Báo cáo khoa học: A (1ﬁ3)-b-D-glucan recognition protein from the sponge Suberites domuncula Mediated activation of ﬁbrinogen-like protein and epidermal growth factor gene expression pot

... (ENSAN5_ANGA, XP_312116.1) and (BACBP_ANGA, CAA04496.1), as well as the GLUBP from the lobster Homarus gam-marus (GLUBP_HOGAM, CAE47485.1) and the crayﬁsh Pacifastacus leniusculus (GLUBP_PACLE, ... usingGENEDOC[38].Recombinant EGF precursor and production of antibodies The sponge SDEGFI-PREC sequence was isolated byPCR using the forward primer, f1 [5¢-CCATGGAGAAGATTCTAGCAACAGTCAATTCAAATGAC-3¢ (the NcoI ... W.E.G. & Gamulin, V. (2000) The mitogen-activated protein kinase p38 pathway is conserved inmetazoans: cloning and activation of p38 of the SAPK2 subfamily from the sponge Suberites domuncula. ...

Báo cáo khoa học: A new phospholipase A2 isolated from the sea anemone Urticina crassicornis – its primary structure and phylogenetic classiﬁcation pptx

... hunting and defense) of the sea anemone Adamsia carciniopados(AcPLA2) [20]. Nested RT-PCR with degenerateprimers and RACE was used to clone the PLA2 from this animal. AcPLA2contains a putative ... ampliﬁed and digitized at a sampling rate of 1 kHz,using a data acquisition system (Digidata 144 0A; MolecularDevices, Sunnyvale, CA, USA). The ﬁnal concentrations of UcPLA2 and AtxC in the bath ... 695–706.16 Grotendorst GR & Hessinger DA (1999) Puriﬁcation and partial characterization of the phospholipase A 2 and co-lytic factor from sea anemone (Aiptasia pallida)nematocyst venom. Toxicon...

Báo cáo khoa học: A novel metallocarboxypeptidase-like enzyme from the marine annelid Sabellastarte magniﬁca – a step into the invertebrate world of proteases pdf

... granulifera, Cassiopeaxamachana, Condylactys gigantea, Gorgonia ventalina,Lebrunia danae, Palythoa caribaeorum, Physalia phy-salis, Plexaura homomalla, Stichodactyla helianthus and Zoanthus pulchellus); ... radia-tus and Lima scabra); four species of Chordata (Pallu-sia nigra, Microcosmus gamus, Molgula occidentalis and Pyura vittata); 11 species of Cnidaria (Bartholo-mea annulata, Budonosoma granulifera, ... the kingdom Methazoa(Phyla: Annelida, Urochordata, Echinodermata, Cnidaria,Mollusca, Artropoda) were collected in the north coast of Havana and classiﬁed by Cuban specialists at the NationalInstitute...

Báo cáo khoa học: "A Preference-first Language Processor Integrating the Unification Grammar and Markov Language Model for Speech Recognition-ApplicationS" potx

... hypotheses and significantly reducing the search space. Therefore the global structural synthesis capabilities of the unification grammar and the local relation estimation capabilities of the ... recognition system. The language processor consists of a language model and a parser. The language model properly integrates the unification grammar and the Markov language model, while the ... correct rate of recognition can be as high as 98.3%. This indicates that the language processor based on the integration of the unification grammar and the Markov language model can in fact be...

Báo cáo khoa học: a-1 Antitrypsin binds preprohepcidin intracellularly and prohepcidin in the serum pdf

... is synthesized as an 84-amino-acid (AA)preprohormone, and is present in the plasma as a mature 25-AA peptide and as a 60-AA prohormoneform. Maturation is facilitated by the serine peptidasefurin. ... Dongiovanni P, Piperno A, Fracanzani AL,Maggioni M, Rametta R, Loria P, Casiraghi MA,Suigo E, Ceriani R et al. (2006) Alpha 1-antitrypsinmutations in NAFLD: high prevalence and associationwith altered ... First, the screening of protein interactions of preprohepcidin as bait and human liver cDNAlibrary as target was carried out on Luria–Bertani(LB) agar plates in the presence of Gal-X. In cases of protein protein...

