, b...

Ngày tải lên: 25/10/2012, 10:45

... Characterization of siRNA–TRBP complex formation after TRBP knockdown. Fig. S4. Additional characterization of Dicer and TRBP complexes. Fig. S5. Effect of a terminal mismatch at the guide strand 5¢ end on ... impact of silencing TRBP and Dicer on the function of the fully paired and mismatched sequences, we wished to examine Fig. 1. Effect of guide strand 5¢ end mis...

Ngày tải lên: 18/02/2014, 06:20

... whether the P-loop moves upon oxidation of the catalytic cysteine is the ability of the conserved active site arginine to move Effect of ionic strength and oxidation on PhyAsr R. J. Gruninger ... absence of ligand, the WPD loop of PTP1B and RPTPa adopts an open (inactive) conformation, and upon ligand bind- ing, it adopts a closed (active) conformation (Fig. 3A). In oxidiz...

Ngày tải lên: 18/02/2014, 18:20

... role of this region in the transcription of the Ig-b gene. Deletion of region III decreased the expres- sion of Ig-b mRNA to 40% of the expression in prede- letion cells (Fig. 4A). Region III ... hybridization. Position of the probes, the size and position of restriction fragments hybridizable with the probe, and exon ⁄ introns are shown as in Fig. 2A. Positions of the DT4...

Ngày tải lên: 19/02/2014, 16:20

... several cytoplasmic ligands. It is activa- ted by millimolar concentrations of ATP and micromo- lar Ca 2+ , and inhibited by higher concentrations (> 100 lm)ofCa 2+ and by millimolar Mg 2+ . ... pore [10]. Results about the effect of the luminal (trans) calcium on the activity of the RyR1 are controversial depending on experimental conditions. Inhibition of the channel...

Ngày tải lên: 19/02/2014, 16:20

... hydrolysis of the substrates harboring these mutations was more pronounced for the wild-type enzyme and nonactive-site mutants (L90M and M46L) than for the active-site mutants. Consequently, mutation of ... constant showed a strong correlation with the volume of the P1¢ residue (correlation coefficient R ¼ 0.90) and the number of hydrophobic contacts the side chain formed wit...

Ngày tải lên: 21/02/2014, 03:20

... term correspondences are estimated, in terms of both computational complexity and the performance of precise estimation of bilingual term correspondences. 1 Introduction Translation knowledge acquisition ... situ- ation. One limitation of the corpus-based trans- lation knowledge acquisition approach is that the techniques of translation knowledge acquisition heavily rely on avai...

Ngày tải lên: 22/02/2014, 02:20

... excitation and 520 nm emission filters. The wells were loaded with solutions of a fixed concentration of preformed quadruplex (50 nm) and increasing concentra- tions of complementary strand (0–1 ... is composed of quartets and interspersed loops, in the genome it is also flanked on each side by numerous bases. The effect of loop length and composition on quadruplex confor...

Ngày tải lên: 07/03/2014, 02:20