Báo cáo khoa học: Membrane distribution of epidermal growth factor receptors in cells expressing different gangliosides doc

... microdomains (GEM) of the plasma membrane and to be involved in the regulation of epidermal growth factor receptor (EGFr or ErbB1) activity. To gain further insight into the mechanisms involved in ... masses) of recombinant proteins are indicated. (B) Protein profile of the gradient, visualized by Ponceau S staining of the nitrocellulose membrane. Ó FEBS 2004 EGF recep...

Ngày tải lên: 16/03/2014, 16:20

... Hung, L.M. & Lin, C.C. (1997) cGMP-elevating agents suppress proliferation of vascular smooth muscle cells by inhibit- ing the activation of epidermal growth factor signaling pathway. Circulation ... Spain Nitric oxide (NO • ) strongly inhibits the proliferation of human A431 tumour cells. It also inhibits tyrosine phos- phorylation of a 170-kDa band corresponding to the...

Ngày tải lên: 17/03/2014, 03:20

... diglucosamine ring vibrational bands at 1170 and 1045 cm )1 , allowing calculation of the inclination angle of the diglucosamine ring plane with respect to the membrane plane [14]. In Table 1, the ... liposomes corresponding to the com- position of the macrophage membrane, phosphatidyl- choline, phosphatidylserine, phosphatidylethanolamine and sphingomyelin in a molar ratio of...

Ngày tải lên: 23/03/2014, 13:20

... showing that P2X 2–4 receptor proteins were induced by nerve growth factor in these same cells [45,46]. In an additional cellular model system for PD, consisting of SN4741 inducible dopaminergic ... likely depending on the intensity of the microglia reaction, the kind of stimuli received and other local factors, including cross-talk with neighbouring neuronal cells, or induc...

Ngày tải lên: 30/03/2014, 02:20

... onformational changes involving protein loops or backbone. T hus, the role of OBP in signal triggering remains unresolved. 3 Keywords: crystal str ucture; domain swapping; odorant- binding protein; olfaction. Odorant-binding ... func- tional human retinol-bin ding protein i n Escherichia coli using a secretion v ector. Biochem. J. 296, 209–215. 41. Monaco, H.L. ( 2000) The transthyretin–r...

Ngày tải lên: 30/03/2014, 15:20

... local- ization of iNOS to apical domains of intestinal epithe- lial cells. Particulate fraction association of iNOS in vivo In order to determine the distribution of iNOS in intestinal epithelial cells in ... enrichment in iNOS activity within the particulate fraction of epithelial cells. Apical distribution of iNOS in intestinal epithelial cells To get mor...

Ngày tải lên: 19/02/2014, 16:20

... extracellular matrix; laminin binding protein; topological distribution; integral membrane protein. One of the primary events in the initiation of a disease is thought to be the attachment of the causative ... anti-(laminin IgG), goat anti-(rabbit IgG), BCIP and NBT. Lane 3, transferred proteins were incubated with BSA instead of laminin. Fig. 3. Radiolabeled laminin binding to (...

Ngày tải lên: 21/02/2014, 00:20

... tropomyosin and actin, the IANBD probe retained its typical responses to changes in Ca 2+ and S1 binding (Fig. 6). Fitting binding curves to obtain binding parameters is difficult in the case of Ca 2+ because ... troponin-tropomyosin: inhibition of actomyosin ATPase activity without inhibition of myosin binding to actin. J Biol Chem 256, 575–578. 36 Tobacman LS & Adelstein RS (19...

Ngày tải lên: 07/03/2014, 09:20

... FEBS 235 A B C D 0 min 2 min 10 min 30 min 0 min 2 min 10 min 30 min 0 min 2 min 10 min 30 min 0 min 2 min 5 min 30 min 0 min 10 min 20 min 30 min 0 min 2 min 10 min 30 min 0 min PKC-α PKC-δ PKC-α PKC-δ PKC-α PKC-δ PKC-α PKC-δ 10 ... pattern of substitution can contribute to the orientation, either sn-1orsn-2, of the insertion of the DAG lactone into the binding cleft of the C1 domain...

Ngày tải lên: 15/03/2014, 09:20

... the untreated pellet after correcting for adhering medium, which was determined from the amount of associated [ 14 C]inulin. Measurement of protein content This was by a bicinchoninic acid assay kit (Sigma). Statistical ... must rely on an internal, luminal pool of CoASH. Transport of L -carnitine into sealed microsomal vesicles Transport of L -[ 3 H]carnitine was studied by measure...

Ngày tải lên: 16/03/2014, 16:20