Báo cáo khoa học: A steady-state competition model describes the modulating effects of thrombomodulin on thrombin inhibition by plasminogen activator inhibitor-1 in the absence and presence of vitronectin ppt

... orcan result in cleavage of the inhibitor and release of activeenzyme. A decreased overall inhibition rate can thus be the result of a shift of the rate constants of the branched part of mechanism, ... DE, USA). Human a- thrombin puriﬁed from plasma was a gift of G. Tans(University of Maastricht, the Netherlands). Construction, expression, and activation of recombinant prothrombinvariants were ... in a signiﬁcant increase in surface-bound mass if formation of Fig. 2. TM does not alter the distribution of the cleavage and substratepathway. Analysis by SDS/PAGE of the products of the reaction...

Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the speciﬁcity and relative binding energy of RNA-binding proteins ppt

... the performance of the potential will only increase with the size of the structural database and as terms areadded to the model to account for protein and RNAintra-molecular interactions that are ... under-standable barrier to the quantitative application of computational approaches to the problem of protein RNA recognition. However, we have previously dem-onstrated that a statistical hydrogen ... to muta-tions of individual amino acids at protein RNA interfaces. Thus, this workdemonstrates that statistical models allow the quantitative analysis of protein RNA recognition based on their...

Tài liệu Báo cáo khoa học: A genetic screen identifies mutations in the yeastWAR1 gene, linking transcription factor phosphorylation to weak-acid stress adaptation docx

... 5¢-CTTCAACAACTCTCTTTACAAAATG AGGTATGTTATTGCGTTATTTTG-3¢ This studyF763Mas 5¢-CAAAATAACGCAATAACATACCTCAT TTTGTAAAGAGAGTTGTTGAAG-3¢ This studyR764Ds5¢-CCCTTCAACAACTCTCTTTACAAATTTTATGTTATTGCGTTATTTTGTC-3¢ ... studyR764Das 5¢-GACAAAATAACGCAATAACATAAAATTTGTAAAGAGAGTTGTTGAAGGG-3¢ This studyYeast weak organic acid stress adaptation C. Gregori et al.3104 FEBS Journal 274 (2007) 3094–3107 ª 2007 The Authors ... prim-ers WAR1-GSTs (5¢-AAGAATTCTCCATGGGGGAAATGTCGCATACCATA-3¢) and WAR1-GSTas (5¢-CTGCAGTCAAATGTCGACATTCATGAAAAGGTCTGTCC-3¢),as described elsewhere [40]. The PCR product was digestedwith EcoRI and...

Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx

... l-dopa as cofactor toeliminate the characteristic lag period of tyrosinases[8,20,21]. Figure 3 shows the rate of TH activity as a function of the concentration of l-dopa cofactor addedto the assay ... The most reliable way of exploring this is comparison of crystal structure data. The only data so far available are for sweet potato(Ipomoea batata) catechol oxidase [26]. The catalyticcopper ... 3754–3760.13 Sanchez-Amat A & Solano F (1997) A pluripotent poly-phenol oxidase from the melanogenic marine Alteromo-nas sp shares catalytic capabilities of tyrosinases and laccases. Biochem...

Tài liệu Báo cáo khoa học: "A HYBRID APPROACH TO REPRESENTATION IN THE JANUS NATURAL LANGUAGE PROCESSOR" pot

... E.W., Ayuso, D.M., and Scha, R. The Syntax and Semantics of the JANUS Semantic Inter- pretation Language. In Research and Development in Natural Language Understanding as Part of the Strategic ... plurals follows Scha [25]. The operators KIND and SAMPLE follow Cad.son's analysis [10] of the semantics of bare plurals. THE, as an operator, takes three arguments: a variable, a sort ... from the class associated with A to the class associated with B. If no such role exists, the search is for a role relating the nearest ancestor of the class of A to any ancestor of the class...

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